公开(公告)号：US07332317B2

公开(公告)日：2008-02-19

申请号：US10168402

申请日：2000-12-22

申请人： Kenji Omori ,  Keizo Yuasa ,  Jun Kotera ,  Kotomi Oda ,  Hideo Michibata

发明人： Kenji Omori ,  Keizo Yuasa ,  Jun Kotera ,  Kotomi Oda ,  Hideo Michibata

IPC分类号： C12N9/16 ,  C07H21/04

CPC分类号： C12N9/16 ,  C12Q2521/119

摘要： The present invention is to provide a novel phosphodiesterase and a gene thereof, specifically, Type 11 phosphodiesterase (PDE11) and a gene thereof, more specifically, a phosphodiesterase selected from (A) a protein having an amino acid sequenced shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39, and (B) a protein having an amino acid sequence shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39 in which one or several amino acids are deleted, substituted or added, and having an activity of hydrolyzing a cyclic nucleotide, and a gene thereof, and a method of characterizing, identifying and selecting a phosphodiesterase inhibitor by using the same.

摘要翻译： 本发明提供一种新的磷酸二酯酶及其基因，特别是11型磷酸二酯酶（PDE11）及其基因，更具体地，提供选自（A）具有SEQ.ID所示的氨基酸测序的蛋白质的磷酸二酯酶。 SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6或SEQ ID NO：39，和（B）具有SEQ ID NO：2，SEQ ID NO：2所示氨基酸序列的蛋白质 其中一个或几个氨基酸被缺失，取代或加入并且具有水解环状核苷酸的活性及其基因的SEQ ID NO：4，SEQ ID NO：6或SEQ ID NO：39 ，以及通过使用它们来表征，鉴定和选择磷酸二酯酶抑制剂的方法。

公开(公告)号：US08012731B2

公开(公告)日：2011-09-06

申请号：US11960999

申请日：2007-12-20

申请人： Kenji Omori ,  Keizo Yuasa ,  Jun Kotera ,  Kotomi Oda ,  Hideo Michibata

发明人： Kenji Omori ,  Keizo Yuasa ,  Jun Kotera ,  Kotomi Oda ,  Hideo Michibata

IPC分类号： C07H21/00 ,  C12N15/00 ,  C12Q1/68 ,  C12N1/21 ,  C12N5/10 ,  C12N9/16 ,  C12P21/00

CPC分类号： C12N9/16 ,  C12Q2521/119

摘要： The present invention is to provide a novel phosphodiesterase and a gene thereof, specifically, Type 11 phosphodiesterase (PDE11) and a gene thereof, more specifically, a phosphodiesterase selected from (A) a protein having an amino acid sequenced shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39, and (B) a protein having an amino acid sequence shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39 in which one or several amino acids are deleted, substituted or added, and having an activity of hydrolyzing a cyclic nucleotide, and a gene thereof, and a method of characterizing, identifying and selecting a phosphodiesterase inhibitor by using the same.

摘要翻译： 本发明提供一种新的磷酸二酯酶及其基因，特别是11型磷酸二酯酶（PDE11）及其基因，更具体地，提供选自（A）具有SEQ.ID所示的氨基酸测序的蛋白质的磷酸二酯酶。 SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6或SEQ ID NO：39，和（B）具有SEQ ID NO：2，SEQ ID NO：2所示氨基酸序列的蛋白质 其中一个或几个氨基酸被缺失，取代或加入并且具有水解环状核苷酸的活性及其基因的SEQ ID NO：4，SEQ ID NO：6或SEQ ID NO：39 ，以及通过使用它们来表征，鉴定和选择磷酸二酯酶抑制剂的方法。

公开(公告)号：US20090325207A1

公开(公告)日：2009-12-31

申请号：US11960999

申请日：2007-12-20

申请人： Kenji OMORI ,  Keizo Yuasa ,  Jun Kotera ,  Kotomi Oda ,  Hideo Michibata

发明人： Kenji OMORI ,  Keizo Yuasa ,  Jun Kotera ,  Kotomi Oda ,  Hideo Michibata

IPC分类号： C12Q1/42 ,  C12N9/16 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N9/16 ,  C12Q2521/119

摘要： The present invention is to provide a novel phosphodiesterase and a gene thereof, specifically, Type 11 phosphodiesterase (PDE11) and a gene thereof, more specifically, a phosphodiesterase selected from (A) a protein having an amino acid sequenced shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39, and (B) a protein having an amino acid sequence shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: in which one or several amino acids are deleted, substituted or added, and having an activity of hydrolyzing a cyclic nucleotide, and a gene thereof, and a method of characterizing, identifying and selecting a phosphodiesterase inhibitor by using the same.

