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公开(公告)号：US20230265463A1
公开(公告)日：2023-08-24
申请号：US18183669
申请日：2023-03-14
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Charles F. Rice , Ryan Skinner , Trisha Barrett , Aaron Argyros
CPC classification number: C12P7/10 , C12N1/18 , C12N9/2402 , C12N15/81 , C12P7/20 , C12Y302/01028 , C12P19/12
Abstract: The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).
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公开(公告)号：US20230193232A1
公开(公告)日：2023-06-22
申请号：US18049055
申请日：2022-10-24
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Trisha Barrett , Aaron Argyros
CPC classification number: C12N9/2402 , C12N1/18 , C12N9/48 , C12N9/1051 , C12N9/2405 , C12P7/10 , C12Y204/01001 , C12Y302/01028
Abstract: The present disclosure concerns a recombinant yeast host cell exhibiting higher stability and, in some embodiments, higher fermentation performance. The recombinant yeast host cell stability has a limited ability to express an hydrolase during its propagation phase. In return, this limits the cleavage of a yeast cellular component during or after propagation which may be detrimental to the stability and/or fermentation performances. The recombinant yeast host cell expresses a heterologous hydrolase under the control of a heterologous promoter (for limiting the expression of the heterologous hydrolase during propagation and favoring the expression of the heterologous hydrolase during fermentation).
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公开(公告)号：US20220220487A1
公开(公告)日：2022-07-14
申请号：US17292679
申请日：2019-11-13
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Trisha Barrett , Ryan Skinner , Aaron Argyros
Abstract: The present disclosure concerns a recombinant yeast host cell having a first genetic modification for expressing an heterologous trehalase, and a second genetic modification for increasing trehalose production. The present disclosure also concerns a process using the recombinant yeast host cell for making a fermented product, such as ethanol.
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4.发明授权公开(公告)号：US11193130B2
公开(公告)日：2021-12-07
申请号：US16426563
申请日：2019-05-30
Inventor： Elena Brevnova , John E. McBride , Erin Wiswall , Kevin S. Wenger , Nicky Caiazza , Heidi Hau , Aaron Argyros , Frank Agbogbo , Charles F. Rice , Trisha Barrett , John S. Bardsley , Abigail Foster , Anne K. Warner , Mark Mellon , Ryan Skinner , Indraneel Shikhare , Riaan Den Haan , Chhayal V. Gandhi , Alan Belcher , Vineet B. Rajgarhia , Allan C. Froehlich , Kristen M. Deleault , Emily Stonehouse , Shital A. Tripathi , Jennifer Gosselin , Yin-Ying Chiu , Haowen Xu
Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.
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公开(公告)号：US10604772B2
公开(公告)日：2020-03-31
申请号：US14771831
申请日：2014-03-13
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Aaron Argyros , Trisha Barrett
IPC: C12P7/06 , C12N9/06 , C12N9/00 , C12N15/52 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/34 , C12N9/50 , C12P7/10
Abstract: The present invention provides for a mechanism to reduce glycerol production and increase nitrogen utilization and ethanol production of recombinant microorganisms. One aspect of this invention relates to strains of S. cerevisiae with reduced glycerol productivity that get a kinetic benefit from higher nitrogen concentration without sacrificing ethanol yield. A second aspect of the invention relates to metabolic modifications resulting in altered transport and/or intracellular metabolism of nitrogen sources present in com mash.
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Patent Agency Ranking
6.发明授权公开(公告)号：US10294484B2
公开(公告)日：2019-05-21
申请号：US14936840
申请日：2015-11-10
Inventor： Elena Brevnova , John E. McBride , Erin Wiswall , Kevin S. Wenger , Nicky Caiazza , Heidi Lau , Aaron Argyros , Frank Agbogbo , Charles F. Rice , Trisha Barrett , John S. Bardsley , Abigail Foster , Anne K. Warner , Mark Mellon , Ryan Skinner , Indraneel Shikhare , Riaan Den Haan , Chhayal V. Gandhi , Alan Belcher , Vineet B. Rajgarhia , Allan C. Froehlich , Kristen M. Deleault , Emily Stonehouse , Shital A. Tripathi , Jennifer Gosselin , Yin-Ying Chiu , Haowen Xu
Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.
