公开(公告)号：US11692210B2

公开(公告)日：2023-07-04

申请号：US17464139

申请日：2021-09-01

申请人： Braskem S.A.

发明人： Iuri Estrada Gouvea ,  Marcos Rogerio Simoes ,  Mariana Trovo Marchesin ,  Aline Silva Romão Dumaresq

IPC分类号： C12N15/81 ,  C12P17/04 ,  C12N9/88 ,  C12N9/10 ,  C12N9/12 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04

CPC分类号： C12P17/04 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/93 ,  C12Y101/01001 ,  C12Y102/0101 ,  C12Y203/01008 ,  C12Y207/01019 ,  C12Y207/02012 ,  C12Y401/01039 ,  C12Y401/02009 ,  C12Y401/02022 ,  C12Y602/01001 ,  C12N15/81

摘要： The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.

公开(公告)号：US09518278B2

公开(公告)日：2016-12-13

申请号：US14211415

申请日：2014-03-14

申请人： The Regents of the University of California

发明人： James C. Liao ,  Igor Bogorad

IPC分类号： C12P9/00 ,  C12N15/63 ,  C12N9/88 ,  C12N15/52

CPC分类号： C12N15/52 ,  C12N9/88 ,  C12N15/63 ,  C12N15/70 ,  C12P9/00 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02P20/52

摘要： Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.

摘要翻译： 提供了催化从甲醇，甲烷和/或甲醛合成化学物质和生物化学物质的微生物。 还提供了产生这种生物体的方法和使用这种生物体合成化学品和生物化学品的方法。

公开(公告)号：US20140273165A1

公开(公告)日：2014-09-18

申请号：US14211415

申请日：2014-03-14

申请人： The Regents of the University of California

发明人： James C. Liao ,  Igor Bogorad

IPC分类号： C12P9/00 ,  C12N15/63

CPC分类号： C12N15/52 ,  C12N9/88 ,  C12N15/63 ,  C12N15/70 ,  C12P9/00 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02P20/52

摘要： Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.

摘要翻译： 提供了催化从甲醇，甲烷和/或甲醛合成化学物质和生物化学物质的微生物。 还提供了产生这种生物体的方法和使用这种生物体合成化学品和生物化学品的方法。

公开(公告)号：US20240306693A1

公开(公告)日：2024-09-19

申请号：US18263830

申请日：2022-02-02

申请人： BONUMOSE, INC.

发明人： Daniel Joseph WICHELECKI ,  Edwin O. ROGERS

IPC分类号： A23L33/20 ,  A21D2/26 ,  A23L33/17 ,  C12N9/12 ,  C12N9/90 ,  C12N9/92

CPC分类号： A23L33/20 ,  A21D2/26 ,  A23L33/17 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12Y207/01144 ,  C12Y401/02022 ,  C12Y503/01009 ,  C12Y504/02002

摘要： The invention relates to preparation of food ingredients enriched with a low-glycemic sugar replacement through enzymatic conversion. Food ingredients may be enriched with, for example, D-tagatose, D-allulose, D-allose, D-mannose, D-talose, and/or inositol by enzymatically converting saccharides found in flour, meal, ground tuber, ground pulse, ground bark, starch, malted grain or malt extract, maltodextrin, cellulose, cellodextrin, any of their derivatives (e.g., amylose, amylopectin, dextrin, cellobiose, etc.), and/or sucrose into D-tagatose, D-allulose, D-allose, D-mannose, D-talose and/or inositol. The enriched material can be used as a food ingredient instead of the low-glycemic sugar being purified for use as a food ingredient.

公开(公告)号：US20180201958A1

公开(公告)日：2018-07-19

申请号：US15744049

申请日：2016-07-21

申请人： The Regents of the University of California

发明人： James U. Bowie ,  Tyler P. Korman ,  Paul H. Opgenorth

IPC分类号： C12P7/16 ,  C12P7/62

CPC分类号： C12P7/16 ,  C12P7/625 ,  C12Y101/01044 ,  C12Y202/01001 ,  C12Y202/01002 ,  C12Y203/01008 ,  C12Y301/01031 ,  C12Y401/02009 ,  C12Y401/02013 ,  C12Y401/02022 ,  C12Y501/03001 ,  C12Y503/01001 ,  C12Y503/01006 ,  C12Y503/01009 ,  Y02E50/10

摘要： Provided is an engineered pathway that can function in a cell-free system, cellular system or a combination thereof to convert a sugar to a chemical or biofuel.

公开(公告)号：US20170166902A1

公开(公告)日：2017-06-15

申请号：US15376489

申请日：2016-12-12

申请人： The Regents of the University of California

发明人： James C. Liao ,  Igor Bogorad

IPC分类号： C12N15/52 ,  C12N9/88 ,  C12P9/00 ,  C12N15/70

CPC分类号： C12N15/52 ,  C12N9/88 ,  C12N15/63 ,  C12N15/70 ,  C12P9/00 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02P20/52

摘要： Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.