摘要翻译： 本发明提供一种新的磷酸二酯酶及其基因，特别是11型磷酸二酯酶（PDE11）及其基因，更具体地，提供选自（A）具有SEQ.ID所示的氨基酸测序的蛋白质的磷酸二酯酶。 SEQ ID NO：2，SEQ ID NO：4，SEQ ID NO：6或SEQ ID NO：39，和（B）具有SEQ ID NO：2，SEQ ID NO：2所示氨基酸序列的蛋白质 其中一个或几个氨基酸被缺失，取代或加入并具有水解环状核苷酸的活性及其基因.ID.NO:4，SEQ.ID.NO:6或SEQ.ID.NO： 以及通过使用它们来表征，鉴定和选择磷酸二酯酶抑制剂的方法。

公开(公告)号：US20130288908A1

公开(公告)日：2013-10-31

申请号：US13990654

申请日：2011-11-30

申请人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

发明人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

IPC分类号： G01N33/68

CPC分类号： G01N33/6854 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/55 ,  C12N15/1041 ,  C12N15/1062 ,  C12N15/1075 ,  G01N33/6857

摘要： The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself, and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.

摘要翻译： 以下的（1）或（2）的多核苷酸构建体用于进行核糖体展示，CIS显示和/或mRNA显示，以便针对感兴趣的抗原筛选Fab：（1）单核细胞系包含核糖体 - 结合序列，Fab第一链编码序列，接头肽编码序列，Fab第二链编码序列和支架编码序列，并且在其3'末端还包含与Fab保持复合物所必需的结构 其自身编码，和（2）包含Fab第一链表达顺反子和Fab第二链表达顺反子的多核苷酸构建体，其各自含有核糖体结合序列，Fab第一链编码序列或Fab第二链编码序列 和支架编码序列，所述第一Fab表达顺反子在其3'末端进一步包含核糖体失速序列，所述Fab第二链表达顺反子在其3'末端还包含结构ne 维持与Fab自身编码的复合物的塞车。

公开(公告)号：US09753040B2

公开(公告)日：2017-09-05

申请号：US13990654

申请日：2011-11-30

申请人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

发明人： Yasuhiro Fujino ,  Risako Fujita ,  Kouichi Wada ,  Kotomi Oda ,  Takuya Ueda ,  Yoshihiro Shimizu ,  Takashi Kanamori

IPC分类号： G01N33/68 ,  C12N15/10 ,  C07K16/46

CPC分类号： G01N33/6854 ,  C07K16/468 ,  C07K2317/31 ,  C07K2317/55 ,  C12N15/1041 ,  C12N15/1062 ,  C12N15/1075 ,  G01N33/6857

摘要： The polynucleotide construct of (1) or (2) below is used to perform ribosome display, CIS display and/or mRNA display in order to screen a Fab against an antigen of interest: (1) a polynucleotide construct which monocistronically comprises a ribosome-binding sequence, Fab first chain-coding sequence, linker peptide-coding sequence, Fab second chain-coding sequence and scaffold-coding sequence in this order, and further comprises at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself; and (2) a polynucleotide construct which comprises a Fab first chain-expressing cistron and a Fab second chain-expressing cistron each containing a ribosome-binding sequence, a Fab first chain-coding sequence or Fab second chain-coding sequence, and a scaffold-coding sequence in this order, the first Fab-expressing cistron further comprising at its 3′-end a ribosome stall sequence, said Fab second chain-expressing cistron further comprising at its 3′-end a structure necessary for maintaining a complex with the Fab encoded by itself.