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    Method for concentrating and purifying hirudin from leeches
    1.
    发明授权
    Method for concentrating and purifying hirudin from leeches 失效
    从水蛭中浓缩和净化水蛭素的方法

    公开(公告)号：US5304310A

    公开(公告)日：1994-04-19

    申请号：US910320

    申请日：1992-07-19

    申请人： Kurt Lang ,  Andreas Kreimeyer

    发明人： Kurt Lang ,  Andreas Kreimeyer

    IPC分类号： C12N15/09 ,  C07D475/04 ,  C07K1/30 ,  C07K14/815 ,  C12N9/00 ,  C12N9/76 ,  C12N15/15 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  B01D11/02 ,  A61K35/62

    CPC分类号： C12N9/6427 ,  C07D475/04 ,  C07K1/30 ,  C07K14/815 ,  C12N9/00 ,  Y10S530/855

    摘要： A method for the concentration and purification of biomolecules is described and comprises introducing the biomolecule into an aqueous/organic solvent system and bringing about a phase separation by addition of salts, where the pH at which the concentration takes place, the solvents suitable for the aqueous/organic solvent system, and the nature of the salt are determined in a series of tests one embodiment concerns concentration of hirudin by using propanol, butanol or acetone a the solvent and sodium chloride as the salt.

    摘要翻译： PCT No.PCT / EP91 / 02269 Sec。 371日期：1992年7月17日 102（e）日期1992年7月17日PCT 1990年12月20日PCT PCT。 出版物WO91 / 10677 日期：1991年7月25日。描述了生物分子的浓缩和纯化方法，包括将生物分子引入水/有机溶剂体系中，并通过加入盐进行相分离，其中发生浓度的pH 适用于水/有机溶剂系统的溶剂和盐的性质在一系列试验中确定，一个实施方案涉及通过使用丙醇，丁醇或丙酮，溶剂和氯化钠作为盐的水蛭素的浓度。

    METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL)
    2.
    发明申请
    METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 审中-公开
    产生胰岛素样生长因子-1和聚（乙二醇）的方法

    公开(公告)号：US20100035817A1

    公开(公告)日：2010-02-11

    申请号：US12576266

    申请日：2009-10-09

    申请人： Stephan Fischer ,  Friederike Hesse ,  Hendrik Knoetgen ,  Kurt Lang ,  Friedrich Metzger ,  Joerg Thomas Regula ,  Christian Schantz ,  Andreas Schaubmar ,  Hans-Joachim Schoenfeld

    发明人： Stephan Fischer ,  Friederike Hesse ,  Hendrik Knoetgen ,  Kurt Lang ,  Friedrich Metzger ,  Joerg Thomas Regula ,  Christian Schantz ,  Andreas Schaubmar ,  Hans-Joachim Schoenfeld

    IPC分类号： A61K38/18 ,  A61P25/28

    CPC分类号： C07K14/65 ,  A61K47/60 ,  C07K2319/21 ,  C07K2319/50

    摘要： The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.

    摘要翻译： 本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。 本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。 该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白，以及 回收赖氨酸 - PEG化IGF-I或IGF-I变体。 本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。 此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。

    Active hedgehog protein conjugate
    4.
    发明授权
    Active hedgehog protein conjugate 失效
    活性刺猬蛋白结合物

    公开(公告)号：US06468978B1

    公开(公告)日：2002-10-22

    申请号：US09301199

    申请日：1999-04-28

    申请人： Angelika Esswein ,  Kurt Lang ,  Petra Rueger ,  Tilman Seytter

    发明人： Angelika Esswein ,  Kurt Lang ,  Petra Rueger ,  Tilman Seytter

    IPC分类号： A61K3817

    CPC分类号： A61K31/185 ,  A61K31/727 ,  A61K38/00 ,  C07K14/47

    摘要： A hedgehog conjugate which is characterized in that it contains: a) a polypeptide composed of 10 to 30 hydrophobic amino acids and/or amino acids which form transmembrane helices and are positively charged, b) 1 to 4 aliphatic, saturated or unsaturated hydrocarbon residues with a chain length of 10 to 24 C atoms and with a hydrophobic action or c) a hydrophobic thio compound covalently bound to a hedgehog protein and which has a several-fold increased activity and is suitable as a pharmaceutical agent.

