公开(公告)号：US20090081371A1

公开(公告)日：2009-03-26

申请号：US12212942

申请日：2008-09-18

申请人： Koichi Minami ,  Morihito Ikeda ,  Yohsuke Takeuchi

发明人： Koichi Minami ,  Morihito Ikeda ,  Yohsuke Takeuchi

IPC分类号： B05D3/00 ,  C12M1/34

CPC分类号： G01N33/54353 ,  C07K17/06 ,  C07K17/14 ,  G01N33/54386 ,  G01N33/54393

摘要： A substrate includes: a base material; and chelators which are three dimensionally covalently bound to the base material at a density of 7.8×1015/mm3 or greater and 4.5×1017/mm3 or less, or two dimensionally covalently bound to the base material at a planar density of 2.0×1011/mm2 or greater and 1.0×1013/mm2 or less. Metal ions are coordinately bound to the chelators, and a bioactive substance having histidine tags are coordinately bound to the metal ions. A blocking agent having ligand sets are coordinately bound to the metal ions to which the bioactive substance is not coordinately bound.

摘要翻译： 基板包括：基材; 和以7.8×10 15 / mm 3以上且4.5×10 17 / mm 3以下的密度三维共价结合于基材的螯合剂，或者以2.0×10 11 / mm 2以上的平面密度二维共价结合到基材上 和1.0×10 13 / mm 2以下。 金属离子与螯合剂配位结合，具有组氨酸标签的生物活性物质与金属离子配位结合。 具有配体组的封闭剂与生物活性物质不配位结合的金属离子配位结合。

公开(公告)号：US08529766B2

公开(公告)日：2013-09-10

申请号：US12915973

申请日：2010-10-29

申请人： Koichi Minami ,  Morihito Ikeda ,  Hiroshi Arakatsu

发明人： Koichi Minami ,  Morihito Ikeda ,  Hiroshi Arakatsu

IPC分类号： B01D11/00 ,  B01D61/00 ,  B01D3/00 ,  C02F1/44 ,  C02F1/00

CPC分类号： C02F1/44 ,  B01D61/00 ,  B01D61/002 ,  B01D61/145 ,  B01D61/243 ,  B01D61/364 ,  B01D61/422 ,  B01D61/58 ,  B01D2311/2626 ,  C02F1/444 ,  C02F1/445 ,  C02F1/4693 ,  C02F2301/08 ,  C02F2303/16

摘要： To provide a water purifier, containing: a diluting unit configured to bring targeted water for purification into contact with an aqueous solution containing a volatile solute and a polymer via a semi-permeable membrane so as to separate water from the targeted water by the semi-permeable membrane, and configured to dilute the aqueous solution with the separated water; a separating unit configured to separate the volatile solute and the polymer from the diluted aqueous solution obtained by the diluting unit so as to obtain purified water; and a dissolving unit configured to return the separated volatile solute by the separating unit to the aqueous solution containing the polymer, and to allow the separated volatile solute to dissolve in the aqueous solution containing the polymer.

摘要翻译： 本发明提供一种净水器，其特征在于，含有：稀释单元，其配置为使净化物与含有挥发性溶质的水溶液和聚合物通过半透膜接触，以通过半透过膜将水与目标水分离， 并且被配置为用分离的水稀释水溶液; 分离单元，被配置为从由稀释单元获得的稀释水溶液中分离挥发性溶质和聚合物以获得纯化水; 以及溶解单元，其被配置为将分离单元中分离的挥发性溶质返回到含有聚合物的水溶液中，并且使分离的挥发性溶质溶解在含有聚合物的水溶液中。

公开(公告)号：US20090062515A1

公开(公告)日：2009-03-05

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/00

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质通过10mmol / L至1mol / L咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US08557194B2

公开(公告)日：2013-10-15

申请号：US13188637

申请日：2011-07-22

申请人： Koichi Minami ,  Yohsuke Takeuchi

发明人： Koichi Minami ,  Yohsuke Takeuchi

IPC分类号： B05D5/00 ,  B05D1/36

CPC分类号： G01N33/54353 ,  C07K1/22 ,  C07K2319/21 ,  G01N33/54386 ,  G01N33/54393

摘要： A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×1016 pieces/mm3 to 3.3×1018 pieces/mm3. The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

