公开(公告)号：US4969046A

公开(公告)日：1990-11-06

申请号：US358647

申请日：1989-05-30

申请人： Kouichi Sugimoto ,  Nobuyuki Kurosawa ,  Yoshitomo Ueno

发明人： Kouichi Sugimoto ,  Nobuyuki Kurosawa ,  Yoshitomo Ueno

IPC分类号： H04N5/63 ,  H04N5/64

CPC分类号： H04N5/63 ,  H04N5/64

摘要： A modular television apparatus including a video display module, a tuner module, and a power supply module, the modules being releasably combinable in a variety of combinations. The tuner module is provided with a switch for controlling a supply of power supply voltage from the power supply module to the remaining modules. The tuner module has a casing including an elongated recess into which a telescopic antenna can be stored, the recess conforming to the shape of the antenna. Switches are disposed within the recess, some of the switches having an exposed surface that conforms to the contour of the recess. The video display module is provided with a circuit for controlling the supply of power to other circuits according to detection of input of audio/video signals to the display modules.

摘要翻译： 一种包括视频显示模块，调谐器模块和电源模块的模块化电视设备，所述模块以各种组合可释放地组合。 调谐器模块设置有用于控制从电源模块向其余模块供电的电源的开关。 调谐器模块具有包括细长凹槽的壳体，可以存储伸缩天线，凹槽符合天线的形状。 开关设置在凹部内，一些开关具有符合凹槽轮廓的暴露表面。 视频显示模块设置有用于根据对显示模块的音频/视频信号的输入的检测来控制向其他电路的电力供应的电路。

公开(公告)号：US09015508B2

公开(公告)日：2015-04-21

申请号：US13316474

申请日：2011-12-10

申请人： Satoshi Yoshida ,  Nobuyuki Kurosawa ,  Kenta Sasaki

发明人： Satoshi Yoshida ,  Nobuyuki Kurosawa ,  Kenta Sasaki

IPC分类号： G06F1/32 ,  G06F1/04 ,  G06F15/78

CPC分类号： G06F1/3243 ,  G06F1/04 ,  G06F15/78 ,  H03L7/08 ,  H03L7/14 ,  H03L2207/08

摘要： Even after power-down, distinction between a transition from a PLL normal-oscillation state and a transition from a PLL self-oscillation is allowed.A semiconductor device includes a first region which, after having transited from a power-supply state to a power-down state, returns to the power-supply state again, a second region which holds a power source voltage regardless of power-down of the first region, and an oscillator which generates a first clock signal supplied to the first region. The first region includes a PLL circuit. The second region includes an information holding unit capable of holding information which can distinguish whether the operation mode of the PLL circuit is a PLL normal-oscillation mode or a PLL self-oscillation mode, and determines the operation mode of the PLL circuit when the first region has returned from the power-down state to the power-supply state, according to the information held in the information holding unit.

摘要翻译： 即使在掉电之后，允许从PLL正常振荡状态的转变与来自PLL自振荡的转变之间的区别。 半导体器件包括：在从电源状态转换到掉电状态之后再次返回到电源状态的第一区域，第一区域，其保持电源电压，而与第 第一区域和产生提供给第一区域的第一时钟信号的振荡器。 第一区域包括PLL电路。 第二区域包括信息保持单元，其能够保存可以区分PLL电路的操作模式是PLL正常振荡模式还是PLL自振模式的信息，并且当第一个 根据保存在信息保持单元中的信息，区域从断电状态返回到电源状态。

公开(公告)号：US20140031528A1

公开(公告)日：2014-01-30

申请号：US14008567

申请日：2012-03-28

申请人： Nobuyuki Kurosawa ,  Masaharu Isobe

发明人： Nobuyuki Kurosawa ,  Masaharu Isobe

IPC分类号： C07K16/26

CPC分类号： C07K16/26 ,  C07K16/18 ,  C07K2317/10 ,  C07K2317/20 ,  C07K2317/56 ,  C07K2317/565 ,  C12N5/0635

摘要： [Problem] To provide a method that efficiently produces antigen-specific monoclonal antibodies from a wide range of animal species, and to provide a new antigen-specific monoclonal antibody using this technique. [Solution] A nonhuman animal is immunized with a target antigen, lymph fluid or the like is collected from the immunized nonhuman animal, or lymph fluid or the like is collected from a human having antibodies to the target antigen, the collected lymph fluid or the like is combined with (1) a labeled target antigen and (2) a marker that can selectively binds to plasma cells and/or plasmablasts, and cells that have bound to (1) the labeled target antigen and (2) the marker are then selected. The plasma cells and or the plasmablasts that have specifically bound to the target antigen by the method are selected, an gene of an antibody for the target antigen is collected from the selected cells, the base sequence thereof is identified, an antibody or antibody fragment is prepared on the basis of the base sequence of the identified gene, and an antibody or antibody fragment specific to the target antigen is produced.

