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    5-aminolevulinic acid producing microorganism and process for producing 5-aminolevulinic acid
    5.
    发明授权
    5-aminolevulinic acid producing microorganism and process for producing 5-aminolevulinic acid 失效
    5-氨基乙酰丙酸生产微生物和生产5-氨基乙酰丙酸的方法

    公开(公告)号：US5763235A

    公开(公告)日：1998-06-09

    申请号：US813088

    申请日：1997-03-07

    申请人： Keitaro Watanabe ,  Seiji Nishikawa ,  Tohru Tanaka ,  Yasushi Hotta

    发明人： Keitaro Watanabe ,  Seiji Nishikawa ,  Tohru Tanaka ,  Yasushi Hotta

    IPC分类号： C12P13/00

    CPC分类号： C12P13/005 ,  C12R1/01 ,  C12R1/19 ,  C12R1/38 ,  C12R1/85 ,  Y10S435/822

    摘要： A microorganism that produces 5-aminolevulinic acid, wherein the microorganism has a 5-aminolevulinic acid dehydratase variant having a reduced inhibitor constant for a 5-aminolevulinic acid dehydratase inhibitor. A microorganism that produces 5-aminolevulinic acid, wherein the microorganism is a photosynthetic bacterium that produces 5-aminolevulinic acid without light irradiation. A process for producing 5-aminolevulinic acid comprising the step of culturing a microorganism that produces 5-aminolevulinic acid in a culture medium under at least one condition selected from the group consisting of (a) less than 1 ppm of dissolved oxygen concentration in the culture medium, (b) from -180 to 50 mV of oxidation-reduction potential in the culture medium, and (c from 5.times.10.sup.-9 to (KrM-2.times.10.sup.-8) (mol of O.sub.2 /ml.multidot.min.cndot.cell) of cellular respiration rate, wherein KrM represents the maximum cellular respiration rate when oxygen is supplied in an excess quantity.

    摘要翻译： 一种产生5-氨基乙酰丙酸的微生物，其中微生物具有5-氨基乙酰丙酸脱水酶抑制剂的抑制剂常数降低的5-氨基乙酰丙酸脱水酶变体。 产生5-氨基乙酰丙酸的微生物，其中所述微生物是不照射光而产生5-氨基乙酰丙酸的光合细菌。 一种生产5-氨基乙酰丙酸的方法，包括在选自以下的至少一种条件下培养在培养基中产生5-氨基乙酰丙酸的微生物的步骤：（a）在培养物中小于1ppm的溶解氧浓度 培养基中，（b）培养基中-180至50mV的氧化还原电位，和（c从5×10 -9至（KrM-2×10 -8）（O 2 / ml×Mincell）的细胞呼吸速率， 其中KrM表示当以超量供应氧气时的最大细胞呼吸速率。

    5-aminolevulinic acid producing microorganism and process for producing 5-aminolevulinic acid
    6.
    发明授权
    5-aminolevulinic acid producing microorganism and process for producing 5-aminolevulinic acid 失效
    5-氨基乙酰丙酸生产微生物和生产5-氨基乙酰丙酸的方法

    公开(公告)号：US5733770A

    公开(公告)日：1998-03-31

    申请号：US575818

    申请日：1995-12-20

    申请人： Keitaro Watanabe ,  Seiji Nishikawa ,  Tohru Tanaka ,  Yasushi Hotta

    发明人： Keitaro Watanabe ,  Seiji Nishikawa ,  Tohru Tanaka ,  Yasushi Hotta

    IPC分类号： C12P13/00 ,  C12N1/20

    CPC分类号： C12P13/005 ,  C12R1/01 ,  C12R1/19 ,  C12R1/38 ,  C12R1/85 ,  Y10S435/822

    摘要： A microorganism that produces 5-aminolevulinic acid, wherein the microorganism has a 5-aminolevulinic acid dehydratase variant having a reduced inhibitor constant for a 5-aminolevulinic acid dehydratate inhibitor. A microorganism that produces 5-aminolevulinic acid, wherein the microorganism is a photosynthetic bacterium that produces 5-aminolevulinic acid without light irradiation. A progess for producing 5-aminolevulinic acid comprising the step of culturing a microorganism that produces 5-aminolevulinic acid in a culture medium under at least one condition selected from the group consisting of (a) less than 1 ppm of dissolved oxygen concentration in the culture medium, (b) from -180 to 50 mV of oxidation-reduction potential in the culture medium, and (c) from 5.times.10.sup.-9 to (KrM-2.times.10.sup.-8) (mol of O.sub.2 /ml.multidot.min.multidot.cell) of cellular respiration rate, wherein KrM represents the maximum cellular respiration rate when oxygen is supplied in an excess quantity.