摘要翻译： 载体包括已经结合在基板的表面上的基板，聚合物膜和已经与聚合物膜结合的配体。 该配体以1.0×10 16个/ mm 3〜3.3×10 18个/ mm 3的范围内的密度与聚合物膜结合。 通过一种方法制备载体，其包括以下步骤：使聚合物膜结合在基板上，并使配体与聚合物膜结合，使配体与聚合物膜结合的步骤在 有机溶剂。

公开(公告)号：US20110274846A1

公开(公告)日：2011-11-10

申请号：US13188637

申请日：2011-07-22

申请人： Koichi Minami ,  Yohsuke Takeuchi

发明人： Koichi Minami ,  Yohsuke Takeuchi

IPC分类号： B05D5/00 ,  B05D1/36

CPC分类号： G01N33/54353 ,  C07K1/22 ,  C07K2319/21 ,  G01N33/54386 ,  G01N33/54393

摘要： A carrier comprises a base plate, a polymeric film, which has been bound on a surface of the base plate, and a ligand, which has been bound with the polymeric film. The ligand has been bound with the polymeric film at a density falling within the range of 1.0×1016 pieces/mm3 to 3.3×1018 pieces/mm3. The carrier is produced with a process, comprising the steps of: causing the polymeric film to bind on the base plate, and causing the ligand to bind with the polymeric film, the step of causing the ligand to bind with the polymeric film being performed in an organic solvent.

摘要翻译： 载体包括已经结合在基板的表面上的基板，聚合物膜和已经与聚合物膜结合的配体。 该配体以1.0×10 16个/ mm 3至3.3×10 18个/ mm 3的范围内的密度与聚合物膜结合。 通过一种方法制备载体，其包括以下步骤：使聚合物膜结合在基板上，并使配体与聚合物膜结合，使配体与聚合物膜结合的步骤在 有机溶剂。

公开(公告)号：US07928191B2

公开(公告)日：2011-04-19

申请号：US12201594

申请日：2008-08-29

申请人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

发明人： Koichi Minami ,  Yohsuke Takeuchi ,  Taisei Nishimi

IPC分类号： C07K1/16

CPC分类号： G01N33/54353 ,  C07K1/22 ,  G01N33/54386 ,  G01N33/54393

摘要： A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

摘要翻译： 净化生物活性物质的方法包括以下步骤：使具有组氨酸单元的生物活性物质与底物接触的介质，物理上附着于基材表面的配体和Cu（II）或Fe（II） 与配体共价键合的金属离子; 使生物活性物质通过组氨酸单元与金属离子共价键合; 并用介质的体积为60倍量的1nmol / L至10mmol / L的咪唑衍生物溶液洗涤培养基。 在金属离子为Cu（II）的情况下，通过组氨酸单元与Cu（II）共价键合的生物活性物质由10mmol / L至1mol / L的咪唑衍生物溶液中的一种回收， 0.5 mmol / L至5 mol / L EDTA溶液。

公开(公告)号：US20110237462A1

公开(公告)日：2011-09-29

申请号：US13132834

申请日：2009-12-04

申请人： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

发明人： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

IPC分类号： C40B40/10 ,  C40B50/14

CPC分类号： G01N33/54353

摘要： An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein antibody fragments of each set include at least two types of separate antibody fragments, the at least two types of separate antibody fragments include at least one type of labeled antibody fragment having a labeled site labeled with a luminescent substance and at least one type of acceptor antibody fragment having an acceptor site for accepting emission from the labeled antibody fragment, and the at least one type of labeled antibody fragment and the at least one type of acceptor antibody fragment are capable of cooperatively recognizing one type of antigen in combination and are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