    摘要翻译： 一种刺猬蛋白缀合物，其特征在于其包含：a）由10至30个疏水氨基酸和/或氨基酸组成的多肽，其形成跨膜螺旋并带正电荷，b）1至4个脂族，饱和或不饱和的烃残基， 链长为10至24个C原子并具有疏水作用，或c）与猬蛋白共价结合的疏水性硫代化合物，并且其具有几倍增加的活性并且适于作为药剂。

    Open end spinning apparatus
    5.
    发明授权
    Open end spinning apparatus 失效
    开口纺纱设备

    公开(公告)号：US5749216A

    公开(公告)日：1998-05-12

    申请号：US818307

    申请日：1997-03-14

    申请人： Kurt Lang ,  Friedbert Schmid

    发明人： Kurt Lang ,  Friedbert Schmid

    IPC分类号： D01H4/08 ,  D01H4/00

    CPC分类号： D01H4/08

    摘要： In the case of an open-end spinning arrangement, a spinning rotor is arranged in a rotor housing, to which a vacuum of at least 500 mm water column is applied. A fibre feed channel, beginning at an opening roller with an entry opening, is directed with an outlet opening at a sliding wall of the spinning rotor. The fibre feed channel bends slightly in the direction towards a fibre collecting groove of the spinning rotor. The cross sectional area of the outlet opening of the fibre feed channel measures at least 19 mm.sup.2 and amounts to at least 30% of the cross sectional area of the entry opening. As a result of the bend in the fibre feed channel, the fibres are collected together in a fibre bundle on a longitudinal side of the fibre feed channel. The fibre bundle occupies only a part area of the outlet opening when leaving the fibre feed channel and is fed with high precision onto the sliding wall. The outlet opening can thus have larger dimensions without this leading to inaccurate feeding of the fibres. The outlet opening possesses dimensions that permit a large volume of air to flow at high speed from the opening roller through the fibre feed channel. Fly accumulation in the area of the opening roller is thus avoided.

    摘要翻译： 在开放式纺纱装置的情况下，在转子壳体内设置有旋转转子，在该壳体内施加至少500mm的水柱的真空。 从具有进入开口的打开辊开始的纤维进料通道与纺丝转子的滑动壁处的出口开口导向。 纤维进料通道沿着纺丝转子的纤维收集槽的方向稍微弯曲。 纤维进料通道的出口开口的横截面积至少为19mm 2，并且相当于入口的横截面面积的至少30％。 由于纤维供给通道的弯曲，纤维在纤维供给通道的纵向侧以纤维束收集在一起。 当离开纤维进料通道时，纤维束仅占出口开口的一部分区域，并且高精度地供给到滑动壁上。 因此，出口开口可以具有更大的尺寸而不导致纤维的不准确的供给。 出口开口具有允许大量空气以高速从打开辊通过纤维进料通道流动的尺寸。 因此避免了在打开辊的区域中的飞累积。

    Feeding and opening device for open-end spinning units with a separation opening for impurities
    6.
    发明授权
    Feeding and opening device for open-end spinning units with a separation opening for impurities 失效
    用于开放式纺纱单元的进料和开启装置，具有用于杂质的分离开口

    公开(公告)号：US4459801A

    公开(公告)日：1984-07-17

    申请号：US392596

    申请日：1982-06-28

    申请人： Fritz Stahlecker ,  Kurt Lang

    发明人： Fritz Stahlecker ,  Kurt Lang

    IPC分类号： D01H4/36 ,  D01H1/12

    CPC分类号： D01H4/36

    摘要： A feeding and opening device for open-end spinning units comprises a feeding roller and a feed table resiliently urged against the feeding roller for feeding a sliver to an opening roller, as well as at least one mechanical supporting element that is located at a spacing from a guide edge of the feed table, while leaving a free space that is open towards the atmosphere between the guide edge and the at least one mechanical supporting element. In accordance with preferred embodiments, the supporting element may be formed of at least one strip located essentially in parallel to an axis of rotation of the opening roller, or at least one row of needles arranged with their free ends directed towards the opening roller.

