公开(公告)号：US08492084B2

公开(公告)日：2013-07-23

申请号：US12162008

申请日：2007-01-24

申请人： Koji Sode ,  Kazunori Ikebukuro ,  Hiroki Yamazaki

发明人： Koji Sode ,  Kazunori Ikebukuro ,  Hiroki Yamazaki

IPC分类号： C12Q1/68

CPC分类号： G01N33/5308 ,  C12Q1/6825 ,  G01N33/58 ,  C12Q2565/628 ,  C12Q2565/518 ,  C12Q2525/205

摘要： Disclosed are a method for assaying a target substance in a sample and an apparatus for the method. The method can specifically assay the target substance in the sample without using any antibody against the target substance. The assaying method includes simultaneously or successively bringing a labeled aptamer, the target substance in the sample and a solid phase into contact together, and then measuring the label of the aptamer which has not been bound on the solid phase. The labeled aptamer has a property of binding to the target substance. The solid phase carries an oligonucleotide immobilized on it in an excess amount relative to the target substance. The oligonucleotide is hybridizable with the labeled aptamer when the labeled aptamer is in a state that it is not bound to the target substance, but is not hybridizable with the labeled aptamer when the labeled aptamer is in a state that it is bound to the target substance.

摘要翻译： 公开了用于测定样品中的目标物质的方法和该方法的装置。 该方法可以特异性地测定样品中的目标物质，而不使用针对目标物质的任何抗体。 测定方法包括同时或连续地将标记的适体，样品中的目标物质和固相接触在一起，然后测量未结合在固相上的适体的标记。 标记的适体具有与靶物质结合的性质。 固相携带相对于目标物质过量固定在其上的寡核苷酸。 当标记的适体处于与靶物质不结合的状态下，当标记的适体处于与靶物质结合的状态时，寡核苷酸可与标记的适配子杂交，但与标记的适体不可杂交 。

公开(公告)号：US20090305254A1

公开(公告)日：2009-12-10

申请号：US12162008

申请日：2007-01-24

申请人： Koji Sode ,  Kazunori Ikebukuro ,  Hiroki Yamazaki

发明人： Koji Sode ,  Kazunori Ikebukuro ,  Hiroki Yamazaki

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： G01N33/5308 ,  C12Q1/6825 ,  G01N33/58 ,  C12Q2565/628 ,  C12Q2565/518 ,  C12Q2525/205

摘要： Disclosed are a method for assaying a target substance in a sample and an apparatus for the method. The method can specifically assay the target substance in the sample without using any antibody against the target substance. The assaying method includes simultaneously or successively bringing a labeled aptamer, the target substance in the sample and a solid phase into contact together, and then measuring the label of the aptamer which has not been bound on the solid phase. The labeled aptamer has a property of binding to the target substance. The solid phase carries an oligonucleotide immobilized on it in an excess amount relative to the target substance. The oligonucleotide is hybridizable with the labeled aptamer when the labeled aptamer is in a state that it is not bound to the target substance, but is not hybridizable with the labeled aptamer when the labeled aptamer is in a state that it is bound to the target substance.

摘要翻译： 公开了用于测定样品中的目标物质的方法和该方法的装置。 该方法可以特异性地测定样品中的目标物质，而不使用针对目标物质的任何抗体。 测定方法包括同时或连续地将标记的适体，样品中的目标物质和固相接触在一起，然后测量未结合在固相上的适体的标记。 标记的适体具有与靶物质结合的性质。 固相携带相对于目标物质过量固定在其上的寡核苷酸。 当标记的适体处于与靶物质不结合的状态下，当标记的适体处于与靶物质结合的状态时，寡核苷酸可与标记的适配子杂交，但与标记的适体不可杂交 。

公开(公告)号：US08143004B2

公开(公告)日：2012-03-27

申请号：US12443053

申请日：2007-09-27

申请人： Kazunori Ikebukuro ,  Koji Sode

发明人： Kazunori Ikebukuro ,  Koji Sode

IPC分类号： C12Q1/68

CPC分类号： G01N33/5308

摘要： A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.

摘要翻译： 一种测试物质的方法，其中可以特异性地测量样品中的测试物质，而不使用针对测试物质的抗体; 用于其的适体分子; 并且公开了用于产生适配体的方法。 当与被检物质结合时能够与寡核苷酸杂交，但是当不与被检物质结合时，不能与寡核苷酸杂交的适体。 使适体与样品接触，使与测试物质结合的适配体与与适体杂交的固定化寡核苷酸接触，将适体结合固相，随后测定固定在 固相。

公开(公告)号：US20140072986A1

公开(公告)日：2014-03-13

申请号：US14000190

申请日：2012-02-20

申请人： Kazunori Ikebukuro ,  Kaori Tsukakoshi ,  Koji Sode

发明人： Kazunori Ikebukuro ,  Kaori Tsukakoshi ,  Koji Sode

IPC分类号： C12N15/115 ,  G01N33/68

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2310/50 ,  G01N33/6896

摘要： An aptamer having a G-quartet structure and being at least one selected from the group consisting of the following polynucleotides, the aptamer having a binding ability to an amyloid protein oligomer: (1) a polynucleotide comprising a base sequence represented by any one of SEQ ID NO:1 to SEQ ID NO:18; (2) a polynucleotide comprising a base sequence that includes at least four sets of at least two consecutive guanosine nucleotides and in which one or several bases has been deleted, substituted, or added in a base sequence represented by any one of SEQ ID NO:18; and (3) a polynucleotide that is a multimer including the polynucleotide of (1) or (2) as a structural unit.

