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    Oligonucleotides and methods for the preparation of RNA libraries
    2.
    发明授权
    Oligonucleotides and methods for the preparation of RNA libraries 有权

    公开(公告)号：US11136616B2

    公开(公告)日：2021-10-05

    申请号：US15950921

    申请日：2018-04-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jian Gu ,  Kelli Bramlett ,  Christopher Burnett

    IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6848

    Abstract: Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation process. The disclosure provides blocking oligonucleotides which bind to byproduct nucleic acid molecules formed during the ligation of adapters to nucleic acid segments prior to sequencing and inhibit or block amplification of the byproduct nucleic acid molecules in subsequent amplification reactions. Methods for library preparation using blocking oligonucleotides are also provided.

    OLIGONUCLEOTIDES AND METHODS FOR THE PREPARATION OF RNA LIBRARIES
    3.
    发明申请
    OLIGONUCLEOTIDES AND METHODS FOR THE PREPARATION OF RNA LIBRARIES 有权
    寡核苷酸和制备RNA文库的方法

    公开(公告)号：US20140128291A1

    公开(公告)日：2014-05-08

    申请号：US13774648

    申请日：2013-02-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jian GU ,  Kelli Bramlett ,  Christopher Burnett

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6848 ,  C12Q2521/501 ,  C12Q2525/113 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2537/163

    Abstract: Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation process. The disclosure provides blocking oligonucleotides which bind to byproduct nucleic acid molecules formed during the ligation of adapters to nucleic acid segments prior to sequencing and inhibit or block amplification of the byproduct nucleic acid molecules in subsequent amplification reactions. Methods for library preparation using blocking oligonucleotides are also provided.

    Abstract translation: 公开了用于制备用于测序，基因表达谱，微阵列和其他用途的RNA文库以及简化文库制备过程的组合物和方法。 本公开内容提供了阻断寡核苷酸，其结合在连接核酸片段的衔接子之前形成的副产物核酸分子，并在随后的扩增反应中抑制或阻断副产物核酸分子的扩增。 还提供了使用封闭寡核苷酸的文库制备方法。

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