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公开(公告)号：US07087422B2
公开(公告)日：2006-08-08
申请号：US10664958
申请日：2003-09-22
申请人： Kyoko Koibuchi , Daiki Ninomiya , Mari Kojima , Yoichi Ueda , Jun-ichi Maruyama , Katsuhiko Kitamoto
发明人： Kyoko Koibuchi , Daiki Ninomiya , Mari Kojima , Yoichi Ueda , Jun-ichi Maruyama , Katsuhiko Kitamoto
CPC分类号： C12N9/62
摘要： The object of the present invention is to provide Koji mold aminopeptidases capable of efficiently hydrolyzing persistent peptides and also genes encoding the aminopeptidases. The present invention provides Aspergillus nidulans aminopeptidase and nucleic acid molecules encoding it. In particular, the present invention provides a protein having an amino acid sequence represented by amino acid Nos. 1 to 519 in SEQ ID NO: 2, or a protein containing the substitution, deletion, insertion, addition or inversion of one or more amino acids in said sequence, and which protein has an activity of catalyzing the reaction for releasing an amino acid at an N-terminal of a peptide, and nucleic acid molecules encoding them.
摘要翻译： 本发明的目的是提供能够有效水解持久性肽的Koji模具氨基肽酶以及编码氨基肽酶的基因。 本发明提供构巢曲霉氨基肽酶和编码它的核酸分子。 特别地，本发明提供了具有SEQ ID NO：2中的氨基酸编号1〜519所示的氨基酸序列的蛋白质，或含有一个或多个氨基酸的取代，缺失，插入，添加或倒置的蛋白质 并且哪个蛋白质具有催化用于释放肽的N末端的氨基酸的反应的活性和编码它们的核酸分子的活性。
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公开(公告)号：US06830905B2
公开(公告)日：2004-12-14
申请号：US10262083
申请日：2002-10-02
IPC分类号： C12P1314
摘要： Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library hybridization using the partial sequence as a probe.
摘要翻译： 从米曲霉纯化谷氨酰胺酶，测定其部分氨基酸序列，通过基于所得信息的PCR获得谷氨酰胺酶基因的部分序列，以及含有来自米曲霉基因组文库和cDNA文库的构建谷氨酰胺酶基因的DNA片段，构巢曲霉（Aspergillus nodulans） 使用部分序列作为探针的基因组文库杂交。
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公开(公告)号：US20070020726A1
公开(公告)日：2007-01-25
申请号：US11522323
申请日：2006-09-18
摘要： Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library by hybridization using the partial sequence as a probe.
摘要翻译： 从米曲霉纯化谷氨酰胺酶，测定其部分氨基酸序列，通过基于所得信息的PCR获得谷氨酰胺酶基因的部分序列，以及含有来自米曲霉基因组文库和cDNA文库的构建谷氨酰胺酶基因的DNA片段，构巢曲霉（Aspergillus nodulans） 使用部分序列作为探针进行杂交的基因组文库。
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公开(公告)号：US07157252B2
公开(公告)日：2007-01-02
申请号：US10851337
申请日：2004-05-24
摘要： Glutaminase is purified from an Aspergillus oryzae, its partial amino acid sequence is determined, a partial sequence of glutaminase gene is obtained by PCR based on the obtained information, and DNA fragments containing glutaminase gene from Aspergillus oryzae genomic library and cDNA library, and Aspergillus nidulans genomic library by hybridization using the partial sequence as a probe.