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公开(公告)号：US20240360484A1
公开(公告)日：2024-10-31
申请号：US18443045
申请日：2024-02-15
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Aaron Argyros , Trisha Barrett
IPC: C12P7/06 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/06 , C12N9/10 , C12N9/34 , C12N9/50 , C12N9/60 , C12N15/52 , C12P7/10
CPC classification number: C12P7/06 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/0016 , C12N9/1029 , C12N9/2428 , C12N9/50 , C12N9/60 , C12N9/93 , C12N15/52 , C12Y104/01002 , C12Y104/01004 , C12Y104/01014 , C12Y603/01002 , C12Y603/04006 , C12P7/10 , C12Y101/01001 , C12Y101/01002 , C12Y102/0101 , C12Y197/01004 , C12Y203/01054 , C12Y302/01003 , C12Y304/23041 , Y02E50/10
Abstract: The present invention provides for a mechanism to reduce glycerol production and increase nitrogen utilization and ethanol production of recombinant microorganisms. One aspect of this invention relates to strains of S. cerevisiae with reduced glycerol productivity that get a kinetic benefit from higher nitrogen concentration without sacrificing ethanol yield. A second aspect of the invention relates to—metabolic modifications resulting in altered transport and/or intracellular metabolism of nitrogen sources present in corn mash.
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公开(公告)号：US11655484B2
公开(公告)日：2023-05-23
申请号：US17098057
申请日：2020-11-13
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Arthur J. Shaw, IV , Aaron Argyros , Trisha Barrett
IPC: C12N9/88 , C12P7/06 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/12 , C12N15/52 , C12N1/16 , C12N9/16 , C12N9/90
CPC classification number: C12P7/06 , C12N1/16 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/1029 , C12N9/1217 , C12N9/16 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y101/01001 , C12Y101/01008 , C12Y102/0101 , C12Y197/01004 , C12Y202/01001 , C12Y202/01002 , C12Y203/01008 , C12Y203/01054 , C12Y207/02012 , C12Y301/03021 , C12Y401/01001 , C12Y401/02009 , C12Y401/02022 , C12Y501/03001 , C12Y503/01006 , Y02E50/10
Abstract: The present invention provides for a mechanism to completely replace the electron accepting function of glycerol formation with an alternative pathway to ethanol formation, thereby reducing glycerol production and increasing ethanol production. In some embodiments, the invention provides for a recombinant microorganism comprising a down-regulation in one or more native enzymes in the glycerol-production pathway. In some embodiments, the invention provides for a recombinant microorganism comprising an up-regulation in one or more enzymes in the ethanol-production pathway.
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公开(公告)号：US20230063426A1
公开(公告)日：2023-03-02
申请号：US17929986
申请日：2022-09-06
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Trisha Barrett , Charles F. Rice , Aaron Argyros
Abstract: The present disclosure relates to proteases for improving alcoholic fermentation. The proteases are expressed from a recombinant host cell. The present disclosure also provides a population of recombinant host cells expressing an heterologous protease that can be used in combination with recombinant host cells expressing an heterologous glucoamylase and/or an heterologous glycerol reduction system.
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公开(公告)号：US20220177930A1
公开(公告)日：2022-06-09
申请号：US17592915
申请日：2022-02-04
Applicant: Lallemand Hungary Liquidity Management LLC
Inventor： Aaron Argyros , Trisha Barrett
IPC: C12P7/06 , C12N9/06 , C12N9/00 , C12N15/52 , C12N9/60 , C12N9/50 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/34
Abstract: The present invention provides for a mechanism to reduce glycerol production and increase nitrogen utilization and ethanol production of recombinant microorganisms. One aspect of this invention relates to strains of S. cerevisiae with reduced glycerol productivity that get a kinetic benefit from higher nitrogen concentration without sacrificing ethanol yield. A second aspect of the invention relates to metabolic modifications resulting in altered transport and/or intracellular metabolism of nitrogen sources present in corn mash.
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