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1.发明申请公开(公告)号：US20150225705A1
公开(公告)日：2015-08-13
申请号：US14424560
申请日：2013-03-15
发明人： John E. McBride , Erin Wiswall , Indraneel Shikhare , Haowen Xu , Naomi Thorngren , Heidi H. Hau , Emily Stonehouse
IPC分类号： C12N9/18 , C12N9/40 , C12N9/42 , C12P7/10 , C12N9/38 , C12P3/00 , C12P7/52 , C12P7/28 , C12P7/16 , C12N9/24 , C12P7/56
CPC分类号： C12N9/18 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/2465 , C12N9/2471 , C12N9/248 , C12N9/2482 , C12N9/2491 , C12N9/2494 , C12P3/00 , C12P7/10 , C12P7/16 , C12P7/28 , C12P7/52 , C12P7/56 , C12P19/02 , C12P19/14 , C12Y301/01006 , C12Y301/01072 , C12Y302/01021 , C12Y302/01037 , C12Y302/01072 , C12Y302/01131 , C12Y302/01139 , Y02E50/16
摘要： The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.
摘要翻译： 本发明提供了一种多组分酶系统，其从硬木中水解半纤维素寡聚体，其可以在例如酿酒酵母（Saccharomyces cerevisiae）的酵母中表达。 在一些实施方案中，本发明提供一系列生物催化剂的工程化，其将该酶系统的组分的表达和分泌与强大的，快速的木糖利用和在工业上相关的工艺条件下的乙醇发酵相结合，用于固结的生物处理。 在一些实施方案中，本发明利用菌株的共培养物，其可以通过在发酵系统中引入另外的酶而实现显着改善的性能。
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公开(公告)号：US09745560B2
公开(公告)日：2017-08-29
申请号：US14424560
申请日：2013-03-15
发明人： John E. McBride , Erin Wiswall , Indraneel Shikhare , Haowen Xu , Naomi Thorngren , Heidi H. Hau , Emily Stonehouse
IPC分类号： C12N1/20 , C12N9/18 , C12P19/02 , C12P19/14 , C12N9/24 , C12P7/10 , C12N9/42 , C12N9/40 , C12N9/38 , C12P3/00 , C12P7/16 , C12P7/28 , C12P7/52 , C12P7/56
CPC分类号： C12N9/18 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/2465 , C12N9/2471 , C12N9/248 , C12N9/2482 , C12N9/2491 , C12N9/2494 , C12P3/00 , C12P7/10 , C12P7/16 , C12P7/28 , C12P7/52 , C12P7/56 , C12P19/02 , C12P19/14 , C12Y301/01006 , C12Y301/01072 , C12Y302/01021 , C12Y302/01037 , C12Y302/01072 , C12Y302/01131 , C12Y302/01139 , Y02E50/16
摘要： The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.
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