摘要翻译： 从米曲霉纯化谷氨酰胺酶，测定其部分氨基酸序列，通过基于所得信息的PCR获得谷氨酰胺酶基因的部分序列，以及含有来自米曲霉基因组文库和cDNA文库的谷氨酰胺酶基因的DNA片段，构巢曲霉（Aspergillus nodulans） 使用部分序列作为探针进行杂交的基因组文库。
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公开(公告)号：US20050120394A1
公开(公告)日：2005-06-02
申请号：US10490046
申请日：2002-09-13
申请人： Satoshi Saitoh , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Katsuhiko Kitamoto
发明人： Satoshi Saitoh , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Katsuhiko Kitamoto
IPC分类号： C12N1/19 , C12N1/21 , C12N15/69 , C12N15/81 , A01K67/00 , A01H1/00 , C07H21/04 , C12N1/18 , C12N9/06 , C12N15/74
摘要： The present invention relates to a method of expressing a gene by inserting the genome into a host organism using genetic engineering techniques. The present invention further relates to a novel promoter, a recombinant vector containing the promoter and a target gene, a transformant containing the recombinant vector, and a method of producing a useful gene product or useful substance using the transformant.
摘要翻译： 本发明涉及通过使用基因工程技术将基因组插入宿主生物体来表达基因的方法。 本发明还涉及一种新型启动子，含有启动子和靶基因的重组载体，含有重组载体的转化体，以及使用该转化体生产有用的基因产物或有用物质的方法。
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公开(公告)号：US20110020926A1
公开(公告)日：2011-01-27
申请号：US12896676
申请日：2010-10-01
申请人： Keiko ABE , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
发明人： Keiko ABE , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
摘要： Disclosed is a polypeptide shown below in (A) or (B), a protein dimer neoculin comprising the polypeptide Neoculin Acidic Subunit (NAS) and the polypeptide Neoculin Basic Subunit (NBS) and having a taste-modifying activity: (A) a polypeptide comprising an amino acid sequence shown in SEQ ID NO.2 in the sequence listing; (B) a polypeptide comprising an amino acid sequence with the substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence shown in SEQ ID NO. 2 in the sequence listing, which polypeptide can form the neoculin dimer having a taste-modifying activity together with the polypeptide NBS.
摘要翻译： 公开了下述（A）或（B）所示的多肽，其包含多肽新霉素酸性亚基（NAS）和多肽新霉素碱基亚基（NBS）并具有调味活性的蛋白质二聚体新霉素：（A）多肽 包含序列表中SEQ ID NO：2所示的氨基酸序列; （B）包含SEQ ID NO：1所示的氨基酸序列中的一个或几个氨基酸的取代，缺失，插入，添加或倒位的氨基酸序列的多肽。 2，该多肽可以与多肽NBS一起形成具有调味活性的新霉素二聚体。
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7.发明授权公开(公告)号：US08383798B2
公开(公告)日：2013-02-26
申请号：US12529544
申请日：2008-01-18
申请人： Shigeharu Moriya , Toshiaki Kudo , Tetsushi Inoue , Nemuri Todaka , Katsuhiko Kitamoto , Manabu Arioka , Junichi Maruyama
发明人： Shigeharu Moriya , Toshiaki Kudo , Tetsushi Inoue , Nemuri Todaka , Katsuhiko Kitamoto , Manabu Arioka , Junichi Maruyama
IPC分类号： C07H21/04
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091
摘要： This invention provides: DNA encoding cellulase enzymes from intestinal symbiotic protists of the insects of Reticulitermes speratus, Hodotermopsis sjostedti, Neotermes koshunensis, Mastotermes darwiniensis, and Cryptocercidae, comprising the nucleotide sequences as shown in SEQ ID NOS:1 to 140; an expression system for the DNA; and a method for producing the cellulase enzymes using the expression system.
摘要翻译： 本发明提供：编码纤维素酶的纤维素酶的DNA，所述纤维素酶包含由SEQ ID NO：1至140所示的核苷酸序列，所述纤维素酶含有丝状真菌， DNA表达系统; 以及使用表达系统生产纤维素酶的方法。
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公开(公告)号：US08043851B2
公开(公告)日：2011-10-25
申请号：US12896676
申请日：2010-10-01
申请人： Keiko Abe , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
发明人： Keiko Abe , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
摘要： Disclosed is a polypeptide shown in the following in (A), and a protein dimer neoculin comprising the polypeptide Neoculin Acidic Subunit (NAS) and the polypeptide Neoculin Basic Subunit (NBS) and having a taste-modifying activity: (A) a polypeptide comprising an amino acid sequence shown in SEQ ID NO.2 in the sequence listing.
