摘要:
There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction.A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof.
摘要:
There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction. A recombinant plasmid containing a vanillate demethylase gene (vanAB genes), benzaldehyde dehydrogenase gene (ligV gene) and protocatechuate 3,4-dioxygenase gene (pcaHG genes); transformants incorporating the plasmid; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of vanillin, vanillic acid, protocatechuic acid or a mixture of two or more thereof.
摘要:
Industrial-scale fermentative production of 3-carboxy-cis,cis-muconic acid from terephthalic acid. Also, a protocatechuate 4,5-ring-cleaving enzyme gene-disrupted strain in which the gene coding for (a) the amino acid sequence set forth in SEQ ID NO: 1 or 3, or (b) the amino acid sequence set forth in SEQ ID NO: 1 or 3 which has a deletion, substitution, addition and/or insertion of one or more amino acids and exhibits protocatechuate 4,5-ring cleavage activity, present in the chromosomal DNA of microbial cells, has been disrupted; recombinant plasmids comprising the Tph gene and protocatechuate 3,4-dioxygenase gene; transformants obtained by introducing the recombinant plasmids into the disrupted strain; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of terephthalic acid.
摘要翻译:从对苯二甲酸工业规模发酵生产3-羧基 - 顺式,顺式 - 粘康酸。 此外,原儿茶素4,5环切割酶基因破坏的菌株,其中编码(a)SEQ ID NO:1或3所示的氨基酸序列的基因,或(b)阐述的氨基酸序列 在具有一个或多个氨基酸的缺失,取代,添加和/或插入并且表现出存在于微生物细胞的染色体DNA中的原儿茶素4,5-环切割活性的SEQ ID NO:1或3已被破坏; 包含Tph基因和原儿茶素3,4-双加氧酶基因的重组质粒; 通过将重组质粒导入破碎的菌株获得的转化体; 以及3-羧基 - 顺式,顺式 - 粘酸和/或3-羧基粘土内酯的制备方法,其特征在于在对苯二甲酸存在下培养转化体。
摘要:
&agr;-Hydroxy-&ggr;-carboxymuconic acid-&egr;-semialdehyde dehydrogenase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant carrying the gene, and processes for producing the enzyme and 2-pyrone-dicarboxylic acid by using the transformant. Use of the transformant can produce &agr;-hydroxy-&ggr;-carboxymuconic acid-&egr;-semialdehyde dehydrogenase in a large amount, thereby permitting industrial production of 2-pyrone-4,6-dicarboxylic acid.
摘要:
&agr;-Hydroxy-&ggr;-carboxymuconic acid-&egr;-semialdehyde dehydrogenase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant carrying the gene, and processes for producing the enzyme and 2-pyrone-dicarboxylic acid by using the transformant. Use of the transformant can produce &agr;-hydroxy-&ggr;-carboxymuconic acid-&egr;-semialdehyde dehydrogenase in a large amount, thereby permitting industrial production of 2-pyrone-4,6-dicarboxylic acid.
摘要:
A latitudinal seal mechanism includes seal jaws 78, fixed cams 75, cam followers 76, a pair of partial cams 88, partial cam followers 78a, rotational shafts 80, coupling members 79, and shaft support portions. The seal jaws 78 are heated, and move circularly such that the seal jaws 78 abut on and move away from tubular film. The fixed cams 75 have shapes that correspond to locuses of movement of the seal jaws 78. The cam followers 76 support the seal jaws 78, and move along the fixed cams 75. The rotational shafts 80 move the cam followers 76 relatively about rotational centers of the rotational shafts 80. The coupling members 79 couple the rotational shafts 80 and the cam followers 76. The shaft support portions rotatably support the rotational shafts 80. The partial cams 88 have a shape that corresponds to a locus of movement of the pair of seal jaws 78 when the pair of seal jaws 78 holds the tubular packaging films. The partial cams 88 make the pair of seal jaws 78 press against each other with the partial cam followers 78a therebetween. The partial cam followers 78a are supported by the pair of seal jaws 78. The partial cam followers 78a engage the corresponding partial cams 88 when the pair of seal jaws 78 holds the tubular packaging film therebetween.
摘要:
A packaging machine has a roll supporting device with a support shaft rotatably supporting a film roll which extends in an axial direction, an elongated bag-forming film being wrapped around the film roll, a bag forming device which pulls out the film from the film roll and forms the film into a shape of a bag, a packaging device for filling the bag-shaped film with articles to be packaged and sealing the film to produce a package, a roll displacing mechanism for displacing the roll supporting device in the axial direction, and a roll position controller for controlling the roll displacing mechanism to adjust the position of the roll supporting device. The bag forming device includes a former for bending the film into a tubular form and a former roller for guiding the film to the former. As a former of a different size is installed, depending on the width of the film, the position of the former roller is changed by a former roller discharging mechanism which includes a first mechanism for supporting the former roller and moving it to a plurality of different positions with respect itself and a second mechanism for moving the first mechanism to a plurality of different positions in the same direction.
摘要:
A poker is provided to a packaging machine which has a former for bending a film into a tubular form and a chute for guiding articles to be packaged into a bag being made below. The poker includes a driver link which is driven to undergo a reciprocating rotary motion around a fixed axis and a generally L-shaped arm member disposed above and behind the former. A base part of the arm member is rotatably connected with a moving end part of the driver link such that the arm member can undergo a reciprocating rotary motion around a mobile axis of rotation which rotates around the stationary axis of rotation. A connecting mechanism is further provided to coordinate these two rotational motions such that the tip of the arm member distal from its mobile axis of rotation will move along a desired trajectory to reach a desired bottom center part of the chute which is likely to become clogged with the articles dropped thereinto. The connecting mechanism may be formed with a cam-following member attached to a part of the arm member and a cam plate with an elongated opening such that this cam-following member is constrained to move along an arcuate path determined by this elongated opening.
摘要:
An elongated web of bag-making material (S) is bent into a tubular form by a former (2), transported along a path (A), is filled with articles (W) and sealed by a transverse sealer (10) transversely to its direction of motion. A pair of seal jaws (20) supported by rotary arms (12) are rotated in mutually opposite directions by a power-communicating mechanism (P) which includes a trajectory-forming mechanism (H) such that the seal jaws (20) will each travel on a generally D-shaped trajectory with a linear section along the path (A) of the bag-making material (S). Cramming devices (50) are adjustably attached to the rotary arms (12) and stroke the tubularly shaped bag-making material (S) from both sides in forward-backward direction (X) without substantially closing the bag being formed such that the articles (W) can be more efficiently crammed with empty spaces among them effectively reduced. Stripping rods (42) are attached to the seal jaws (20) for stripping down the articles being dropped into the bag immediately before the transverse sealing and after the cramming by the cramming devices (50) so as to prevent them from becoming caught between the seal jaws (20).
摘要:
A video signal recording system is provided in which the number of horizontal scanning lines of a high-definition television system video signal is converted to be M times as large as the number of horizontal scanning lines of an ordinary television video signal. The converted video signal is divided into M vertical sections and N horizontal sections so that M.times.N sections of the divided video signal are separately recorded onto M.times.N recording media respectively. In this way, a high-definition television system video signal can be displayed on a plurality of ordinary television receivers without losing the high-definition television system picture quality.