公开(公告)号：US20190085337A1

公开(公告)日：2019-03-21

申请号：US16082188

申请日：2017-03-07

申请人： The University of Toledo

发明人： Terry D. HINDS, JR. ,  Lucien MCBETH

IPC分类号： C12N15/113 ,  A61K47/64 ,  A61K38/16 ,  A61P35/00

CPC分类号： C12N15/1138 ,  A61K38/00 ,  A61K38/162 ,  A61K47/6455 ,  A61P35/00 ,  C07K14/163 ,  C07K14/721 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/113 ,  C12N2310/14 ,  C12N2310/3181 ,  C12N2310/3513 ,  C12Q1/68

摘要： A peptide nucleic acid (PNA) referred to herein as Sweet-P, compositions comprising the methods of making the same, mid methods of using the same, are described.

公开(公告)号：US09969780B1

公开(公告)日：2018-05-15

申请号：US10597926

申请日：2005-03-11

申请人： Barbara Ensoli

发明人： Barbara Ensoli

IPC分类号： C07K14/16 ,  A61K39/21 ,  A61K38/17 ,  C07K14/73

CPC分类号： C07K14/16 ,  A61K38/1774 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/57 ,  A61K2039/622 ,  C07K14/005 ,  C07K14/162 ,  C07K14/163 ,  C07K14/70514 ,  C07K16/1063 ,  C07K16/1072 ,  C07K16/2866 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16322 ,  C12N2740/16334 ,  G01N2469/20

摘要： Complexes comprising HIV Tat and the V3 loop from gp120 Env provide novel epitopes and are immunogenic to prevent or inhibit infection by HIV.

公开(公告)号：US09777042B2

公开(公告)日：2017-10-03

申请号：US13327244

申请日：2011-12-15

申请人： Vincent Craig Bond ,  Michael Powell ,  Ming Bo Huang ,  Syed Ali

发明人： Vincent Craig Bond ,  Michael Powell ,  Ming Bo Huang ,  Syed Ali

IPC分类号： C07K14/005 ,  C07K14/16 ,  G01N33/543 ,  A61K9/127 ,  A61K38/16 ,  A61K39/39 ,  A61K45/06 ,  G01N33/566 ,  C12P21/02 ,  C12N15/00 ,  A61K39/00 ,  A61K38/00

CPC分类号： C07K14/005 ,  A61K9/127 ,  A61K38/00 ,  A61K38/162 ,  A61K39/39 ,  A61K45/06 ,  A61K2039/55555 ,  C07K14/163 ,  C07K2319/055 ,  C07K2319/50 ,  C07K2319/60 ,  C12N15/00 ,  C12N2740/16322 ,  C12P21/02 ,  G01N33/543 ,  G01N33/566

摘要： The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

公开(公告)号：US20150094226A1

公开(公告)日：2015-04-02

申请号：US14504317

申请日：2014-10-01

申请人： Serge BENICHOU ,  Matthias GEYER ,  Sebastian LULF

发明人： Serge BENICHOU ,  Matthias GEYER ,  Sebastian LULF

IPC分类号： C07K16/10 ,  G06F19/16 ,  C12N7/00 ,  G01N33/68 ,  C07K14/005 ,  C12N9/12

CPC分类号： G16B15/00 ,  C07K14/163 ,  C07K16/1045 ,  C07K2299/00 ,  C07K2317/22 ,  C07K2317/34 ,  C07K2317/569 ,  C07K2317/76 ,  C12N9/1205 ,  C12N2740/16322 ,  G01N33/6878 ,  G01N2500/02

摘要： The present invention provides detailed three-dimensional structural information for the complex formed by the Nef protein and the sdAb19 antibody fragment. In addition, the present invention also provides residues which mediate the interaction between the Nef protein and the sdAb19 antibody fragment. The present invention also provides methods for identifying compounds modulating the interaction of the Nef protein and the sdAb19 antibody.

摘要翻译： 本发明提供了由Nef蛋白质和sdAb19抗体片段形成的复合物的详细三维结构信息。 此外，本发明还提供介导Nef蛋白与sdAb19抗体片段之间的相互作用的残基。 本发明还提供鉴定调节Nef蛋白和sdAb19抗体相互作用的化合物的方法。

公开(公告)号：US20130143202A1

公开(公告)日：2013-06-06

申请号：US12977411

申请日：2010-12-23

申请人： Hana Golding ,  Surender Khurana

发明人： Hana Golding ,  Surender Khurana

IPC分类号： G01N33/68 ,  C07K14/16

CPC分类号： G01N33/6854 ,  C07K14/005 ,  C07K14/161 ,  C07K14/162 ,  C07K14/163 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： This invention relates to compositions and methods or the detection of immunodeficiency virus infection, especially immunodeficiency virus-1 (HIV-1) infection. The invention particularly concerns compositions and methods that may be used in HIV vaccine recipients whose sera may contain vaccine-generated anti-HIV-1 antibodies.

