公开(公告)号：US20190064164A1

公开(公告)日：2019-02-28

申请号：US16047504

申请日：2018-07-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Uma LAKSHMIPATHY ,  Rene QUINTANILLA ,  Joanna ASPRER

IPC: G01N33/569 ,  C12N5/0775 ,  C12N5/077 ,  C12N5/00

Abstract: Provided herein are compositions, methods and uses that relate to or result from the identification of markers that can distinguish between cells at different stages of pluripotency. Certain embodiments provide markers that can distinguish between parental cells (i.e. differentiated cells), partially pluripotent (i.e. partially reprogrammed) and pluripotent (i.e. fully reprogrammed cells). Also provided here are uses of such differential markers, for example, in identification of cell potential, in diagnostics, in differential separation, and in creating efficient workflows that involve fewer steps and lesser time in identifying or separating a desired reprogrammed clone or cell line from a mixture of cells at various stages of pluripotency. In certain embodiments, the activity of these markers can be manipulated to influence cell potential for research or medical purposes.

公开(公告)号：US20160290994A1

公开(公告)日：2016-10-06

申请号：US15090529

申请日：2016-04-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Uma LAKSHMIPATHY ,  Upinder SINGH ,  Scott GRECIAN ,  Rene QUINTANILLA ,  Kyle GEE ,  Mahendra RAO

IPC: G01N33/50 ,  G01N1/30 ,  G01N33/58

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

公开(公告)号：US20160010113A1

公开(公告)日：2016-01-14

申请号：US14801757

申请日：2015-07-16

Applicant: Life Technologies Corporation

Inventor： Uma LAKSHMIPATHY ,  Bhaskar Thyagarajan ,  Jonathan Chesnut ,  Vasiliki Anest ,  Robert Bennett ,  Pauline Lieu ,  George Hanson ,  David Thompson ,  Lucas Chase ,  Gary Shipley ,  Elizabeth Wilson

IPC: C12N15/86

CPC classification number: C12N15/86 ,  C12N2710/16221 ,  C12N2710/16222 ,  C12N2710/16243 ,  C12N2799/022 ,  C12N2799/026 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2820/002 ,  C12N2820/007 ,  C12N2820/60

Abstract: The disclosure relates generally to genetic manipulation of stem and primary cells and to reprogramming of somatic cells, more specifically, human cells. In particular, compositions and methods are disclosed for the generation and maintenance of such engineered cells.

Abstract translation: 本公开一般涉及干细胞和原代细胞的遗传操作和体细胞，更具体地，人细胞的重编程。 特别地，公开了用于产生和维护这种工程化细胞的组合物和方法。