公开(公告)号：US09249430B2

公开(公告)日：2016-02-02

申请号：US14232011

申请日：2012-07-12

申请人： Philippe Marliere

发明人： Philippe Marliere

IPC分类号： C12N15/63 ,  C12N9/16 ,  C12N9/88 ,  C12P7/28 ,  C12P19/32 ,  C12P5/02

CPC分类号： C12P7/28 ,  C12N9/16 ,  C12N9/88 ,  C12N15/63 ,  C12P5/026 ,  C12P17/182 ,  C12P19/32 ,  C12Y301/03011 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02P20/52

摘要： Described are recombinant microorganisms characterized by having phosphoketolase activity, having a diminished or inactivated Embden-Meyerhof-Parnas pathway (EMPP) by inactivation of the gene(s) encoding phosphofructokinase or by reducing phosphofructokinase activity as compared to a non-modified microorganism and having a diminished or inactivated oxidative branch of the pentose phosphate pathway (PPP) by inactivation of the gene(s) encoding glucose-6-phosphate dehydrogenase or by reducing glucose-6-phosphate dehydrogenase activity as compared to a non-modified microorganism. These microorganisms can be used for the production of useful metabolites such as acetone, isobutene or propene.

摘要翻译： 描述的是具有磷酸酮醇酯酶活性的重组微生物，与未修饰的微生物相比，通过灭活编码磷酸果糖激酶的基因或通过减少磷酸果糖激酶活性而具有减少或失活的Embden-Meyerhof-Parnas途径（EMPP） 通过灭活编码葡萄糖-6-磷酸脱氢酶的基因或通过降低葡萄糖-6-磷酸脱氢酶活性与未修饰的微生物相比，戊糖磷酸途径（PPP）的减少或失活的氧化分支。 这些微生物可用于生产有用的代谢物如丙酮，异丁烯或丙烯。

公开(公告)号：US10006033B2

公开(公告)日：2018-06-26

申请号：US14853946

申请日：2015-09-14

申请人： The Regents of the University of California

发明人： James C. Liao ,  Igor Bogorad

IPC分类号： C12P7/16 ,  C12N1/20 ,  C12P7/18 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C07K1/00 ,  C12N15/52 ,  C12P7/06 ,  C12N9/10 ,  C12N9/88

CPC分类号： C12N15/52 ,  C12N9/1022 ,  C12N9/88 ,  C12P7/06 ,  C12P7/16 ,  C12Y202/01003 ,  C12Y401/02009 ,  C12Y401/02022 ,  C12Y401/02043 ,  Y02E50/10 ,  Y02E50/17

摘要： Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.

公开(公告)号：US20140248669A1

公开(公告)日：2014-09-04

申请号：US14232011

申请日：2012-07-12

申请人： Philippe Marliere

发明人： Philippe Marliere

IPC分类号： C12P5/02 ,  C12P19/32 ,  C12P7/28 ,  C12N15/63

CPC分类号： C12P7/28 ,  C12N9/16 ,  C12N9/88 ,  C12N15/63 ,  C12P5/026 ,  C12P17/182 ,  C12P19/32 ,  C12Y301/03011 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02P20/52

摘要： Described are recombinant microorganisms characterized by having phosphoketolase activity, having a diminished or inactivated Embden-Meyerhof-Parnas pathway (EMPP) by inactivation of the gene(s) encoding phosphofructokinase or by reducing phosphofructokinase activity as compared to a non-modified microorganism and having a diminished or inactivated oxidative branch of the pentose phosphate pathway (PPP) by inactivation of the gene(s) encoding glucose-6-phosphate dehydrogenase or by reducing glucose-6-phosphate dehydrogenase activity as compared to a non-modified microorganism. These microorganisms can be used for the production of useful metabolites such as acetone, isobutene or propene.

摘要翻译： 描述的是具有磷酸酮醇酯酶活性的重组微生物，与未修饰的微生物相比，通过灭活编码磷酸果糖激酶的基因或通过减少磷酸果糖激酶活性而具有减少或失活的Embden-Meyerhof-Parnas途径（EMPP） 通过灭活编码葡萄糖-6-磷酸脱氢酶的基因或通过降低葡萄糖-6-磷酸脱氢酶活性与未修饰的微生物相比，戊糖磷酸途径（PPP）的减少或失活的氧化分支。 这些微生物可用于生产有用的代谢物如丙酮，异丁烯或丙烯。

公开(公告)号：US20130157321A1

公开(公告)日：2013-06-20

申请号：US13774153

申请日：2013-02-22

申请人： Ajinomoto Co., Inc.

发明人： Yury Ivanovich Kozlov ,  Akito Chinen ,  Hiroshi Izui ,  Yoshihiko Hara ,  Hisashi Yasueda ,  Konstantin Vyacheslavovich Rybak ,  Ekaterina Aleksandrovna Slivinskaya ,  Joanna Yosifovna Katashkina

IPC分类号： C12P13/24 ,  C12P13/14 ,  C12P7/46 ,  C12P13/06 ,  C12P7/44 ,  C12P13/20 ,  C12P13/12

CPC分类号： C12P13/24 ,  C12N9/88 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/06 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12Y401/02009 ,  C12Y401/02022

摘要： The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

摘要翻译： 本发明提供一种具有生产乙酰辅酶A的有用代谢物的能力，例如L-谷氨酸，L-谷氨酰胺，L-脯氨酸，L-精氨酸，L-亮氨酸，L-半胱氨酸，琥珀酸酯 和聚羟基丁酸酯，其中所述细菌被修饰，使得D-木酮糖-5-磷酸磷酸酮糖苷酶和/或果糖-6-磷酸磷酸酮醇酶的活性增强。 本发明还提供了使用该细菌生产有用代谢物的方法。