摘要翻译： 提供一种从广泛的动物物种有效地产生抗原特异性单克隆抗体的方法，并使用该技术提供新的抗原特异性单克隆抗体。 [解决方案]用目标抗原免疫非人动物，从免疫的非人动物收集淋巴液等，或者从具有针对靶抗原的抗体，收集的淋巴液或 结合（1）标记的靶抗原和（2）可以选择性结合浆细胞和/或浆细胞的标记，以及结合（1）标记的靶抗原的细胞和（2）标记物 选择。 选择通过该方法与靶抗原特异性结合的浆细胞和或浆细胞，从所选择的细胞中收集靶抗原的抗体基因，鉴定其碱基序列，抗体或抗体片段为 基于所鉴定的基因的碱基序列制备，并产生对靶抗原特异性的抗体或抗体片段。

公开(公告)号：US20130023009A1

公开(公告)日：2013-01-24

申请号：US13394153

申请日：2010-09-02

申请人： Nobuyuki Kurosawa ,  Masaharu Isobe

发明人： Nobuyuki Kurosawa ,  Masaharu Isobe

IPC分类号： C12N15/66 ,  C12N15/11 ,  C12N15/63 ,  C12P19/34 ,  C12P21/00

CPC分类号： C12N15/10 ,  C12N15/1027 ,  C12N15/66

摘要： Provided is a method for selectively obtaining, for a given target gene, a “joined DNA fragment” wherein just a target gene fragment is joined with desired other DNA fragments, regardless of whether a DNA fragment containing a target gene sequence has been purified. In the provided method, a double-stranded joining DNA fragment containing a sequence A and/or a sequence B is selectively joined to the ends of a target gene fragment. A mixture of a double-stranded gene fragment, the 3′ end of which is protruding, and the double-stranded joining DNA fragment, which are related in a prescribed manner, undergoes at least two cycles of thermal denaturation, reassociation, and DNA synthesis, resulting in a “joined DNA fragment,” which is a double-stranded DNA fragment including at least one instance of a sequence resulting from joining sequence A, the target gene sequence, and sequence B. A “single-side joined DNA fragment” can also be obtained, by a similar method.

摘要翻译： 提供了对于给定的靶基因，选择性地获得其中仅目标基因片段与所需的其它DNA片段连接的连接的DNA片段，而不管是否已经纯化了含有靶基因序列的DNA片段。 在所提供的方法中，含有序列A和/或序列B的双链连接DNA片段选择性地连接到靶基因片段的末端。 其3'末端突出的双链基因片段和以规定方式相关的双链连接DNA片段的混合物经历至少两个热变性，重新连接和DNA合成的循环 ，得到连接的DNA片段，其是包含由连接序列A，靶基因序列和序列B得到的序列的至少一个实例的双链DNA片段。也可以获得单侧连接的DNA片段 ，通过类似的方法。

公开(公告)号：US4433209A

公开(公告)日：1984-02-21

申请号：US257392

申请日：1981-04-24

申请人： Nobuyuki Kurosawa ,  Kazuaki Suda

发明人： Nobuyuki Kurosawa ,  Kazuaki Suda

IPC分类号： H01R13/70 ,  H01R13/71 ,  H04R5/04 ,  H04S1/00 ,  H04S7/00

CPC分类号： H04R5/04 ,  H04R2420/05

摘要： The present invention is directed to a stereo/monaural selecting circuit having a jack for supplying therefrom for reproduction a stereophonic sound signal output or a monaural sound signal output to apparatus connected thereto, or for receiving for recording a stereophonic sound signal input or a monaural sound signal input from apparatus connected thereto. The jack can be used with either a binaural plug or a monaural plug inserted thereinto, which are attached to stereophonic apparatus or monaural apparatus, respectively, and the circuit selects automatically either a stereophonic operating state or a monaural operating state in response to the type of plug inserted into the jack. In the case where the circuit contains a speaker, the circuit supplies the monaural sound signal output to the speaker when no plug is inserted into the jack.

