公开(公告)号：US20220065890A1

公开(公告)日：2022-03-03

申请号：US17524840

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Albert L. Carrillo ,  John Donohue

IPC: G01N35/10 ,  G01N35/00 ,  B01L3/00

Abstract: Various embodiments of a fluidic system of the present teachings are configured to execute a sequence of fluidic operations over the course of a next generation sequencing analysis for the sequential delivery of various solutions used over the course of analysis to a multilane sensor device. Exemplary fluidic operations include washing, priming and nucleotide reagent delivery through a fluidic multiplexer block that is configured to provide independent fluid distribution to each lane of a multilane sensor device used for detection during an analysis. Accordingly, any number or combination of lanes can be used during an analysis, so that during an analysis one lane in any position can be used singly during a run, all four lanes can be used simultaneously during a run, or any combination of lanes can be used simultaneously during a run.

公开(公告)号：US11169111B2

公开(公告)日：2021-11-09

申请号：US16362367

申请日：2019-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Todd Rearick ,  John Donohue

IPC: G01N27/414 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US10416112B2

公开(公告)日：2019-09-17

申请号：US14853026

申请日：2015-09-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Todd Rearick ,  John Donohue

IPC: C12Q1/68 ,  G06F19/22 ,  G01N27/414

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US12140559B2

公开(公告)日：2024-11-12

申请号：US18145688

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Donohue ,  Daniel Beacham

IPC: G01N27/414 ,  C09D189/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407 ,  G01N33/483

Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

公开(公告)号：US11567036B2

公开(公告)日：2023-01-31

申请号：US16568133

申请日：2019-09-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Donohue ,  Daniel Beacham

IPC: G01N27/414 ,  C09D189/00 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407 ,  G01N33/483

Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

公开(公告)号：US12050196B2

公开(公告)日：2024-07-30

申请号：US17499676

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew Wong ,  Todd Rearick ,  John Donohue

IPC: G01N27/414 ,  G16B30/00

CPC classification number: G01N27/4145 ,  G16B30/00

Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

公开(公告)号：US20200088676A1

公开(公告)日：2020-03-19

申请号：US16568133

申请日：2019-09-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang Hinz ,  John Donohue ,  Daniel Beacham

IPC: G01N27/414 ,  G01N27/28 ,  G01N27/30 ,  G01N27/407 ,  C09D189/00

Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.