公开(公告)号：US5759777A

公开(公告)日：1998-06-02

申请号：US469208

申请日：1995-06-06

申请人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

发明人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6806 ,  C12Q1/689 ,  Y10S435/967

摘要： Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.

公开(公告)号：US5589335A

公开(公告)日：1996-12-31

申请号：US290001

申请日：1994-08-11

申请人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

发明人： Kevin R. Kearney ,  Mark L. Collins ,  John K. Eldredge ,  David V. Morrissey

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6806 ,  C12Q1/689 ,  Y10S435/967

摘要： Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.

摘要翻译： 提供了新的试剂以及用于检测样品中靶核酸的核酸杂交测定中使用的方法。 最优选的试剂是硫氰酸胍和四甲基铵：三氟乙酸盐的混合物，并且具有许多促进靶核酸与能够结合到其上的核酸探针之间的杂交的性质，并且令人惊讶地导致超化学计量标记的现象。

公开(公告)号：US06268128B1

公开(公告)日：2001-07-31

申请号：US07902517

申请日：1992-06-22

申请人： Mark L. Collins ,  David V. Morrissey

发明人： Mark L. Collins ,  David V. Morrissey

IPC分类号： C12Q168

CPC分类号： C12Q1/6813 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00497 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： The invention is a method of detecting nucleic acids in a sample using oligonucleotide probes which are noncovalently bound to solid supports for rapid, sensitive, hybridization assays. The method involves coating the support surface with a polynucleotide and then hybridizing a specific capture probe for each analyte to the polynucleotide by way of a short tail of the complementary polynucleotide. The immobilized probes are used to capture nucleic acid targets out of complex specimens for nonisotopic detection without the need for prior cell culture or purification of the target nucleic acids. A panel of tests can be run on each specimen simultaneously, a format that conserves precious samples. The assay can be readily automated, and can be conveniently run in a manual fashion on large numbers of samples in two to three hours.

摘要翻译： 本发明是使用与固体支持物非共价结合的寡核苷酸探针来检测样品中的核酸的方法，用于快速，灵敏，杂交测定。 该方法包括用多核苷酸涂覆载体表面，然后通过互补多核苷酸的短尾将每种分析物的特异性捕获探针与多核苷酸杂交。 固定化探针用于捕获复合标本中的核酸靶，用于非同位素检测，而不需要先前的细胞培养或纯化靶核酸。 可以在每个样本上同时运行一组测试，这是一种节省宝贵样品的格式。 该测定可以容易地自动化，并且可以在2-3小时内以手动方式在大量样品上方便地运行。

公开(公告)号：US5457025A

公开(公告)日：1995-10-10

申请号：US976678

申请日：1992-11-16

申请人： Mark L. Collins ,  David Gillespie ,  David V. Morrissey

发明人： Mark L. Collins ,  David Gillespie ,  David V. Morrissey

IPC分类号： C12N15/09 ,  C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/6834

摘要： Methods and kits are described that allow the efficient capture and detection of targets employing the dA-dT and related affinity pairs from samples potentially containing large amounts of poly(rA) and/or poly(dA).

摘要翻译： 描述了允许使用dA-dT的目标和潜在含有大量聚（rA）和/或聚（dA）的样品的相关亲和对的有效捕获和检测的方法和试剂盒。