公开(公告)号：US20180312548A1

公开(公告)日：2018-11-01

申请号：US16034237

申请日：2018-07-12

申请人： MOREHOUSE SCHOOL OF MEDICINE

发明人： Vincent Craig BOND ,  Michael Powell ,  Ming Bo Huang ,  Syed Ali

IPC分类号： C07K14/005 ,  C12P21/02 ,  A61K38/16 ,  A61K39/39 ,  A61K45/06 ,  G01N33/543 ,  C07K14/16 ,  G01N33/566 ,  A61K9/127 ,  A61K38/00 ,  A61K39/00 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K9/127 ,  A61K38/00 ,  A61K38/162 ,  A61K39/39 ,  A61K45/06 ,  A61K2039/55555 ,  C07K14/163 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/50 ,  C07K2319/60 ,  C12N15/00 ,  C12N2740/16322 ,  C12P21/02 ,  G01N33/543 ,  G01N33/566

摘要： The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

公开(公告)号：US10053496B2

公开(公告)日：2018-08-21

申请号：US15660572

申请日：2017-07-26

申请人： MOREHOUSE SCHOOL OF MEDICINE

发明人： Vincent Craig Bond ,  Michael Powell ,  Ming Bo Huang ,  Syed Ali

IPC分类号： C07K14/05 ,  C07K14/005 ,  C07K14/16 ,  G01N33/543 ,  A61K9/127 ,  A61K38/16 ,  A61K39/39 ,  A61K45/06 ,  G01N33/566 ,  C12P21/02 ,  C12N15/00 ,  A61K39/00 ,  A61K38/00

CPC分类号： C07K14/005 ,  A61K9/127 ,  A61K38/00 ,  A61K38/162 ,  A61K39/39 ,  A61K45/06 ,  A61K2039/55555 ,  C07K14/163 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/50 ,  C07K2319/60 ,  C12N15/00 ,  C12N2740/16322 ,  C12P21/02 ,  G01N33/543 ,  G01N33/566

摘要： The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.

公开(公告)号：US20170119845A1

公开(公告)日：2017-05-04

申请号：US15313940

申请日：2015-05-28

申请人： NoNO Inc.

发明人： Jonathan David Garman

IPC分类号： A61K38/16 ,  A61K47/12 ,  A61K47/26 ,  A61K47/18 ,  C07K14/005 ,  A61K9/19

CPC分类号： A61K38/162 ,  A61K9/0019 ,  A61K9/19 ,  A61K38/00 ,  A61K38/10 ,  A61K47/12 ,  A61K47/183 ,  A61K47/26 ,  C07K7/06 ,  C07K7/08 ,  C07K14/005 ,  C07K14/16 ,  C07K14/163 ,  C07K14/705 ,  C07K19/00 ,  C12N2740/16322

摘要： The present invention provides lyophilized formulations of active agents, particularly of TAT-NR2B9c as chloride salts. TAT-NR2B9c has shown promise for treating stroke, aneurysm, subarachnoid hemorrhage and other neurological or neurotrauniatic conditions. The chloride salt of TAT-NR2B9c shows improved stability compared with the acetate salt form of prior formulations. Formulations of the chloride salt of TAT-NR2B9c are stable at ambient temperature thus facilitating maintenance of supplies of such a formulation in ambulances for administration at the scene of illness or accident or in transit to a hospital.

公开(公告)号：US09428557B2

公开(公告)日：2016-08-30

申请号：US12866714

申请日：2009-02-06

申请人： Barbara Ensoli ,  Mauro Magnani

发明人： Barbara Ensoli ,  Mauro Magnani

IPC分类号： C12P21/02 ,  C07K14/15 ,  A61K35/00 ,  A61K39/21 ,  C07K14/155 ,  C07K14/16 ,  C07K14/005 ,  A61K38/16 ,  A61K39/00

CPC分类号： C07K14/163 ,  A61K38/162 ,  A61K39/00 ,  A61K39/21 ,  C07K14/005 ,  C07K14/15 ,  C07K14/155 ,  C07K14/16 ,  C07K2319/10 ,  C12N2740/16322

摘要： Tat protein produced in bulk culture is inactive when induced at conventional optical densities but can be obtained in biologically active form when induced during the logarithmic growth phase.

摘要翻译： 在常规光密度下诱导时，在本体培养物中产生的Tat蛋白是无活性的，但是当在对数生长阶段中诱导时可以以生物活性形式获得。

公开(公告)号：US08722324B2

公开(公告)日：2014-05-13

申请号：US12977320

申请日：2010-12-23

申请人： Hana Golding ,  Surender Khurana

发明人： Hana Golding ,  Surender Khurana

IPC分类号： C12Q1/70 ,  A61K39/21

CPC分类号： G01N33/6854 ,  C07K14/005 ,  C07K14/161 ,  C07K14/162 ,  C07K14/163 ,  C12N2740/16111 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

摘要： This invention relates to compositions and methods or the detection of human immunodeficiency virus-1 (HIV-1) infection by conducting an immunoassay comprising the steps of: (a) contacting a biological sample containing HIV-1 antibody with a peptide, having an epitope, of one or more of SEQ ID 49-56 to form a peptide-anti-HIV-1 antibody complex; (b) contacting the formed complex with an anti-HIV-1 antibody binding molecule to permit the anti-HIV-1 antibody binding molecule to bind to the anti-HIV-1 antibody of the formed peptide-anti-HIV-1 antibody complex and form an extended complex that is immobilized on a solid support; (c) removing unbound anti-HIV-1 antibody and anti-HIV-1 antibody binding molecule from the extended complex; and (d) determining the presence or concentration of the anti-HIV-1 antibody in the biological sample.

摘要翻译： 本发明涉及通过进行免疫测定的组合物和方法或人类免疫缺陷病毒-1（HIV-1）感染的检测，其包括以下步骤：（a）使含有HIV-1抗体的生物样品与具有表位 的一个或多个SEQ ID NO：49-56以形成肽 - 抗HIV-1抗体复合物; （b）使所形成的复合物与抗HIV-1抗体结合分子接触，以使抗HIV-1抗体结合分子与所形成的肽 - 抗HIV-1抗体复合物的抗HIV-1抗体结合 并形成固定在固体支持物上的扩展复合物; （c）从扩展的复合物中除去未结合的抗HIV-1抗体和抗HIV-1抗体结合分子; 和（d）测定生物样品中抗HIV-1抗体的存在或浓度。