Fermentation process
    1.
    发明授权
    Fermentation process 失效
    发酵过程

    公开(公告)号:US07320884B2

    公开(公告)日:2008-01-22

    申请号:US10762895

    申请日:2004-01-22

    IPC分类号: C12P7/44 C12N1/18

    CPC分类号: C12P7/6409 C12N1/16

    摘要: A process for forming a color and odor stable polycarboxylic acid or polyhydroxy acid is provided. The process is carried out by fermenting a mixture containing a substrate which can be converted by fermentation into a polycarboxylic acid or a polyhydroxy acid, an organism capable of transforming the substrate by fermentation in a fermentation medium containing a source of carbon and energy, a source of inorganic nitrogen, a source of phosphate, a metal, a biotin which is substantially free of particulate matter and bacteria.

    摘要翻译: 提供了形成颜色和气味稳定的多元羧酸或多羟基酸的方法。 该方法通过将含有可通过发酵转化的底物的混合物发酵成多羧酸或多羟基酸,能够在含有碳源和能量源的发酵培养基中通过发酵转化底物的生物体,源 的无机氮,磷酸盐源,金属,基本上不含颗粒物质和细菌的生物素。

    Process for recovering carboxylic acids from a fermentation broth
    2.
    发明授权
    Process for recovering carboxylic acids from a fermentation broth 有权
    从发酵液中回收羧酸的方法

    公开(公告)号:US06245538B1

    公开(公告)日:2001-06-12

    申请号:US09345682

    申请日:1999-06-30

    IPC分类号: C12P740

    CPC分类号: C12P7/44

    摘要: Carboxylic acids are recovered from a fermentation broth by adjusting the pH of a fermentation broth comprised of a carboxylic acid to at least about 6.0 and then heating the pH-adjusted broth to a temperature sufficient to effect the formation of three immiscible phases, one of which is an oily phase rich in the carboxylic acid.

    摘要翻译: 通过将由羧酸组成的发酵液的pH调节至至少约6.0,然后将pH调节的肉汤加热到足以形成三个不混溶相的温度,从发酵液中回收羧酸,其中之一 是一种富含羧酸的油相。

    Fermentation process
    3.
    发明授权

    公开(公告)号:US06569670B2

    公开(公告)日:2003-05-27

    申请号:US09754158

    申请日:2001-01-04

    IPC分类号: C12N120

    摘要: A fermentation medium containing: (a) a source of metabolizable carbon and energy; (b) a source of inorganic nitrogen; (c) a source of phosphate; (d) at least one metal selected from the group consisting of an alkali metal, an alkaline earth metal, a transition metal, and mixtures thereof; and (e) biotin, substantially free of particulate matter and bacteria. A fatty material may be added to an aqueous suspension containing at least one dicarboxylic acid to modify the rheological characteristics of the suspension.

    Process for recovering carboxylic acids from a fermentation broth
    4.
    发明授权
    Process for recovering carboxylic acids from a fermentation broth 失效
    从发酵液中回收羧酸的方法

    公开(公告)号:US6143532A

    公开(公告)日:2000-11-07

    申请号:US880476

    申请日:1997-06-23

    CPC分类号: C12P7/44

    摘要: Carboxylic acids are recovered from a fermentation broth by adjusting the pH of a fermentation broth comprised of a carboxylic acid to at least about 6.0 and then heating the pH-adjusted broth to a temperature sufficient to effect the formation of three immiscible phases, one of which is an oily phase rich in the carboxylic acid.

    摘要翻译: 通过将由羧酸组成的发酵液的pH调节至至少约6.0,然后将pH调节的肉汤加热到足以形成三个不混溶相的温度,从发酵液中回收羧酸,其中之一 是一种富含羧酸的油相。

    Method for controlling biooxidation reactions
    5.
    发明授权
    Method for controlling biooxidation reactions 失效
    控制生物氧化反应的方法

    公开(公告)号:US07270947B2

    公开(公告)日:2007-09-18

    申请号:US10756240

    申请日:2004-01-13

    CPC分类号: C12P7/6409 C12P7/44 C12P7/46

    摘要: Biooxidation reactions can be controlled by a method which comprises the steps of: (1) independently adding a substrate and a co-substrate at predetermined rates to a bioxidation reaction mixture comprised of a biocatalyst; (2) measuring the oxygen consumption rate and carbon dioxide evolution rate from the reaction mixture; (3) determining the instantaneous rates of substrate and cosubstrate consumption by solving simultaneous equations relating carbon dioxide evolution rate and oxygen consumption rate to the substrate oxidation stoichiometry, the cosubstrate combustion stoichiometry, and optionally the biomass formation stoichiometry; (4) simultaneously adjusting the substrate and cosubstrate addition rates to the rates of substrate oxidation and cosubstrate consumption in order to maximize the rate of product formation while simultaneously minimizing the rate of cosubstrate usage. The method provides a rapid means of controlling fed-batch biooxidation reactions which can employ in-line techniques and is broadly applicable for diverse oxidation reactions.

