公开(公告)号：US20060034853A1

公开(公告)日：2006-02-16

申请号：US11129741

申请日：2005-05-16

申请人： Kwok Yuen ,  Chiu Yat Woo ,  Kar Pui Lau ,  Kwok Chan

发明人： Kwok Yuen ,  Chiu Yat Woo ,  Kar Pui Lau ,  Kwok Chan

IPC分类号： A61K39/42 ,  C07H21/04 ,  C12P21/06 ,  C07K14/005

CPC分类号： C12Q1/701 ,  A61K39/12 ,  A61K39/215 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20022 ,  C12N2770/20034

摘要： The present invention provides the complete genomic sequence of a novel human coronavirus, coined as human coronavirus-HKU1 (“HCoV-HKU1”), isolated in Hong Kong. The virus belongs to the order Nidovirales of the family Coronaviridae, being a single-stranded RNA virus of positive polarity. Futher study on nasopharyngeal aspirates from patients with community-acquired pnbeumonia has revealed that there are two genotypes, genotype A and genotype B, for this virus. In addition to the genomic sequences of these two genotypes, the invention provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the HCoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by HCoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the HCoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the HCoV-HKU1.

摘要翻译： 本发明提供了在香港分离的作为人冠状病毒HKU1（“HCoV-HKU1”）的新型人冠状病毒的完整基因组序列。 该病毒属于冠状病毒科家族的Nidovirales，是正极性的单链RNA病毒。 对社区获得性pnbeumonia患者的鼻咽抽吸物的进一步研究表明，该病毒有两种基因型，A型和B型基因型。 除了这两种基因型的基因组序列外，本发明提供了CoV-HKU1完整基因组的推导的氨基酸序列。 HCoV-HKU1的核苷酸序列和推定的氨基酸序列可用于预防，诊断和/或治疗HCoV-HKU1的感染。 此外，本发明基于HCoV-HKU1的核苷酸序列和推定的氨基酸序列，提供使用重组和嵌合形式以及HCoV-HKU1的亚基的免疫原性和疫苗制剂。

公开(公告)号：US20050181357A1

公开(公告)日：2005-08-18

申请号：US10807807

申请日：2004-03-24

申请人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls ,  Frederick Leung

发明人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls ,  Frederick Leung

IPC分类号： A61P11/00 ,  A61P31/14 ,  C12N15/50 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/02 ,  C07K14/165 ,  C07K16/10

CPC分类号： C12Q1/701 ,  C12Q1/6844 ,  Y02A50/451

摘要： The present invention relates to a high-throughput diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a high-throughput reverse transcription-PCR diagnostic test for SARS associated coronavirus (SARS-CoV). The present assay is a rapid, reliable assay which can be used for diagnosis and monitoring the spread of SARS and is based on the nucleotide sequences of the N (nucleocapsid)-gene of the hSARS virus. The present method eliminates false negative results and provides increased sensitivity for the assay. The invention also discloses the S (spike)-gene of the hSARS virus. The invention further relates to the deduced amino acid sequences of the N-gene and S-gene products of the hSARS virus and to the use of the N-gene and S-gene products in diagnostic methods. The invention further encompasses diagnostic assays and kits comprising antibodies generated against the N-gene or S-gene product.

摘要翻译： 本发明涉及用于引起人类严重急性呼吸综合征（SARS）的病毒（“hSARS病毒”）的高通量诊断测定法。 特别地，本发明涉及SARS相关冠状病毒（SARS-CoV）的高通量逆转录PCR诊断试验。 本试验是一种快速，可靠的测定法，可用于诊断和监测SARS的扩散，并且基于hSARS病毒的N（核衣壳）基因的核苷酸序列。 本方法消除假阴性结果并提供增加的检测灵敏度。 本发明还公开了hSARS病毒的S（尖峰）基因。 本发明还涉及hSARS病毒的N-基因和S-基因产物的推断氨基酸序列以及在诊断方法中使用N-基因和S-基因产物。 本发明还包括诊断测定和试剂盒，其包含针对N-基因或S-基因产物产生的抗体。

公开(公告)号：US20060018923A1

公开(公告)日：2006-01-26

申请号：US10895064

申请日：2004-07-21

申请人： Kwok Yuen ,  Chiu Woo ,  Kar Lau ,  Kwok Chan ,  Lit Poon ,  Joseph Peiris ,  Yi Guan

发明人： Kwok Yuen ,  Chiu Woo ,  Kar Lau ,  Kwok Chan ,  Lit Poon ,  Joseph Peiris ,  Yi Guan

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N15/86 ,  C07K14/01

CPC分类号： G01N33/56983 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/20021 ,  C12N2770/20022

摘要： The present invention provides the complete genomic sequence of a novel human coronavirus, coined as coronavirus-HKU1 (“CoV-HKU1”), isolated in Hong Kong from a patient who had a recent history of visit to Schenzhen, China. The virus belongs to the order Nidovirales of the family Coronaviridae, being a single-stranded RNA virus of positive polarity. The invention also provides the deduced amino acid sequences of the complete genome of the CoV-HKU1. The nucleotide sequences and deduced amino acid sequences of the CoV-HKU1 are useful in preventing, diagnosing and/or treating the infection by CoV-HKU1. Furthermore, the invention provides immunogenic and vaccine preparations using recombinant and chimeric forms as well as subunits of the CoV-HKU1 based on the nucleotide sequences and deduced amino acid sequences of the CoV-HKU1.

