公开(公告)号：US6162931A

公开(公告)日：2000-12-19

申请号：US631202

申请日：1996-04-12

申请人： Kyle R. Gee ,  Martin Poot ,  Dieter H. Klaubert ,  Wei-Chuan Sun ,  Richard P. Haugland ,  Fei Mao

发明人： Kyle R. Gee ,  Martin Poot ,  Dieter H. Klaubert ,  Wei-Chuan Sun ,  Richard P. Haugland ,  Fei Mao

IPC分类号： G01N33/48 ,  C07B61/00 ,  C07C37/00 ,  C07C37/055 ,  C07C39/27 ,  C07C205/26 ,  C07C205/37 ,  C07C209/36 ,  C07C213/00 ,  C07C217/82 ,  C07C245/20 ,  C07D311/82 ,  C07D493/10 ,  C07F9/6561 ,  C07K1/13 ,  C07K16/18 ,  C09B11/00 ,  C09B11/24 ,  C09B11/28 ,  G01N1/30 ,  G01N21/76 ,  C07D311/78 ,  C07D311/88 ,  C07D471/00

CPC分类号： C09B11/00 ,  C07C37/055 ,  C07C41/18 ,  C07C201/08 ,  C07C201/12 ,  C07C205/26 ,  C07C205/37 ,  C07C209/10 ,  C07C213/02 ,  C07C213/08 ,  C07C245/20 ,  C07D311/82 ,  C09B11/24 ,  G01N1/30 ,  Y10S430/116 ,  Y10S430/126 ,  C07C39/245 ,  C07C211/52 ,  C07C217/84 ,  C07C215/76 ,  C07C43/225

摘要： The family of dyes of the invention are fluoresceins and rhodols that are directly substituted on one or more aromatic carbons by fluorine. These fluorine-substituted fluorescent dyes possess greater photostability and have lower sensitivity to pH changes in the physiological range of 6-8 than do non-fluorinated dyes, exhibit less quenching when conjugated to a substance, and possess additional advantages. The dyes of the invention are useful as detectable tracers and for preparing conjugates of organic and inorganic substances.

摘要翻译： 本发明的染料族是在一个或多个芳族碳上被氟直接取代的荧光素和二醇。 这些氟取代的荧光染料具有更高的光稳定性，并且对于6-8生理范围内的pH变化的敏感性低于非氟化染料，当与物质缀合时表现出较少的淬灭，并且具有额外的优点。 本发明的染料可用作可检测的示踪剂并用于制备有机和无机物质的共轭物。

公开(公告)号：US06291203B1

公开(公告)日：2001-09-18

申请号：US09266697

申请日：1998-12-03

申请人： Martin Poot ,  Fei Mao

发明人： Martin Poot ,  Fei Mao

IPC分类号： G01N130

CPC分类号： G01N1/30 ,  C09B23/02 ,  G01N2001/302

摘要： The present invention relates to the use of positively-charged unsymmetrical cyanine dyes, including novel unsymmetrical cyanine dyes, to stain cells and selectively stain intracellular organelles, with particular advantages in staining mitochondria. The dyes are generally weakly fluorescent in aqueous solution but highly fluorescent in cells and organelles, typically staining cells or mitochondria with a green fluorescence. The dyes stain mitochondria in both live and dead cells, and are retained in mitochondria even where the cells are treated with solvents that permeabilize cell and mitochondrial membranes. Mitochondria or cells stained according to this method are optionally stained with an additional detection reagent, such as a labeled antibody, labeled oligonucleotide, fluorogenic enzyme substrate, or other indicator for a specific cellular component or substructure, including another unsymmetrical cyanine stain of the same or different class.

摘要翻译： 本发明涉及使用带正电荷的不对称花青染料，包括新颖的不对称花青染料，染色细胞和选择性染色细胞内细胞器，具有染色线粒体特别的优点。 染料通常在水溶液中弱荧光，但在细胞和细胞器中高度荧光，通常用绿色荧光染色细胞或线粒体。 染料在活细胞和死细胞中染色线粒体，并且保留在线粒体中，甚至在用细胞和线粒体膜透化的溶剂处理细胞的情况下。 线粒体或根据该方法染色的细胞任选地用另外的检测试剂如标记的抗体，标记的寡核苷酸，荧光酶底物或用于特定细胞组分或亚结构的其它指示剂进行染色，包括其它不对称花青染色 不同班