    摘要翻译: 生物氧化反应可以通过一种方法来控制,该方法包括以下步骤:(1)以包含生物催化剂的生物氧化反应混合物以预定速率独立地加入底物和共底物; (2)从反应混合物中测量氧气消耗速率和二氧化碳释放速率; (3)通过求解将二氧化碳进化速率和氧耗率与二氧化碳排放速率和氧消耗速率相关的联立方程与底物氧化化学计量法,同位素燃烧化学计量法和任选的生物质形成化学计量法来确定底物和辅助消耗的瞬时速率; (4)同时调整衬底并将衬底加入速率与底物氧化和辅助消耗的速率进行调和,以最大限度地提高产物形成速率,同时最大限度地降低衬底使用率。 该方法提供了控制补料分批生物氧化反应的快速方法,其可以使用在线技术并且广泛适用于多种氧化反应。

    Process for making polycarboxylic acids
    6.
    发明授权
    Process for making polycarboxylic acids 失效
    制备多元羧酸的方法

    公开(公告)号:US5962285A

    公开(公告)日:1999-10-05

    申请号:US106611

    申请日:1998-06-23

    IPC分类号: C12P7/44 C12P7/46 C12P7/64

    摘要: Aliphatic polycarboxylic acids are made by a process comprising the steps of: (1) fermenting a beta-oxidation blocked C. tropicalis cell wherein both copies of the chromosomal POX5 gene and the chromosomal POX4A and POX4B genes are disrupted in a culture medium comprised of a nitrogen source, an organic substrate and a cosubstrate wherein the substrate is an unsaturated aliphatic compound having at least one internal carbon--carbon double bond and at least one terminal methyl group, a terminal carboxyl group and/or a terminal functional group which is oxidizable to a carboxyl group by biooxidation; (2) reacting the product of step (1) with an oxidizing agent to produce one or more polycarboxylic acids.

    摘要翻译: 脂肪族多羧酸通过包括以下步骤的方法制备:(1)发酵β-氧化阻断的热带假丝酵母细胞,其中染色体POX5基因和染色体POX4A和POX4B基因的两个拷贝在包含 氮源,有机底物和底物,其中所述底物是具有至少一个内部碳 - 碳双键和至少一个末端甲基,末端羧基和/或末端官能团的不饱和脂族化合物,其可氧化成 羧基通过生物氧化反应; (2)使步骤(1)的产物与氧化剂反应以产生一种或多种多元羧酸。

    Process for continuously splitting a glyceride into carboxylic acids and
glycerin
    7.
    发明授权
    Process for continuously splitting a glyceride into carboxylic acids and glycerin 失效
    将甘油酯连续分解成羧酸和甘油的方法

    公开(公告)号:US5830719A

    公开(公告)日:1998-11-03

    申请号:US843968

    申请日:1997-04-17

    CPC分类号: C12P7/6418

    摘要: Carboxylic acids and glycerine are made by a continuous splitting process which involves the formation of a presplitting mixture by separately adding the glyceride, an effective lipase in an amount sufficient to produce partial splitting of the glyceride, and water. The water used in the formation of the presplitting mixture is water that has been separated from the glycerin-water effluent stream from the pressure splitter and recycled. The next step involves the pressure splitting which entails mixing the partially split glyceride from the presplitter with water and heating under conditions of temperature and pressure effective to substantially complete the splitting of the glyceride into component fatty acids and a glycerin-water stream. The water is then separated from the glycerine-water stream and the water is recycled to the presplitter.

    摘要翻译: 羧酸和甘油通过连续分离方法制备,其包括通过单独加入甘油酯形成预分解混合物,该量为足以产生甘油酯部分裂解的量的有效脂肪酶和水。 用于形成预分裂混合物的水是已经从压力分配器与甘油 - 水流出物流分离并再循环的水。 下一步涉及压力分解,其需要将分解器中的部分分离的甘油酯与水混合,并在有效基本上完成将甘油酯分解成组分脂肪酸和甘油 - 水流的温度和压力的条件下加热。 然后将水从甘油 - 水流中分离出来并将水再循环到分解器中。

    Shock indicator
    10.
    发明授权
    Shock indicator 失效
    震动指标

    公开(公告)号:US07219619B2

    公开(公告)日:2007-05-22

    申请号:US10517685

    申请日:2003-06-13

    IPC分类号: G01L5/00

    CPC分类号: G01P15/036 G01P15/06 H04M1/18

    摘要: A shock indicator is described comprising (A) a base having a first side and a second side; (B) an indicator associated with the first side of the base, the indicator comprising a plurality of indicator subparts, the subparts comprising solid material arranged (i) in a first configuration when the shock indicator is in a first state prior to a shock event, and (ii) in a second configuration when the shock indicator is in a second state following a shock event; and (C) means associated with the second side of the base for attachment of the shock indicator to a surface. A method of manufacture is also provided.

    摘要翻译: 描述的冲击指示器包括:(A)具有第一侧和第二侧的底座; (B)与所述基座的第一侧相关联的指示器,所述指示器包括多个指示器子部件,所述子部件包括固体材料,所述固体材料布置成(i)当所述休克指示器在冲击事件之前处于第一状态时处于第一配置 ,以及(ii)当所述休克指示器处于休克事件之后的第二状态时处于第二配置; 和(C)与基座的第二侧相关联的装置,用于将冲击指示器附接到表面。 还提供了一种制造方法。