公开(公告)号：US5075236A

公开(公告)日：1991-12-24

申请号：US186078

申请日：1988-04-25

申请人： Kenji Yone ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Arata Kato ,  Satoshi Nakamura ,  Tsukio Masegi ,  Kazuo Kitai ,  Yataro Ichikawa

发明人： Kenji Yone ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Arata Kato ,  Satoshi Nakamura ,  Tsukio Masegi ,  Kazuo Kitai ,  Yataro Ichikawa

IPC分类号： G01N33/53 ,  C07K14/005 ,  C07K14/195 ,  C07K16/24 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC分类号： C07K16/241 ,  C07K2317/34 ,  Y10S436/811 ,  Y10S436/815

摘要： A method of confirming the diagnosis of Kawasaki disease in a patient which comprises assaying the patient's body fluid for the presence of elevated levels of a substance specifically bound by an anti-tumor necrosis factor monoclonal antibody.

公开(公告)号：US5028420A

公开(公告)日：1991-07-02

申请号：US235576

申请日：1988-07-26

申请人： Tsukio Masegi ,  Satoshi Nakamura ,  Kazuo Kitai ,  Masami Fukuoka ,  Kenji Yone ,  Arata Kato ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Yataro Ichikawa

发明人： Tsukio Masegi ,  Satoshi Nakamura ,  Kazuo Kitai ,  Masami Fukuoka ,  Kenji Yone ,  Arata Kato ,  Jun Suzuki ,  Noriyuki Tsunekawa ,  Yataro Ichikawa

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K1/22 ,  C07K14/00 ,  C07K14/52 ,  C07K14/525 ,  C12N1/20 ,  C12N15/28 ,  C12P21/02 ,  C12R1/19

CPC分类号： C07K14/525 ,  A61K38/00

摘要： A novel physiologically active polypeptide having antitumor activity obtained by improving human tumor necrosis factor (TNF). The amino acid sequence of the polypeptide essentially corresponds to that of human tumor necrosis factor, except that ten amino acids Nos. 1 to 10 are deleted and (Met).sub.n -Arg-Lys-Arg are added to the amino (NH.sub.2)-terminus, where n is 0 or 1. Other amino acid modifications can also be made to the polypeptide while retaining high antitumor activity. The novel polypeptide has less side effects that the natural-type TNF, particularly with respect to cytotoxicity. A a DNA fragment encoding the polypeptide, a recombinant plasmid containing the DNA fragment, a recombinant microorganism cell transformed with the plasmid, a method of producing a novel physiologically active polypeptide using the microorganism cell, and a pharmaceutical composition comprising the polypeptide as an active ingredient are also provided.