摘要翻译： 本发明涉及一种立体声/单声道选择电路，其具有用于从其提供的立体声声音信号输出或输出到连接到其上的装置的单声道声音信号的插孔，或者用于接收立体声声音信号输入或单声道声音 从与其连接的装置的信号输入。 千斤顶可以分别与立体声装置或单声道装置连接的双耳插塞或单声道插头一起使用，并且该电路响应于立体声操作状态或单声道操作状态而自动选择立体声操作状态或单声道操作状态 插头插入插孔。 在电路包含扬声器的情况下，当没有插头插入插孔时，电路将单声道声音信号输出到扬声器。

公开(公告)号：US09625464B2

公开(公告)日：2017-04-18

申请号：US13637025

申请日：2011-03-22

申请人： Nobuyuki Kurosawa ,  Masaharu Isobe

发明人： Nobuyuki Kurosawa ,  Masaharu Isobe

IPC分类号： G01N33/58 ,  G01N33/569

CPC分类号： G01N33/582 ,  G01N33/56966

摘要： A method which can isolate plasma cells and plasmablasts efficiently and with high purity, from mammals and birds, without using a cell surface marker is provided.Further disclosed is a fluorescent probe wherein the staining selectivity for the endoplasmic reticulum of cells is higher than the staining selectivity for cell organelles other than the endoplasmic reticulum. Also disclosed is a method for identifying plasma cells and plasmablasts which includes staining cells derived from lymph node tissue or similar by using this probe, and identifying plasma cells and plasmablasts on the basis of the fluorescence intensity from the stained cells. Also disclosed is a fluorescent probe wherein the staining selectivity for cell nuclei is higher than the staining selectivity for cell organelles other than the cell nuclei. Also disclosed is a method for identifying plasma cells and plasmablasts in lymph node tissue or similar which includes staining cells derived from lymph node tissue or similar by using this probe, and identifying plasma cells and plasmablasts on the basis of the fluorescence intensity from the stained cells.

公开(公告)号：US08993241B2

公开(公告)日：2015-03-31

申请号：US12863185

申请日：2009-01-16

申请人： Masaharu Isobe ,  Nobuyuki Kurosawa

发明人： Masaharu Isobe ,  Nobuyuki Kurosawa

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12M3/00 ,  B08B3/04 ,  C07H21/02 ,  C07H21/04 ,  G01N35/00

CPC分类号： B08B3/04 ,  G01N35/0098

摘要： Provided is a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate. The projecting barrier has a cutoff portion and an inner space capable of holding a droplet and having a contact angle to pure water of from 90 to 150 degrees. A reaction method using the reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet by means of a magnet located on the opposite surface of the substrate to thereby conduct reactions and washings.

摘要翻译： 提供了一种可以并行进行多种反应操作和洗涤操作的装置和方法，而不需要在每次操作中洗涤或更换用于转移溶液的仪器。 一种反应装置，其具有设置在基板的一个表面上的一行中的多个突出的屏障。 突出的屏障具有截留部分和能够保持液滴并且具有与90至150度的纯水接触角的内部空间。 使用反应装置的反应方法，其中将固定在磁珠上的物质依次转移到含有表面张力减小剂的溶液的液滴之间，并且通过位于相对表面上的磁体保持在用于保持液滴的空间中 从而进行反应和洗涤。

公开(公告)号：US20120161833A1

公开(公告)日：2012-06-28

申请号：US13316474

申请日：2011-12-10

申请人： Satoshi Yoshida ,  Nobuyuki Kurosawa ,  Kenta Sasaki

发明人： Satoshi Yoshida ,  Nobuyuki Kurosawa ,  Kenta Sasaki

IPC分类号： H03L7/08

CPC分类号： G06F1/3243 ,  G06F1/04 ,  G06F15/78 ,  H03L7/08 ,  H03L7/14 ,  H03L2207/08

摘要： Even after power-down, distinction between a transition from a PLL normal-oscillation state and a transition from a PLL self-oscillation is allowed.A semiconductor device includes a first region which, after having transited from a power-supply state to a power-down state, returns to the power-supply state again, a second region which holds a power source voltage regardless of power-down of the first region, and an oscillator which generates a first clock signal supplied to the first region. The first region includes a PLL circuit. The second region includes an information holding unit capable of holding information which can distinguish whether the operation mode of the PLL circuit is a PLL normal-oscillation mode or a PLL self-oscillation mode, and determines the operation mode of the PLL circuit when the first region has returned from the power-down state to the power-supply state, according to the information held in the information holding unit.