摘要翻译： 本发明提供了一种新型人冠状病毒的完整基因组序列，其创造为冠状病毒HKU1（“CoV-HKU1”），在香港从最近访问中国的深圳的病人中分离出来。 该病毒属于冠状病毒科家族的Nidovirales，是正极性的单链RNA病毒。 本发明还提供了CoV-HKU1的完整基因组的推导的氨基酸序列。 CoV-HKU1的核苷酸序列和推导的氨基酸序列可用于预防，诊断和/或治疗CoV-HKU1感染。 此外，本发明基于CoV-HKU1的核苷酸序列和推定的氨基酸序列，提供使用重组和嵌合形式以及CoV-HKU1的亚基的免疫原性和疫苗制剂。

公开(公告)号：US20050215494A1

公开(公告)日：2005-09-29

申请号：US10983985

申请日：2004-11-08

申请人： Kwok Yuen ,  Feng Chen ,  Kwok Chan ,  Yi Kao ,  Yi Guan ,  Leo Poon ,  Jsm Peiris

发明人： Kwok Yuen ,  Feng Chen ,  Kwok Chan ,  Yi Kao ,  Yi Guan ,  Leo Poon ,  Jsm Peiris

IPC分类号： A61K31/382 ,  A61K31/7048

CPC分类号： A61K31/382 ,  A61K31/7048

摘要： The present invention relates to a pharmaceutical composition useful for the treatment of diseases associated with viruses of the order Nidovirales of the family Coronaviradae, such as Severe Acute Respiratory Syndrome (SARS) viruses in humans and other animals. In particular, this invention relates to a naturally occurring compound, namely, baicalin, extracted and purified from the Chinese medicinal plant Scutellaria baicalensis Georgi (Chinese name: Huang Qin), that exhibits potent antiviral activity against members of the order Nidovirales of the family Coronaviradae that infects humans and other animals; in particular, SARS viruses in humans (“hSARS virus”). The invention also relates to a therapeutic method, using pharmaceutical compositions comprising baicalin compounds, for the treatment, amelioration, management or prevention of diseases associated with members of the order Nidovirales of the family Coronaviradae, such as hSARS.

摘要翻译： 本发明涉及可用于治疗与冠状病毒家族的病毒相关的疾病的药物组合物，例如人和其他动物中的严重急性呼吸综合征（SARS）病毒。 特别地，本发明涉及一种天然存在的化合物，即黄芩苷，从中药材黄芩（中文名：黄秦）中提取和纯化，对抗冠状病毒家族的Nidovirales成员表现出强烈的抗病毒活性 感染人类和其他动物; 特别是人类中的SARS病毒（“hSARS病毒”）。 本发明还涉及使用包含黄芩苷化合物的药物组合物用于治疗，改善，管理或预防与冠状病毒家族（Norovirales）家族成员有关的疾病如hSARS的治疗方法。

公开(公告)号：US20050009009A1

公开(公告)日：2005-01-13

申请号：US10808187

申请日：2004-03-24

申请人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls

发明人： Joseph Peiris ,  Kwok Yuen ,  Lit Poon ,  Yi Guan ,  Kwok Chan ,  John Nicholls

IPC分类号： C07H21/04 ,  C07K16/08 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/701 ,  C07H21/04 ,  C12Q1/6844

摘要： The present invention relates to a diagnostic assay for the virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). In particular, the invention relates to a real-time quantitative PCR assay for the detection of hSARS virus using reverse transcription and polymerase chain reaction. Specifically, the quantitative assay is a TaqMan® assay using the primers and probes constructed based on the genome of the hSARS virus. The invention further relates to a diagnostic kit that comprises nucleic acid molecules for the detection of the hSARS virus.

摘要翻译： 本发明涉及在人类中引起严重急性呼吸综合征（SARS）的病毒的诊断测定法（“hSARS病毒”）。 特别地，本发明涉及使用逆转录和聚合酶链反应检测hSARS病毒的实时定量PCR测定。 具体地，定量测定是使用基于hSARS病毒基因组构建的引物和探针的TaqMan测定法。 本发明还涉及包含用于检测hSARS病毒的核酸分子的诊断试剂盒。