    摘要翻译： 一种产生5-氨基乙酰丙酸的微生物，其中微生物具有5-氨基乙酰丙酸脱水抑制剂的抑制剂常数降低的5-氨基乙酰丙酸脱水酶变体。 产生5-氨基乙酰丙酸的微生物，其中所述微生物是不照射光而产生5-氨基乙酰丙酸的光合细菌。 一种用于生产5-氨基乙酰丙酸的方法，包括在选自以下的至少一种条件下培养在培养基中产生5-氨基乙酰丙酸的微生物的步骤：（a）在培养物中小于1ppm的溶解氧浓度 培养基中，（b）在培养基中的-180至50mV的氧化还原电位，和（c）5×10 -9至（KrM-2×10 -8）（摩尔的O 2 / ml×Min×细胞）的细胞呼吸速率，其中 当以超量供给氧气时，KrM表示最大的细胞呼吸速率。

    Microorganisms producing 5-aminolevulinic acid and processes for producing 5-aminolevulinic acid by using the same
    7.
    发明授权
    Microorganisms producing 5-aminolevulinic acid and processes for producing 5-aminolevulinic acid by using the same 有权
    生产5-氨基乙酰丙酸的微生物及使用该5-氨基乙酰丙酸的方法

    公开(公告)号：US06342377B1

    公开(公告)日：2002-01-29

    申请号：US09424671

    申请日：2000-02-28

    申请人： Seiji Nishikawa ,  Tohru Tanaka ,  Tomomi Kaminaga ,  Kikuo Watanabe ,  Nobuya Miyachi ,  Keitaro Watanabe ,  Yasushi Hotta

    发明人： Seiji Nishikawa ,  Tohru Tanaka ,  Tomomi Kaminaga ,  Kikuo Watanabe ,  Nobuya Miyachi ,  Keitaro Watanabe ,  Yasushi Hotta

    IPC分类号： C12P1300

    CPC分类号： C12N9/1029 ,  C12P13/005

    摘要： Microorganisms producing 5-aminolevulinic acid which show a 5-aminolevulinic acid synthetase activity of 2 to 7 (nmol/min/mg protein) under aerobic culture conditions with a dissolved oxygen concentration of 0.70 to 6.60 ppm; a process for producing 5-aminolevulinic acid characterized by culturing one or more microorganisms and harvesting 5-aminolevulinic acid from the obtained culture; a process for producing 5-aminolevulinic acid characterized by culturing 5-aminolevulinic acid-producing microorganisms in a medium containing 5 to 500 &mgr;M of iron components; and a method for culturing 5-aminolevulinic acid-producing microorganisms.

    摘要翻译： 产生5-氨基乙酰丙酸的微生物，其在需氧培养条件下，溶解氧浓度为0.70至6.60ppm，显示5-7个氨基乙酰丙酸合成酶活性为2至7（nmol / min / mg蛋白）; 一种生产5-氨基乙酰丙酸的方法，其特征在于培养一种或多种微生物并从所得培养物中收获5-氨基乙酰丙酸; 一种生产5-氨基乙酰丙酸的方法，其特征在于在含有5-500微米铁成分的培养基中培养5-氨基乙酰丙酸生产微生物; 以及培养5-氨基乙酰丙酸生产微生物的方法。

    Method for improving pigmentation of rind of apple
    8.
    发明授权
    Method for improving pigmentation of rind of apple 失效
    改善苹果皮肤色素沉着的方法

    公开(公告)号：US5318788A

    公开(公告)日：1994-06-07

    申请号：US151264

    申请日：1993-11-12

    申请人： Kiyoshi Yokota ,  Tohru Tanaka ,  Yasushi Hotta

    发明人： Kiyoshi Yokota ,  Tohru Tanaka ,  Yasushi Hotta

    IPC分类号： A01G7/00 ,  A01G7/06 ,  A01N37/44 ,  A23L1/272

    CPC分类号： A01N37/44 ,  A01G7/06

    摘要： A method for improving pigmentation of the rind of anthocyanin-containing apples comprising treating fruit-bearing apple trees or apples before or after harvesting with an effective amount of an agent which comprises 5-aminolevulinic acid, a salt thereof or a mixture thereof as an active component.

