公开(公告)号：US5589335A

公开(公告)日：1996-12-31

申请号：US290001

申请日：1994-08-11

申请人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

发明人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6806 ,  C12Q1/689 ,  Y10S435/967

摘要： Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.

摘要翻译： 提供了新的试剂以及用于检测样品中靶核酸的核酸杂交测定中使用的方法。 最优选的试剂是硫氰酸胍和四甲基铵：三氟乙酸盐的混合物，并且具有许多促进靶核酸与能够结合到其上的核酸探针之间的杂交的性质，并且令人惊讶地导致超化学计量标记的现象。

公开(公告)号：US5759777A

公开(公告)日：1998-06-02

申请号：US469208

申请日：1995-06-06

申请人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

发明人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6806 ,  C12Q1/689 ,  Y10S435/967

摘要： Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.

公开(公告)号：US08618252B2

公开(公告)日：2013-12-31

申请号：US12966541

申请日：2010-12-13

申请人： Graham K. Farrington ,  Alexey Alexandrovich Lugovskoy ,  Werner Meier ,  John K. Eldredge ,  Ellen Garber

发明人： Graham K. Farrington ,  Alexey Alexandrovich Lugovskoy ,  Werner Meier ,  John K. Eldredge ,  Ellen Garber

IPC分类号： C07K1/00 ,  C07K16/00 ,  C12P21/08 ,  A61K39/395 ,  A61K39/40 ,  A61K39/00

CPC分类号： C07K16/00 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/71 ,  C07K2317/72

摘要： The compositions and methods of the present invention are based, in part, on our discovery that an effector function mediated by an Fc-containing polypeptide can be altered by modifying one or more amino acid residues within the polypeptide (by, for example, electrostatic optimization). The polypeptides that can be generated according to the methods of the invention are highly variable, and they can include antibodies and fusion proteins that contain an Fc region or a biologically active portion thereof.

摘要翻译： 本发明的组合物和方法部分地基于我们的发现，即可以通过修饰多肽内的一个或多个氨基酸残基来改变由含Fc多肽介导的效应子功能（通过例如静电优化 ）。 可以根据本发明的方法产生的多肽是高度可变的，并且它们可以包括含有Fc区或其生物活性部分的抗体和融合蛋白。