公开(公告)号：US20200340048A1

公开(公告)日：2020-10-29

申请号：US16870398

申请日：2020-05-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20190119739A1

公开(公告)日：2019-04-25

申请号：US16225261

申请日：2018-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q1/6806 ,  C07C229/12 ,  C07D211/60 ,  C07D207/16 ,  C07C229/24

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20200061197A1

公开(公告)日：2020-02-27

申请号：US16557967

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei YANG ,  Parul ANGRISH ,  Xavier de MOLLERAT du JEU ,  Kristin WIEDERHOLT

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US20170073746A1

公开(公告)日：2017-03-16

申请号：US15273824

申请日：2016-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

Abstract translation: 本公开涉及用于各种方法的新型洗涤剂，包括例如核酸扩增反应如聚合酶链式反应（PCR）。 还描述了制备改性洗涤剂的方法。

公开(公告)号：US20200332343A1

公开(公告)日：2020-10-22

申请号：US16866211

申请日：2020-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG

IPC: C12Q1/686 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US20180223334A1

公开(公告)日：2018-08-09

申请号：US15882789

申请日：2018-01-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG

IPC: C12Q1/686 ,  C07D207/16 ,  C12N9/12 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C12N9/96 ,  C07D211/60 ,  C11D1/88

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US20180200374A1

公开(公告)日：2018-07-19

申请号：US15844380

申请日：2017-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei YANG ,  Parul ANGRISH ,  Xavier de MOLLERAT du JEU ,  Kristin WIEDERHOLT

IPC: A61K47/54 ,  C07D211/58 ,  A61K48/00 ,  C12N15/88 ,  C12N15/113 ,  C07D211/28 ,  C07C229/08 ,  C07C227/06

CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US20230212662A1

公开(公告)日：2023-07-06

申请号：US18087735

申请日：2022-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/158

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20140308666A1

公开(公告)日：2014-10-16

申请号：US14250154

申请日：2014-04-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul ANGRISH ,  Zhiwei YANG ,  Jonathan WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

Abstract translation: 本公开涉及用于各种方法的新型洗涤剂，包括例如核酸扩增反应如聚合酶链式反应（PCR）。 还描述了制备改性洗涤剂的方法。