公开(公告)号：US12037599B2

公开(公告)日：2024-07-16

申请号：US17001114

申请日：2020-08-24

申请人： LONZA WALKERSVILLE, INC.

发明人： Young Shin ,  Anandita Seth ,  Bingnan Gu

IPC分类号： C12N15/867 ,  C07K14/16 ,  C12N5/071 ,  C12N9/12 ,  C12N15/86

CPC分类号： C12N15/867 ,  C07K14/161 ,  C07K14/162 ,  C07K14/163 ,  C12N5/0686 ,  C12N9/1241 ,  C12N15/8673 ,  C12N15/8676 ,  C12N15/86 ,  C12N2740/15051 ,  C12N2740/16052 ,  C12N2760/20222

摘要： The present disclosure relates to methods for producing lentiviral vector-producing cells. Specifically the methods utilize two plasmids, rather than four, to provide the required packaging elements and transfer vector to a cell, allowing for the production of a large number of lentiviral producer cells, including suspension-based cells, and the production of high amounts of lentivirus. These methods allow for the production of cells that can be later induced to produce lentivirus, and can be tailored to include a specific gene of interest.

公开(公告)号：US11718833B2

公开(公告)日：2023-08-08

申请号：US16720227

申请日：2019-12-19

申请人： LONZA WALKERSVILLE, INC.

发明人： Matthew Hewitt ,  Young Shin ,  Bingnan Gu ,  Caitlin M. Guenther ,  Anandita Seth

IPC分类号： A61K48/00 ,  C12N15/86 ,  C12N7/00 ,  A61K38/00 ,  A61K39/00 ,  C12N15/90 ,  A61K35/76 ,  A61K9/00

CPC分类号： C12N7/00 ,  A61K9/007 ,  A61K9/0019 ,  A61K35/76 ,  C12N15/86 ,  C12N15/90 ,  C12N2740/15043 ,  C12N2740/15051 ,  C12N2750/14143 ,  C12N2750/14151

摘要： The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.