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公开(公告)号：US06300112B1
公开(公告)日：2001-10-09
申请号：US08981446
申请日：1997-12-22
申请人： Leendert Hendrik De Graaff , Noël Nicolaas Maria Elisabeth Van Peij , Henriëtta Catharina Van Den Broeck , Jacob Visser
发明人： Leendert Hendrik De Graaff , Noël Nicolaas Maria Elisabeth Van Peij , Henriëtta Catharina Van Den Broeck , Jacob Visser
IPC分类号： C12N914
CPC分类号： C12Y302/01037 , C07K14/38 , C07K14/4705 , C12N9/0006 , C12N9/2437 , C12N9/248 , C12N9/2482 , C12N9/88 , C12N15/1027 , C12N15/63 , C12N15/65 , C12N15/80 , C12Q1/02 , C12Y302/01008 , C12Y302/01055
摘要： A nucleotide sequence is provided which encodes a peptide having &bgr;-xylosidase activity and exhibits at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or hybridises under stringent conditions with a nucleotide sequence shown in SEQ ID NO. 1, or a part thereof having at least 15 nucleotides encoding an amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3. Also provided is a peptide having &bgr;-xylosidase activity and exhibiting at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or a part thereof having at least 8 amino acids shown in SEQ ID NO. 1 and described in SEQ ID NO. 3.
摘要翻译： 提供了编码具有β-木糖苷酶活性的肽并与SEQ ID NO：1所示的氨基酸序列呈现至少30％氨基酸同一性的核苷酸序列。 1并描述于SEQ ID NO。 3或在严格条件下与SEQ ID NO：1所示的核苷酸序列杂交。 1或其部分具有编码SEQ ID NO：1所示氨基酸序列的至少15个核苷酸。 1并描述于SEQ ID NO： 还提供了具有β-木糖苷酶活性的肽，并且与SEQ ID NO：1所示的氨基酸序列呈现至少30％的氨基酸同一性。 1并描述于SEQ ID NO： 3或其部分具有SEQ ID NO：1所示的至少8个氨基酸。 1并描述于SEQ ID NO： 3。
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公开(公告)号：US08389269B2
公开(公告)日：2013-03-05
申请号：US12444760
申请日：2007-10-31
申请人： Cornelis Maria Jacobus Sagt , Cornelis Theodorus Verrips , Walraven Henry Muller , Noël Nicolaas Maria Elisabeth Van Peij
发明人： Cornelis Maria Jacobus Sagt , Cornelis Theodorus Verrips , Walraven Henry Muller , Noël Nicolaas Maria Elisabeth Van Peij
IPC分类号： C12N1/00
CPC分类号： C12P21/00 , C07K14/38 , C12N9/0004 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/2405 , C12N9/90 , C12N15/80
摘要： The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.
摘要翻译： 本发明涉及一种改善丝状真菌细胞分泌目的蛋白质的方法，包括诱导细胞中的表型，所述细胞选自降低的ERAD，不诱导升高的ERAD的升高的UPR，其中 ERAD优选降低。 本发明还涉及包含上述表型的丝状真菌细胞。 本发明还涉及其表达可以在丝状真菌细胞中调节以获得上述表型的多核苷酸和多肽。
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公开(公告)号：US08812247B2
公开(公告)日：2014-08-19
申请号：US12306678
申请日：2007-06-15
摘要： The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimization of single codon fitness and/or codon pair fitness. The invention further relates to nucleic acids comprising a coding sequence for a predetermined amino acid sequence, the coding sequence being optimised with respect to single codon fitness and/or codon pair fitness for a given host in the methods of the invention, to host cells comprising such nucleic acids and to methods for producing polypeptides and other fermentation products in which these host cells are used.