摘要翻译： 即使在掉电之后，允许从PLL正常振荡状态的转变与来自PLL自振荡的转变之间的区别。 半导体器件包括：在从电源状态转换到掉电状态之后再次返回到电源状态的第一区域，第一区域，其保持电源电压，而与第 第一区域和产生提供给第一区域的第一时钟信号的振荡器。 第一区域包括PLL电路。 第二区域包括信息保持单元，其能够保存可以区分PLL电路的操作模式是PLL正常振荡模式还是PLL自振模式的信息，并且当第一个 根据保存在信息保持单元中的信息，区域从断电状态返回到电源状态。

公开(公告)号：US20110020879A1

公开(公告)日：2011-01-27

申请号：US12863185

申请日：2009-01-16

申请人： Masaharu Isobe ,  Nobuyuki Kurosawa

发明人： Masaharu Isobe ,  Nobuyuki Kurosawa

IPC分类号： C12P19/34 ,  C12M1/34

CPC分类号： B08B3/04 ,  G01N35/0098

摘要： The present invention provides a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution and the like in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate, wherein the projecting barrier has at least one cutoff portion and an inner space capable of holding a droplet, and at least the face holding the droplets of the substrate surface has a contact angle to pure water of from 90 to 150 degrees. A reaction method using the above reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet defined by the above-described projecting barriers by means of a magnet located on the opposite surface of the substrate to the surface having the projecting barriers to thereby conduct reactions and washings.

摘要翻译： 本发明提供一种装置和方法，其可以并行地进行多种反应操作和洗涤操作，而不需要在每次操作中洗涤或更换用于转移溶液等的仪器。 一种反应装置，具有设置在基板的一个表面上的一行中的多个突出的屏障，其中所述突出的屏障具有至少一个截止部分和能够保持液滴的内部空间，并且至少保持所述液滴的所述面 衬底表面与纯水的接触角为90至150度。 使用上述反应装置的反应方法，其中将固定在磁珠上的物质依次转移到含有表面张力减小剂的溶液的液滴之间，并且在其表面张力降低剂的液滴之间，所述溶液保持在用于保持由上述突出屏障形成的液滴的空间中 位于基板相对表面上的具有突出阻挡层的磁体的装置，从而进行反应和冲洗。

公开(公告)号：US06218161B1

公开(公告)日：2001-04-17

申请号：US09143438

申请日：1998-08-28

申请人： Shuichi Tsuji ,  Nobuyuki Kurosawa ,  Toshiro Hamamoto ,  Young-Choon Lee ,  Takashi Nakaoka ,  Naoya Kojima

发明人： Shuichi Tsuji ,  Nobuyuki Kurosawa ,  Toshiro Hamamoto ,  Young-Choon Lee ,  Takashi Nakaoka ,  Naoya Kojima

IPC分类号： C12N1554

CPC分类号： C12N9/1081

摘要： Novel GalNAc&agr;2,6-sialyltransferases P-B1 and P-B3; GalNAc&agr;2,6-sialyltransferase genes encoding the above GalNAc&agr;2,6-sialyltransferases P-B1 and P-B3; and an extracellularly releasable protein catalyzing GalNAc&agr;2,6-sialic acid transfer which comprises a polypeptide portion as being an active domain of the GalNAc&agr;2,6-sialyltransferase P-B1 or P-B3 together with a signal peptide are provided. Also provided is a process for preparing a sialyltransferases which enables efficient recovery of a sialyltransferase expressed in a large quantity in microorganisms.

摘要翻译： 新型GalNAcalpha2,6-sialyltransferases P-B1和P-B3; 编码上述GalNAcalpha2,6-唾液酸转移酶P-B1和P-B3的GalNAcalpha2,6-sialyltransferase基因; 以及催化GalNAcalpha2,6-唾液酸转移的细胞外可释放蛋白，其包含作为GalNAcalpha2,6-唾液酸转移酶P-B1或P-B3的活性结构域的多肽部分与信号肽。 还提供了一种制备唾液酸转移酶的方法，其能够有效地回收在微生物中大量表达的唾液酸转移酶。