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公开(公告)号：US20120309644A1
公开(公告)日：2012-12-06
申请号：US13552356
申请日：2012-07-18
申请人： Lee O. Lomas , Jian Ding , Egisto Boschetti
发明人： Lee O. Lomas , Jian Ding , Egisto Boschetti
IPC分类号： C40B30/04 , G01N27/447 , B01D15/42 , F17D1/00
CPC分类号： G01N30/461 , B01D15/325 , B01D15/362 , B01D15/363 , B01L3/5025 , B01L3/50255 , B01L3/563 , B01L2200/026 , B01L2200/028 , B01L2300/0681 , B01L2300/0829 , B01L2300/087 , B01L2400/0487 , C07K1/34 , G01N27/44704 , G01N27/44795 , G01N30/466 , G01N30/468 , Y10T137/2224 , Y10T137/794 , Y10T137/86187 , Y10T436/255
摘要： The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.
摘要翻译： 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。
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公开(公告)号：US20090179146A1
公开(公告)日：2009-07-16
申请号：US11915151
申请日：2006-01-19
申请人： Lee O. Lomas , Jian Ding , Egisto Boschetti
发明人： Lee O. Lomas , Jian Ding , Egisto Boschetti
CPC分类号： G01N30/461 , B01D15/325 , B01D15/362 , B01D15/363 , B01L3/5025 , B01L3/50255 , B01L3/563 , B01L2200/026 , B01L2200/028 , B01L2300/0681 , B01L2300/0829 , B01L2300/087 , B01L2400/0487 , C07K1/34 , G01N27/44704 , G01N27/44795 , G01N30/466 , G01N30/468 , Y10T137/2224 , Y10T137/794 , Y10T137/86187 , Y10T436/255
摘要： The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.
摘要翻译： 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。
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公开(公告)号：US08491791B2
公开(公告)日：2013-07-23
申请号：US13552356
申请日：2012-07-18
申请人： Lee O. Lomas , Jian Ding , Egisto Boschetti
发明人： Lee O. Lomas , Jian Ding , Egisto Boschetti
CPC分类号： G01N30/461 , B01D15/325 , B01D15/362 , B01D15/363 , B01L3/5025 , B01L3/50255 , B01L3/563 , B01L2200/026 , B01L2200/028 , B01L2300/0681 , B01L2300/0829 , B01L2300/087 , B01L2400/0487 , C07K1/34 , G01N27/44704 , G01N27/44795 , G01N30/466 , G01N30/468 , Y10T137/2224 , Y10T137/794 , Y10T137/86187 , Y10T436/255
摘要： The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.
摘要翻译： 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。
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公开(公告)号：US08246832B2
公开(公告)日：2012-08-21
申请号：US11915151
申请日：2006-01-19
申请人： Lee O. Lomas , Jian Ding , Egisto Boschetti
发明人： Lee O. Lomas , Jian Ding , Egisto Boschetti
IPC分类号： B01D63/00
CPC分类号： G01N30/461 , B01D15/325 , B01D15/362 , B01D15/363 , B01L3/5025 , B01L3/50255 , B01L3/563 , B01L2200/026 , B01L2200/028 , B01L2300/0681 , B01L2300/0829 , B01L2300/087 , B01L2400/0487 , C07K1/34 , G01N27/44704 , G01N27/44795 , G01N30/466 , G01N30/468 , Y10T137/2224 , Y10T137/794 , Y10T137/86187 , Y10T436/255
摘要： The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.
摘要翻译： 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。
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公开(公告)号：US20080023630A1
公开(公告)日：2008-01-31
申请号：US11557286
申请日：2006-11-07
申请人： Egisto Boschetti , Lee O. Lomas
发明人： Egisto Boschetti , Lee O. Lomas
IPC分类号： H01J49/00
CPC分类号： H01J49/0418
摘要： A mass spectrometer probe is formed of a nonconductive polymer that is doped with conductive material. The probe may be used as, or as part of, a repeller plate in a parallel laser ion desorption/ionization time-of-flight mass spectrometer. Transparent locations on the probe enable a sample placed thereon to be visualized before or during mass spectrometry. The conductive nature of the probe maintains the consistency of the electromagnetic field applied to the sample. The probe also displays low outgassing and high mechanical and chemical stability, thereby enabling it to be used repetitively.
摘要翻译： 质谱仪探针由掺杂有导电材料的非导电聚合物形成。 探针可以用作平行激光离子解吸/电离飞行时间质谱仪中的或作为排斥板的一部分。 在探针之前或期间，探针上的透明位置使其上放置的样品可视化。 探头的导电性质保持了施加到样品的电磁场的一致性。 该探头还显示出低的除气和高的机械和化学稳定性，从而使其能够重复使用。
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公开(公告)号：US20110250167A1
公开(公告)日：2011-10-13
申请号：US12822800
申请日：2010-06-24
申请人： Egisto Boschetti , Lee O. Lomas
发明人： Egisto Boschetti , Lee O. Lomas
IPC分类号： A61K38/20 , A61K38/22 , A61K38/18 , A61K39/00 , A61K39/395 , A61K38/28 , A61K38/49 , A61K38/21 , A61P37/02 , A61P35/00 , A61P19/00 , A61P31/12 , A61P17/06 , C40B40/04 , C40B60/12 , A61K38/43
摘要： The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.