摘要翻译： 具有G-四重结构并且选自下列多核苷酸中的至少一种的适体，所述适配体具有与淀粉样蛋白寡聚物的结合能力：（1）包含由SEQ ID NO： SEQ ID NO：1至SEQ ID NO：18; （2）包含碱基序列的多核苷酸，其包含至少两组至少两个连续的鸟苷核苷酸，并且其中一个或几个碱基已被缺失，取代或加入到由SEQ ID NO： 18; 和（3）多核苷酸，其是包含作为结构单元的（1）或（2）的多核苷酸的多聚体。

公开(公告)号：US20090042218A1

公开(公告)日：2009-02-12

申请号：US11663345

申请日：2005-09-21

申请人： Kazunori Ikebukuro ,  Koji Sode ,  Akira Umezawa ,  Kyoko Umezawa ,  Hironobu Yamamoto

发明人： Kazunori Ikebukuro ,  Koji Sode ,  Akira Umezawa ,  Kyoko Umezawa ,  Hironobu Yamamoto

IPC分类号： G01N33/573 ,  C12N9/50 ,  C12N9/74

CPC分类号： G01N33/535 ,  C12Q1/56 ,  G01N21/45 ,  G01N21/553 ,  G01N33/581 ,  G01N33/86 ,  G01N2333/75 ,  G01N2333/974

摘要： The present invention relates to a labeling enzyme and a method of detecting and/or quantifying a target substance using this labeling enzyme. The present invention provides a labeling enzyme that catalyzes a reaction of gelling a substrate. By measuring changes in physical properties such as the film thickness and/or refractive index of a film produced by the gelling reaction catalyzed by the labeling enzyme of the present invention, it is possible to quickly and highly sensitively detect and/or quantify a target substance while minimizing the effects of coexisting substances.

摘要翻译： 本发明涉及使用该标记酶的标记酶和检测和/或定量目标物质的方法。 本发明提供一种催化凝胶化基质的反应的标记酶。 通过测量通过本发明的标记酶催化的胶凝反应产生的膜的物理性质的变化，例如膜的厚度和/或折射率，可以迅速和高度灵敏地检测和/或定量目标物质 同时尽量减少共存物质的影响。

公开(公告)号：US09238816B2

公开(公告)日：2016-01-19

申请号：US14000190

申请日：2012-02-20

申请人： Kazunori Ikebukuro ,  Kaori Tsukakoshi ,  Koji Sode

发明人： Kazunori Ikebukuro ,  Kaori Tsukakoshi ,  Koji Sode

IPC分类号： C12N15/115 ,  G01N33/68

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12N2310/50 ,  G01N33/6896

摘要： An aptamer having a G-quartet structure and being at least one selected from the group consisting of the following polynucleotides, the aptamer having a binding ability to an amyloid protein oligomer: (1) a polynucleotide comprising a base sequence represented by any one of SEQ ID NO:1 to SEQ ID NO:18; (2) a polynucleotide comprising a base sequence that includes at least four sets of at least two consecutive guanosine nucleotides and in which one or several bases has been deleted, substituted, or added in a base sequence represented by any one of SEQ ID NO:18; and (3) a polynucleotide that is a multimer including the polynucleotide of (1) or (2) as a structural unit.

摘要翻译： 具有G-四重结构并且选自下列多核苷酸中的至少一种的适体，所述适配体具有与淀粉样蛋白寡聚物的结合能力：（1）包含由SEQ ID NO： SEQ ID NO：1至SEQ ID NO：18; （2）包含碱基序列的多核苷酸，其包含至少两组至少两个连续的鸟苷核苷酸，并且其中一个或几个碱基已被缺失，取代或加入到由SEQ ID NO： 18; 和（3）多核苷酸，其是包含作为结构单元的（1）或（2）的多核苷酸的多聚体。

公开(公告)号：US20100021899A1

公开(公告)日：2010-01-28

申请号：US12443053

申请日：2007-09-27

申请人： Kazunori Ikebukuro ,  Koji Sode

发明人： Kazunori Ikebukuro ,  Koji Sode

IPC分类号： C12Q1/68 ,  C12N15/115

CPC分类号： G01N33/5308

摘要： A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.