    摘要翻译： 用于开放式纺纱单元的进料和打开装置包括进料辊和进给辊，所述进料辊和进料辊被弹性地压靠在进料辊上，用于将纱条送入打开辊，以及至少一个机械支撑元件， 馈送台的引导边缘，同时留下在引导边缘和至少一个机械支撑元件之间朝向大气敞开的自由空间。 根据优选实施例，支撑元件可以由基本上平行于开启辊的旋转轴线的至少一个条形成，或者至少一排针布置成其自由端朝向打开辊。

    METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL)
    8.
    发明申请
    METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 有权
    产生胰岛素样生长因子-1和聚（乙二醇）的方法

    公开(公告)号：US20080119409A1

    公开(公告)日：2008-05-22

    申请号：US11846857

    申请日：2007-08-29

    申请人： Stephan Fischer ,  Friederike Hesse ,  Hendrik Knoetgen ,  Kurt Lang ,  Friedrich Metzger ,  Joerg Thomas Regula ,  Christian Schantz ,  Andreas Schaubmar ,  Hans-Joachim Schoenfeld

    发明人： Stephan Fischer ,  Friederike Hesse ,  Hendrik Knoetgen ,  Kurt Lang ,  Friedrich Metzger ,  Joerg Thomas Regula ,  Christian Schantz ,  Andreas Schaubmar ,  Hans-Joachim Schoenfeld

    IPC分类号： A61K38/30 ,  C12P21/06

    CPC分类号： C07K14/65 ,  A61K47/60 ,  C07K2319/21 ,  C07K2319/50

    摘要： The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.

    摘要翻译： 本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。 本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。 该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白，以及 回收赖氨酸 - PEG化IGF-I或IGF-I变体。 本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。 此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。

    Active hedgehog protein conjugate
    9.
    发明申请
    Active hedgehog protein conjugate 审中-公开
    活性刺猬蛋白结合物

    公开(公告)号：US20070191274A1

    公开(公告)日：2007-08-16

    申请号：US11645014

    申请日：2006-12-22

    申请人： Angelika Esswein ,  Kurt Lang ,  Petra Rueger ,  Tilman Seytter

    发明人： Angelika Esswein ,  Kurt Lang ,  Petra Rueger ,  Tilman Seytter

    IPC分类号： A61K38/17 ,  C07K14/47 ,  A61K31/727 ,  A61K31/185

    CPC分类号： A61K31/185 ,  A61K31/727 ,  A61K38/00 ,  C07K14/47

    摘要： A hedgehog conjugate which is characterized in that it contains: a) a polypeptide composed of 10 to 30 hydrophobic amino acids and/or amino acids which form transmembrane helices and are positively charged, b) 1 to 4 aliphatic, saturated or unsaturated hydrocarbon residues with a chain length of 10 to 24 C atoms and with a hydrophobic action or c) a hydrophobic thio compound covalently bound to a hedgehog protein and which has a several-fold increased activity and is suitable as a pharmaceutical agent.

    摘要翻译： 一种刺猬蛋白缀合物，其特征在于其包含：a）由10至30个疏水氨基酸和/或氨基酸组成的多肽，其形成跨膜螺旋并带正电荷，b）1至4个脂族，饱和或不饱和的烃残基， 链长为10至24个C原子并具有疏水作用，或c）与猬蛋白共价结合的疏水性硫代化合物，并且其具有几倍增加的活性并且适于作为药剂。