摘要翻译： 抗体片段固定的底物包括底物和至少一组抗体片段，其中每组的抗体片段包括至少两种类型的分离的抗体片段，所述至少两种类型的分离的抗体片段包括至少一种类型的 具有用发光物质标记的标记位点的标记抗体片段和至少一种具有用于接受来自标记抗体片段的发射的受体位点的受体抗体片段，以及至少一种类型的标记抗体片段和至少一种类型 的受体抗体片段能够组合地协同识别一种类型的抗原，并以允许一组中的每个抗体片段结合相同抗原的位置关系独立地固定在底物上。

公开(公告)号：US4382597A

公开(公告)日：1983-05-10

申请号：US187567

申请日：1980-09-15

申请人： Koichi Minami

发明人： Koichi Minami

IPC分类号： A63F7/02 ,  A63F7/06 ,  A63F7/00

CPC分类号： A63F7/2409 ,  A63F7/025 ,  A63F7/066 ,  A63F2250/04 ,  A63F2250/0407

摘要： A pinball game is contained within a housing which includes a liquid filled chamber. At least one ball is located within the liquid within the chamber. The ball has a specific gravity heavier than the liquid such that it tends to sink within the liquid but it is light enough to be conveyed upwardly by currents within the liquid. A flipper is located within the chamber and is capable of interacting with the ball to propel it within the confines of the chamber. The flipper is controlled by an actuator member located on the housing and operatively connected to the flipper to move the flipper. At least one goal receptacle is positioned within the chamber and the ball is capable of being located or deposited in the goal receptacle. It is the object to deposit the ball in the goal receptacle by manipulating the ball with the flippers and/or currents of liquid.

摘要翻译： 弹珠游戏包含在包括充满液体的腔室的壳体内。 至少一个球位于腔室内的液体内。 球具有比液体更重的比重，使得其倾向于在液体内沉降，但是其足够轻以通过液体内的电流被向上输送。 导板位于腔室内，能够与球体相互作用，以将其推进腔室的范围内。 导板由位于壳体上的致动器构件控制，并且可操作地连接到导板以移动导板。 至少一个目标容器定位在腔室内，并且球能够定位或沉积在球门座中。 目标是通过用脚蹼和/或液体流动操纵球，将球沉积在球门座中。

公开(公告)号：US09487592B2

公开(公告)日：2016-11-08

申请号：US13125478

申请日：2009-10-23

申请人： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

发明人： Koichi Minami ,  Hirohiko Tsuzuki ,  Hiroshi Ueda ,  Masaki Ihara

IPC分类号： C07K16/18 ,  C07K16/40

CPC分类号： C07K16/40 ,  C07K16/18 ,  C07K2317/56 ,  Y10T428/269

摘要： An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein the antibody fragments of each set includes at least two types of separate antibody fragments that are capable of recognizing one type of antigen and that are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

摘要翻译： 抗体片段固定底物包括底物和至少一组抗体片段，其中每组的抗体片段包含能够识别一种类型的抗原并且独立地固定在其上的至少两种类型的分离的抗体片段 位置关系的底物允许一组中的每个抗体片段与相同的抗原结合。

公开(公告)号：US20100290954A1

公开(公告)日：2010-11-18

申请号：US12744311

申请日：2008-11-21

申请人： Koichi Minami ,  Hirohiko Tsuzuki

发明人： Koichi Minami ,  Hirohiko Tsuzuki

IPC分类号： G01N21/64 ,  G01N33/543

CPC分类号： G01N33/542 ,  G01N21/6428

摘要： A first reactant, which is provided with a reaction site for specific binding with an analyte, and a fluorescent label site, and a second reactant, which is provided with a reaction site for specific binding with the analyte, and a fluorescence recognition site for recognizing fluorescence produced by the fluorescent label site of the first reactant, are respectively fixed onto a support such that the first reactant and the second reactant have a positional relationship adapted for the binding with the analyte.

摘要翻译： 提供有用于与分析物特异性结合的反应位点的第一反应物和荧光标记位点，以及第二反应物，其具有用于与分析物特异性结合的反应位点和用于识别的荧光识别位点 由第一反应物的荧光标记位点产生的荧光分别固定在载体上，使得第一反应物和第二反应物具有适于与分析物结合的位置关系。