摘要翻译： 一种测试物质的方法，其中可以特异性地测量样品中的测试物质，而不使用针对测试物质的抗体; 用于其的适体分子; 并且公开了用于产生适配体的方法。 当与被检物质结合时能够与寡核苷酸杂交，但是当不与被检物质结合时，不能与寡核苷酸杂交的适体。 使适体与样品接触，使与测试物质结合的适配体与与适体杂交的固定化寡核苷酸接触，将适体结合固相，随后测定固定在 固相。

公开(公告)号：US08247197B2

公开(公告)日：2012-08-21

申请号：US12874202

申请日：2010-09-01

申请人： Koji Sode ,  Kazunori Ikebukuro

发明人： Koji Sode ,  Kazunori Ikebukuro

IPC分类号： C12P19/34

CPC分类号： G01N33/54393 ,  C12Q1/6816 ,  G01N33/543 ,  C12Q2525/161 ,  C12Q2525/205 ,  C12Q2565/107

摘要： An aptamer-probe complex for detecting the presence of a target molecule is disclosed. The complex of the present invention contains an aptamer moiety which is able to bind to an indicator protein and change the properties of the indicator protein, and a probe moiety which is able to bind to a target molecule, wherein the aptamer moiety and the probe moiety are combined in such a manner that the binding mode between the aptamer moiety and the indicator protein changes when the probe moiety binds to the target molecule. A target molecule can be detected with combination of an aptamer which binds to a certain protein, and a probe which binds to the target molecule, utilizing the properties of that protein as an indicator.

摘要翻译： 公开了一种用于检测靶分子存在的适体 - 探针复合物。 本发明的复合物包含能够结合指示蛋白并改变指示蛋白的性质的适体部分，以及能够结合靶分子的探针部分，其中适体部分和探针部分 以使得当探针部分结合到靶分子时，适体部分和指示蛋白之间的结合模式发生变化的方式组合。 可以通过结合某种蛋白质的适配体的组合和结合靶分子的探针，利用该蛋白质的特性作为指标来检测靶分子。

公开(公告)号：US08721853B2

公开(公告)日：2014-05-13

申请号：US13365384

申请日：2012-02-03

申请人： Kazunori Ikebukuro ,  Koji Sode

发明人： Kazunori Ikebukuro ,  Koji Sode

IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12N9/00 ,  C12N9/06 ,  C07H21/04

CPC分类号： C12Q1/26 ,  C12Q1/004 ,  G01N33/723

摘要： In one form, a fructosyl peptidyl oxidase derived from a budding yeast Phaeosphaeria nodorum for assaying a glycated protein in a sample is provided. The fructosyl peptidyl oxidase has higher activity toward fructosyl valine as well as fructosyl valyl histidine, and may be useful in assaying HbA1c with higher sensitivity and specificity. Still, other forms include unique methods, techniques, systems and devices involving a fructosyl peptidyl oxidase.

摘要翻译： 在一种形式中，提供了源自出芽的酵母菌（Phaeosphaeria nodorum）的果糖基肽基氧化酶，用于测定样品中的糖化蛋白质。 果糖基肽基氧化酶对果糖基缬氨酸以及果糖基缬氨酰基组氨酸具有较高的活性，可用于检测具有更高灵敏度和特异性的HbA1c。 然而，其他形式包括涉及果糖基肽基氧化酶的独特方法，技术，系统和装置。

公开(公告)号：US07807351B2

公开(公告)日：2010-10-05

申请号：US10580044

申请日：2004-11-22

申请人： Koji Sode ,  Kazunori Ikebukuro

发明人： Koji Sode ,  Kazunori Ikebukuro

IPC分类号： C12Q1/68 ,  C12N9/74

CPC分类号： G01N33/54393 ,  C12Q1/6816 ,  G01N33/543 ,  C12Q2525/161 ,  C12Q2525/205 ,  C12Q2565/107

摘要： An aptamer-probe complex for detecting the presence of a target molecule is disclosed. The complex of the present invention contains an aptamer moiety which is able to bind to an indicator protein and change the properties of the indicator protein, and a probe moiety which is able to bind to a target molecule, wherein the aptamer moiety and the probe moiety are combined in such a manner that the binding mode between the aptamer moiety and the indicator protein changes when the probe moiety binds to the target molecule. A target molecule can be detected with combination of an aptamer which binds to a certain protein, and a probe which binds to the target molecule, utilizing the properties of that protein as an indicator.

摘要翻译： 公开了一种用于检测靶分子存在的适体 - 探针复合物。 本发明的复合物包含能够结合指示蛋白并改变指示蛋白的性质的适体部分，以及能够结合靶分子的探针部分，其中适体部分和探针部分 以使得当探针部分结合到靶分子时，适体部分和指示蛋白之间的结合模式发生变化的方式组合。 可以通过结合某种蛋白质的适配体的组合和结合靶分子的探针，利用该蛋白质的特性作为指标，检测靶分子。