利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

20 条记录 (耗时 0.024 秒)

    Immobilized oligonucleotide probes and uses thereof
    1.
    发明授权
    Immobilized oligonucleotide probes and uses thereof 失效
    固定的寡核苷酸探针及其用途

    公开(公告)号：US06268128B1

    公开(公告)日：2001-07-31

    申请号：US07902517

    申请日：1992-06-22

    申请人： Mark L. Collins ,  David V. Morrissey

    发明人： Mark L. Collins ,  David V. Morrissey

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6813 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00497 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

    摘要： The invention is a method of detecting nucleic acids in a sample using oligonucleotide probes which are noncovalently bound to solid supports for rapid, sensitive, hybridization assays. The method involves coating the support surface with a polynucleotide and then hybridizing a specific capture probe for each analyte to the polynucleotide by way of a short tail of the complementary polynucleotide. The immobilized probes are used to capture nucleic acid targets out of complex specimens for nonisotopic detection without the need for prior cell culture or purification of the target nucleic acids. A panel of tests can be run on each specimen simultaneously, a format that conserves precious samples. The assay can be readily automated, and can be conveniently run in a manual fashion on large numbers of samples in two to three hours.

    摘要翻译： 本发明是使用与固体支持物非共价结合的寡核苷酸探针来检测样品中的核酸的方法，用于快速，灵敏，杂交测定。 该方法包括用多核苷酸涂覆载体表面，然后通过互补多核苷酸的短尾将每种分析物的特异性捕获探针与多核苷酸杂交。 固定化探针用于捕获复合标本中的核酸靶，用于非同位素检测，而不需要先前的细胞培养或纯化靶核酸。 可以在每个样本上同时运行一组测试，这是一种节省宝贵样品的格式。 该测定可以容易地自动化，并且可以在2-3小时内以手动方式在大量样品上方便地运行。

    Discontinuous probe design using hybritope mapping
    2.
    发明授权
    Discontinuous probe design using hybritope mapping 失效
    不连续的探针设计使用杂交映射

    公开(公告)号：US5736327A

    公开(公告)日：1998-04-07

    申请号：US435809

    申请日：1995-05-05

    申请人： Mark L. Collins

    发明人： Mark L. Collins

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6811

    摘要： This invention provides a method of detecting or determining a binding oligonucleotide comprising a nucleotide sequence which binds within a known nucleotide sequence of a target nucleic acid using a technique called hybritope mapping. This invention also provides a method of using hybritope mapping to obtain discontinuous probes that bind to a target nucleic acid.

    摘要翻译： 本发明提供一种检测或测定包含核苷酸序列的结合寡核苷酸的方法，所述核苷酸序列使用称为杂交图谱的技术在靶核酸的已知核苷酸序列内结合。 本发明还提供了一种使用杂交子作图来获得结合靶核酸的不连续探针的方法。

    Methods for improving the sensitivity of hybridization assays
    3.
    发明授权
    Methods for improving the sensitivity of hybridization assays 失效
    提高杂交测定灵敏度的方法

    公开(公告)号：US5702896A

    公开(公告)日：1997-12-30

    申请号：US598142

    申请日：1996-02-07

    申请人： Mark L. Collins ,  Cecile Blomquist ,  Massimo Lombardo ,  John Eldredge

    发明人： Mark L. Collins ,  Cecile Blomquist ,  Massimo Lombardo ,  John Eldredge

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  C12Q1/6832

    摘要： Methods for improving the sensitivity of hybridization assays which reduce non-specific binding (NSB) and non-specific hybridization (NSH) are disclosed. The methods include a washing method utilizing tetraalkylammonium salts at high temperatures, and release methods in which a probe-target complex is released from a solid support and recaptured. Use of both the washing and release methods results in substantial reduction in NSB and NSH without performing several rounds of release and recapture of the target nucleic acids.

    摘要翻译： 公开了提高减少非特异性结合（NSB）和非特异性杂交（NSH）的杂交测定的灵敏度的方法。 这些方法包括在高温下使用四烷基铵盐的洗涤方法，以及释放方法，其中探针 - 靶复合物从固体支持物释放并重新捕获。 使用洗涤和释放方法均可大大减少NSB和NSH，而不进行几轮释放和重新捕获靶核酸。

    Closed vessel for isolating target molecules and for performing amplification
    8.
    发明授权
    Closed vessel for isolating target molecules and for performing amplification 失效
    用于分离靶分子和进行扩增的封闭容器

    公开(公告)号：US5714380A

    公开(公告)日：1998-02-03

    申请号：US622491

    申请日：1996-03-25

    申请人： Bruce P. Neri ,  John S. Curtis ,  Mark L. Collins ,  Danahey Ryan

    发明人： Bruce P. Neri ,  John S. Curtis ,  Mark L. Collins ,  Danahey Ryan

    IPC分类号： B01L3/00 ,  C12Q1/68 ,  G01N33/543 ,  G01N35/00 ,  C12M1/34 ,  C12M1/40

    CPC分类号： B01L3/502 ,  C12Q1/6846 ,  G01N33/54326 ,  G01N35/0098

    摘要： The present invention features a vessel for isolating a target in a sample. The vessel includes at least one reaction chamber, a wash system and an effluent system. The reaction chamber includes a closed cell adapted to receive a support, a sample potentially containing target and at least one first probe, and thereafter being closed. The probe is capable of associating with the support and the target to form a support-probe-target complex and sample debris upon imposition of probe binding conditions within the reaction chamber. A wash system is capable of introducing solutions into the reaction chamber for washing the support to solubilize and suspend sample debris. Upon imposition of wash conditions, solutions are allowed to enter the reaction chamber to solubilize such sample debris. An Effluent system is in communication with the reaction chamber and capable of receiving sample debris and wash solutions. The vessel receives the sample, binds the target, if present, to the support, allows wash solutions to remove sample debris, and removes wash solutions and sample debris through the effluent system, leaving target isolated on the support.

    摘要翻译： 本发明的特征在于用于分离样品中的靶的容器。 该容器包括至少一个反应室，洗涤系统和流出物系统。 反应室包括适于接收载体的密闭孔，潜在地包含靶和至少一个第一探针的样品，之后被封闭。 当在反应室内施加探针结合条件时，该探针能够与载体和靶相关联以形成载体 - 探针 - 靶复合体和样品碎片。 洗涤系统能够将溶液引入反应室中以洗涤载体以溶解和悬浮样品碎片。 当施加洗涤条件时，允许溶液进入反应室以溶解这样的样品碎片。 废水系统与反应室连通，能够接收样品碎屑和洗涤液。 容器接收样品，将目标物（如果存在）结合到载体上，允许洗涤溶液去除样品残渣，并且通过流出物系统除去洗涤溶液和样品碎片，将目标分离在载体上。