公开(公告)号：US20150056621A1

公开(公告)日：2015-02-26

申请号：US14477293

申请日：2014-09-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lu WANG ,  David Dinauer

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6876 ,  C12Q2600/16

Abstract: Embodiments of the present invention describe primer pairs, methods and kits for identifying and/or detecting killer-cell immunoglobulin-like receptor (KIR) alleles. The present primer sets include one or more primer pairs that can produce amplicons specific for an individual KIR allele and that are less than 1000 bp in size. Additionally, the primer sets can target intra-exon and/or extracellular domains of KIR alleles for amplification.

Abstract translation: 本发明的实施方案描述了用于鉴定和/或检测杀伤细胞免疫球蛋白样受体（KIR）等位基因的引物对，方法和试剂盒。 本发明的引物组包括一个或多个引物对，其可以产生对个体KIR等位基因特异性且小于1000bp的扩增子。 此外，引物组可以靶向用于扩增的KIR等位基因的外显子和/或细胞外结构域。

公开(公告)号：US09885083B2

公开(公告)日：2018-02-06

申请号：US14410886

申请日：2013-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Giorda

IPC: C12Q1/68 ,  G01N33/543 ,  G01N33/569 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

公开(公告)号：US10914735B2

公开(公告)日：2021-02-09

申请号：US15846035

申请日：2017-12-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Giorda

IPC: G01N33/543 ,  G01N33/569 ,  G01N35/00 ,  C12Q1/6876 ,  G01N33/68

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

公开(公告)号：US20180100194A1

公开(公告)日：2018-04-12

申请号：US15846035

申请日：2017-12-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Giorda

IPC: C12Q1/6876 ,  G01N33/543 ,  G01N33/569 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

公开(公告)号：US20150203912A1

公开(公告)日：2015-07-23

申请号：US14410886

申请日：2013-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Glorda

IPC: C12Q1/68 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

Abstract translation: 因此，在一些实施例中，提供了用于检测一个或多个样品中的分析物或分析物的方法。 所述方法包括提供具有可寻址标记和相关配体的固体支持物，使固体基质接触样品，从而形成接触的固体支持物，将接触的固体支持物与FET阵列相关联并检测FET阵列的电特性， 从而检测一个或多个样品中的分析物或分析物。 在其他实施例中，样本包含第二可寻址标记。