摘要翻译： 公开了以下（A）中所示的多肽和包含多肽新霉素酸性亚基（NAS）和多肽新霉素基础亚基（NBS）并具有调味活性的蛋白质二聚体新霉素：（A）包含 序列表中SEQ ID NO：2所示的氨基酸序列。
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9.发明申请公开(公告)号：US20080305522A1
公开(公告)日：2008-12-11
申请号：US10587539
申请日：2005-01-27
申请人： Keiko Abe , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
发明人： Keiko Abe , Tomiko Asakura , Hiroyuki Sorimachi , Tazuko Uenoyama , Kenichiro Nakajima , Katsuhiko Kitamoto , Junichi Maruyama , Mikiya Kishi
摘要： It is an object of the invention to find a substance with a better taste-modifying function and determine the structure of the taste-modifying substance, as well as to elucidate the structure thereof at a gene level and determine the primary structure of the substance and obtain the gene encoding the substance. Additionally, it is an object of the invention to provide a novel taste-modifying composition characteristically containing the taste-modifying substance.Specifically, the invention provides the polypeptide shown below in (A) or (B), a protein dimer neoculin comprising the polypeptide NAS and the polypeptide NBS and having a taste-modifying activity:(A) a polypeptide comprising an amino acid sequence shown in SEQ ID NO.2 in the sequence listing; (B) a polypeptide comprising an amino acid sequence with the substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence shown in SEQ ID NO.2 in the sequence listing, which polypeptide can form the neoculin dimer having a taste-modifying activity together with the polypeptide NBS.
摘要翻译： 本发明的目的是找到具有更好的味觉调节功能的物质并确定调味物质的结构，以及阐明其在基因水平上的结构并确定该物质的一级结构和 获得编码该物质的基因。 此外，本发明的目的是提供一种特征性地含有味道调节物质的新颖的味道改良组合物。 具体而言，本发明提供下述（A）或（B）所示的多肽，包含多肽NAS和多肽NBS并具有调味活性的蛋白质二聚体新霉素：（A）包含氨基酸序列 序列表中SEQ ID NO：2; （B）包含在序列表中SEQ ID NO.2所示的氨基酸序列中的一个或几个氨基酸的取代，缺失，插入，添加或倒位的氨基酸序列的多肽，该多肽可以形成新霉素 二聚体与多肽NBS一起具有调味活性。
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公开(公告)号：US20070004003A1
公开(公告)日：2007-01-04
申请号：US10547708
申请日：2004-03-02
申请人： Katsuhiko Kitamoto , Manabu Arioka , Shotaro Yamaguchi , Masayuki Machida , Keletsu Abe , Katsuya Gomi , Kiyoshi Asai , Motoaki Sano , Taishin Kin , Hideki Nagasaki , Akira Hosoyama , Osamu Akita , Naotake Ogasawara , Satoru Kuhara
发明人： Katsuhiko Kitamoto , Manabu Arioka , Shotaro Yamaguchi , Masayuki Machida , Keletsu Abe , Katsuya Gomi , Kiyoshi Asai , Motoaki Sano , Taishin Kin , Hideki Nagasaki , Akira Hosoyama , Osamu Akita , Naotake Ogasawara , Satoru Kuhara
CPC分类号： C12N9/20 , C12Y301/01004
摘要： It is intended to provide koji mold-origin phospholipase A2 and a DNA encoding it. Namely, phospholipase A2 comprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A2.
摘要翻译： 旨在提供曲霉起源磷脂酶A 2 N 2和编码它的DNA。 即，包含以下蛋白质（a）或（b）的磷脂酶A 2 ：（a）具有SEQ ID NO：1或2所示的氨基酸序列的蛋白质; 和（b）通过部分修饰具有源自SEQ ID NO：1或2所示的氨基酸序列的氨基酸序列并用作磷脂酶A 2的蛋白质。
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