公开(公告)号：US20190300936A1

公开(公告)日：2019-10-03

申请号：US16382294

申请日：2019-04-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob K. FREUDENTHAL ,  Geoffrey DAHLHOFF ,  Kevin MAHER ,  Ming Song CHEN ,  Gary LIM ,  Ronald DANEHY ,  David FORTESCUE ,  Ming SHEN ,  Woon Liang Soh ,  Francis T. CHENG ,  Ruoyun WU ,  Thiam Chye LEE ,  Gary A. CHAPPELL ,  Chee Kiong LIM ,  Jeff DERE ,  Ted E. STRAUB ,  Jorge FONSECA ,  Kuan Moon BOO ,  Soo Yong LAU

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lends can be a bi-convex lens.

公开(公告)号：US20190201891A1

公开(公告)日：2019-07-04

申请号：US16291757

申请日：2019-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael PALLAS ,  Evan FOSTER ,  Jorge FONSECA ,  James NURSE ,  Theodore STRAUB

IPC: B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C12Q1/686

CPC classification number: B01L3/50851 ,  B01L3/50857 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/141 ,  B01L2300/0609 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/18 ,  C12Q1/686 ,  G01N21/6452

Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

公开(公告)号：US20160096175A1

公开(公告)日：2016-04-07

申请号：US14893356

申请日：2014-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael PALLAS ,  Evan FOSTER ,  Jorge FONSECA ,  James NURSE ,  Theodore STRAUB

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  B01L9/00

CPC classification number: B01L3/50851 ,  B01L3/50857 ,  B01L7/52 ,  B01L9/523 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/141 ,  B01L2300/0609 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/18 ,  C12Q1/686 ,  G01N21/6452

Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

Abstract translation: 用于处理多个生物样品的系统包含支撑件和温度控制器。 支撑件被构造成保持壳体，该壳体包括位于内室内的内室和基板，所述基板包含多个包含一个或多个生物样品的分离的反应位点。 温度控制器被配置为在一个或多个生物样品的测定或反应过程中维持或控制至少一种载体，壳体或一种或多种生物样品的温度。 支撑体还被配置为在测定或反应期间相对于水平面保持基板的至少一个表面为正角度。

公开(公告)号：US20150328634A1

公开(公告)日：2015-11-19

申请号：US14443631

申请日：2013-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jorge FONSECA

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L9/523 ,  B01L3/5088 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2300/0609 ,  B01L2300/0654

Abstract: A case for containing biological samples includes a base, a substrate, and a cover. The substrate includes a plurality of reaction regions disposed along a surface of the substrate and configured to receive one or more biological samples. The cover includes a downward facing surface, is configured to be attached to the base to provide a cavity containing the substrate, and is configured so that the downward facing surface of the cover is parallel or approximately parallel to the substrate surface. The cover may comprise a fill port disposed along the inner surface and is configured for at least partially filling the cavity when the cover is attached to the base. The base may comprise a plurality of tabs located on an upward facing surface thereof, wherein the substrate is attached to the base by physical contact between at least some of the tabs and the substrate.

Abstract translation: 用于容纳生物样品的情况包括基底，基底和盖。 衬底包括沿着衬底的表面设置并被配置为接收一个或多个生物样品的多个反应区域。 盖子包括面向下的表面，被配置为附接到基座以提供容纳基底的空腔，并且被构造成使得盖的朝下的表面平行或大致平行于基底表面。 盖可以包括沿着内表面设置的填充口，并且构造成当盖附接到基座时至少部分地填充空腔。 底座可以包括位于其向上表面上的多个突片，其中通过至少一些突片和基底之间的物理接触将基底附接到基底。

公开(公告)号：US20200340041A1

公开(公告)日：2020-10-29

申请号：US16763583

申请日：2018-11-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelly LI ,  Ioanna PAGANI ,  Jisheng LI ,  Sunali PATEL ,  Kamini VARMA ,  Jorge FONSECA ,  Nitin PURI ,  Evan DIAMOND

IPC: C12Q1/689 ,  C12Q1/686 ,  C12Q1/6837

Abstract: Various methods are disclosed for amplifying nucleic acid sequences in a nucleic acid sample. The methods involve forming at least five amplification reaction mixes each including an aliquot from a sample source that includes nucleic acid sequences, using at least five different assays each including a pair of amplification primers, the assays selected from the group of assays in Table 1 and/or targeting the sequences specified in Table 1.

公开(公告)号：US20140017148A1

公开(公告)日：2014-01-16

申请号：US14020390

申请日：2013-09-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ronald DANEHY ,  Jorge FONSECA ,  Jayanthi PRASAD

IPC: B01L3/00

CPC classification number: B01L3/56 ,  B01L3/0275 ,  B01L3/0293 ,  B01L2200/025 ,  B01L2300/161 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/103

Abstract: A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. Each of the fluid dispensers is configured to engage a distal end of a corresponding one of the mandrels. Each sample comprises a solution containing one or more nucleic acid sequences contained within at least one of the fluid dispensers.

Abstract translation: 用于制备生物样品的系统包含主体，其包括近侧和远侧，多个心轴，多个弹性元件，多个流体分配器和一个或多个样品。 心轴可移动地定位在体内，其中每个弹性元件接合相应的一个心轴。 每个流体分配器构造成接合相应的一个心轴的远端。 每个样品包含含有至少一种流体分配器中的一种或多种核酸序列的溶液。