    摘要翻译： 一种用于改善含花青素的苹果皮的色素沉着的方法，包括用有效量的包含5-氨基乙酰丙酸，其盐或其混合物作为活性物质的试剂在收获之前或之后处理果实的苹果树或苹果 零件。

    Method for promoting plant growth using 5-aminolevulinic acid or a salt thereof
    9.
    发明授权
    Method for promoting plant growth using 5-aminolevulinic acid or a salt thereof 失效
    使用5-氨基乙酰丙酸或其盐促进植物生长的方法

    公开(公告)号：US5298482A

    公开(公告)日：1994-03-29

    申请号：US881705

    申请日：1992-05-12

    申请人： Tohru Tanaka ,  Kiyoshi Takahashi ,  Yasushi Hotta ,  Yasutomo Takeuchi ,  Makoto Konnai

    发明人： Tohru Tanaka ,  Kiyoshi Takahashi ,  Yasushi Hotta ,  Yasutomo Takeuchi ,  Makoto Konnai

    IPC分类号： A01N37/44

    CPC分类号： A01N37/44

    摘要： A method for treating plants with a plant growth promoter comprising 5-aminolevulinic acid or a salt thereof as an active ingredient. This treatment method is effective in enhancing the photosynthetic activity of a plant, enhancing its ability to absorb CO.sub.2, suppressing its respiration, increasing its chlorophyll content and promoting its growth. As a result, the treatment greatly contributes to the promotion of rooting, the reduction of lodging, an increase in yield, an improvement in cold resistance, maintenance of freshness, an improvement and maintenance of green color, the growth of good seedlings, the promotion of organs, an increase in tillers, a shortening of the time required for growth, a relief of chemical damage and an increase in the rooting ratio in, for example, cutting.

    摘要翻译： 用植物生长促进剂处理植物的方法，其包含5-氨基乙酰丙酸或其盐作为活性成分。 这种处理方法对于提高植物的光合活性，提高其吸收CO2的能力，抑制其呼吸，增加叶绿素含量，促进其生长有效。 因此，处理有助于促进生根，减少倒伏，提高产量，提高耐寒性，保持新鲜度，改善和保持绿色，良好的幼苗生长，促进 的器官，分蘖的增加，生长所需时间的缩短，化学损害的缓解以及例如切割中生根比的增加。

    Methods for reducing nitrate nitrogen and oxalic acids contents nin plants
    10.
    发明授权
    Methods for reducing nitrate nitrogen and oxalic acids contents nin plants 失效
    减少植物硝酸盐氮和草酸含量的方法

    公开(公告)号：US5489572A

    公开(公告)日：1996-02-06

    申请号：US292024

    申请日：1994-08-18

    申请人： Ryuji Yoshida ,  Tohru Tanaka ,  Yasushi Hotta

    发明人： Ryuji Yoshida ,  Tohru Tanaka ,  Yasushi Hotta

    IPC分类号： A01N37/44

    CPC分类号： A01N37/44 ,  Y02P60/218

    摘要： Methods for reducing nitrate nitrogen and oxalic acid contents in plants, each of which comprises applying 5-aminolevulinic acid, a salt thereof or a mixture thereof. Nitrate nitrogen and oxalic acid contents in plants having high nitrate nitrogen and oxalic acid contents can be reduced effectively and easily.

    摘要翻译： 减少植物中硝酸盐氮和草酸含量的方法，每种方法包括施用5-氨基乙酰丙酸，其盐或其混合物。 硝酸盐氮和草酸含量高的植物中的氮和草酸含量可以有效降低。