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    Bi-directional sequencing compositions and methods
    3.
    发明授权
    Bi-directional sequencing compositions and methods 有权
    双向测序组合物和方法

    公开(公告)号：US09587269B2

    公开(公告)日：2017-03-07

    申请号：US14832772

    申请日：2015-08-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

    IPC: C12P19/34 ,  C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    Abstract translation: 在一些实施方案中，从与支持物连接的核酸模板获得序列信息的方法包括将第一引物与连接于载体的模板链杂交，对核酸模板的一部分进行测序，从而形成延伸的第一引物产物， 与一部分核酸模板互补。在一些实施方案中，该方法还包括将切口引入与延伸的第一引物产物杂交的模板链的一部分中，使用下述方法从模板中降解模板链的一部分： 其中一部分延伸的第一引物保留与模板链的未降解部分杂交，并通过合成测序延伸的第一引物的至少一些单链部分。

    Bi-directional sequencing compositions and methods
    5.
    发明授权
    Bi-directional sequencing compositions and methods 有权

    公开(公告)号：US10508306B2

    公开(公告)日：2019-12-17

    申请号：US16123823

    申请日：2018-09-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason Myers ,  Zhoutao Chen ,  Devin Dressman ,  Theo Nikiforov

    IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

    Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

    PROTEIN DETECTION USING FET
    9.
    发明申请
    PROTEIN DETECTION USING FET 有权
    蛋白质检测使用FET

    公开(公告)号：US20150203912A1

    公开(公告)日：2015-07-23

    申请号：US14410886

    申请日：2013-06-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Scott Conradson ,  David Dinauer ,  Bin Zhao ,  Dmitriy Gremyachinskiy ,  Jason Myers ,  Jeffrey Rossio ,  Victoria Singer ,  Kristina Glorda

    IPC: C12Q1/68 ,  G01N33/68

    CPC classification number: C12Q1/6876 ,  G01N33/543 ,  G01N33/54326 ,  G01N33/54373 ,  G01N33/56977 ,  G01N33/6854 ,  G01N2333/4703

    Abstract: Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

    Abstract translation: 因此，在一些实施例中，提供了用于检测一个或多个样品中的分析物或分析物的方法。 所述方法包括提供具有可寻址标记和相关配体的固体支持物，使固体基质接触样品，从而形成接触的固体支持物，将接触的固体支持物与FET阵列相关联并检测FET阵列的电特性， 从而检测一个或多个样品中的分析物或分析物。 在其他实施例中，样本包含第二可寻址标记。

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