摘要翻译： 本发明提供了用于纯化靶蛋白质组的方法和试剂盒。 该方法包括以下步骤：将包含至少95％的目标蛋白质组和至多5％的污染蛋白质的样品与具有不同结合部分的结合部分的文库结合，结合污染蛋白质和少数靶蛋白质组 到结合部分的文库，将未结合的靶蛋白基团与结合到结合部分文库的蛋白质分离并收集未结合的靶蛋白质。 收集的目标蛋白质比样品中的目标蛋白质组更为纯净。
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公开(公告)号：US07815783B2
公开(公告)日：2010-10-19
申请号：US11073999
申请日：2005-03-08
申请人： Egisto Boschetti , Lee O. Lomas , Pierre Girot
发明人： Egisto Boschetti , Lee O. Lomas , Pierre Girot
IPC分类号： G01N27/453 , B01D57/02
CPC分类号： G01N27/44704 , B01D57/02 , B01J20/3204 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J2220/54 , C07K1/24 , C07K1/34 , G01N27/44795
摘要： Each embodiment includes a central sample reservoir and a plurality of satellite reservoirs. In a first embodiment, a first electrode in electrical contact with the central reservoir is charged and second electrodes in electrical contact with the satellite reservoirs are sequentially charged, thereby pI filtering molecules in the central reservoir into the satellite reservoirs. In a second embodiment, the central reservoir is configured to rotate so that molecules in a sample in the central reservoir are centrifugally pI-filtered into the satellite reservoirs. In a third embodiment, first and second electrodes proximate opposite first and second satellite reservoirs, respectively, are charged. Some molecules in a sample are pI filtered into the first and second satellite reservoirs. Third and fourth electrodes proximate opposite third and fourth satellite reservoirs, respectively, are then charged. Some molecules in a sample are pI filtered into the third and fourth satellite reservoirs.
摘要翻译： 每个实施例包括中央样品储存器和多个卫星储存器。 在第一实施例中，与中央储存器电接触的第一电极被充电，并且与卫星储存器电接触的第二电极被顺序地充电，从而将中央储存器中的分子过滤到卫星储存器中。 在第二实施例中，中央储存器被配置为旋转，使得中央储存器中的样品中的分子被离心地pI过滤到卫星储存器中。 在第三实施例中，分别与第一和第二卫星储存器相邻的第一和第二电极被充电。 样品中的一些分子被pI过滤到第一和第二卫星储层中。 然后分别靠近相对的第三和第四卫星储存器的第三和第四电极被充电。 样品中的一些分子被pI过滤到第三和第四卫星储层中。
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公开(公告)号：US07348184B2
公开(公告)日：2008-03-25
申请号：US10626493
申请日：2003-07-23
申请人： William E. Rich , Egisto Boschetti , Lee O. Lomas , Tai-Tung Yip
发明人： William E. Rich , Egisto Boschetti , Lee O. Lomas , Tai-Tung Yip
IPC分类号： G01N33/543
CPC分类号： C40B30/04 , G01N33/54306 , G01N33/6803
摘要： This invention provides methods and materials for mapping interaction characteristics between components of a multicomponent biological complex. The methods involve capturing a multicomponent complex on a solid support and washing the support with a series of elution washes forming a gradient of solute concentrations, and determining whether a particular elution wash eluted a particular component.
摘要翻译： 本发明提供了用于映射多组分生物复合物的组分之间的相互作用特征的方法和材料。 该方法包括在固体支持物上捕获多组分复合物，并用洗脱洗涤洗涤洗脱洗涤洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱洗脱特定组分。
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公开(公告)号：US07754861B2
公开(公告)日：2010-07-13
申请号：US11388181
申请日：2006-03-22
申请人： Egisto Boschetti , Lee O. Lomas
发明人： Egisto Boschetti , Lee O. Lomas
摘要： The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.
摘要翻译： 本发明提供了用于纯化靶蛋白质组的方法和试剂盒。 该方法包括以下步骤：将包含至少95％的目标蛋白质组和至多5％的污染蛋白质的样品与具有不同结合部分的结合部分的文库结合，结合污染蛋白质和少数靶蛋白质组 到结合部分的文库，将未结合的靶蛋白基团与结合到结合部分文库的蛋白质分离并收集未结合的靶蛋白质。 收集的目标蛋白质比样品中的目标蛋白质组更为纯净。
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