摘要翻译： 本发明涉及用于在给定宿主细胞中表达的蛋白质编码序列的优化方法。 该方法应用遗传算法来优化编码预定氨基酸序列的单个密码子适合度和/或密码子对适应性序列。 在算法中重复生成新的序列变体并随后选择拟合变体，直到变体编码序列达到单个密码子适应度和/或密码子对适应度的最小值。 本发明还涉及一种包括处理器和存储器的计算机，所述处理器被布置为从存储器读取和写入存储器，所述存储器包括数据和指令，所述数据和指令被布置为向处理器提供执行用于优化单个密码子的遗传算法的能力 健身和/或密码子对健身。 本发明还涉及包含用于预定氨基酸序列的编码序列的核酸，所述编码序列针对本发明方法中给定宿主的单个密码子适合度和/或密码子对适应性优化至包含 这种核酸以及用于产生其中使用这些宿主细胞的多肽和其它发酵产物的方法。
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公开(公告)号：US20100093030A1
公开(公告)日：2010-04-15
申请号：US12444760
申请日：2007-10-31
申请人： Cornelis Maria Jacobus Sagt , Cornelis Theodorus Verrips , Walraven Henry Muller , Noël Nicolaas Maria Elisabeth Van Peij
发明人： Cornelis Maria Jacobus Sagt , Cornelis Theodorus Verrips , Walraven Henry Muller , Noël Nicolaas Maria Elisabeth Van Peij
CPC分类号： C12P21/00 , C07K14/38 , C12N9/0004 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/2405 , C12N9/90 , C12N15/80
摘要： The present invention relates to a method to improve the secretion of a protein of interest by a filamentous fungal cell comprising inducing a phenotype in the cell selected from the group consisting of a lowered ERAD, an elevated UPR that does not induce an elevated ERAD, wherein ERAD preferably is lowered. The invention further relates to the filamentous fungal cell comprising the phenotype described above. The invention also relates to polynucleotides and polypeptides whose expression can be modulated in the filamentous fungal cell to obtain the above-described phenotype.
摘要翻译： 本发明涉及一种改善丝状真菌细胞分泌目的蛋白质的方法，包括诱导细胞中的表型，所述细胞选自降低的ERAD，不诱导升高的ERAD的升高的UPR，其中 ERAD优选降低。 本发明还涉及包含上述表型的丝状真菌细胞。 本发明还涉及其表达可以在丝状真菌细胞中调节以获得上述表型的多核苷酸和多肽。
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公开(公告)号：US20090286280A1
公开(公告)日：2009-11-19
申请号：US12306678
申请日：2007-06-15
摘要： The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimisation of single codon fitness and/or codon pair fitness. The invention further relates to nucleic acids comprising a coding sequence for a predetermined amino acid sequence, the coding sequence being optimised with respect to single codon fitness and/or codon pair fitness for a given host in the methods of the invention, to host cells comprising such nucleic acids and to methods for producing polypeptides and other fermentation products in which these host cells are used.
摘要翻译： 本发明涉及用于在给定宿主细胞中表达的蛋白质编码序列的优化方法。 该方法应用遗传算法来优化编码预定氨基酸序列的单个密码子适合度和/或密码子对适应性序列。 在算法中重复生成新的序列变体并随后选择拟合变体，直到变体编码序列达到单个密码子适应度和/或密码子对适应度的最小值。 本发明还涉及一种包括处理器和存储器的计算机，所述处理器被布置为从存储器读取和写入存储器，所述存储器包括数据和指令，其被布置为向处理器提供执行遗传算法以优化单个密码子的能力 健身和/或密码子对健身。 本发明还涉及包含用于预定氨基酸序列的编码序列的核酸，所述编码序列针对本发明方法中给定宿主的单个密码子适合度和/或密码子对适应性优化至包含 这种核酸以及用于产生其中使用这些宿主细胞的多肽和其它发酵产物的方法。
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公开(公告)号：US08211667B2
公开(公告)日：2012-07-03
申请号：US11578514
申请日：2005-04-18
CPC分类号： C07K14/38
摘要： The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
摘要翻译： 本发明涉及分离的真菌启动子DNA序列，DNA构建体，载体和包含这些启动子的真菌宿主细胞，其与编码多肽的编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或产生多肽的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。
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7.发明授权公开(公告)号：US07888489B2
公开(公告)日：2011-02-15
申请号：US11795824
申请日：2006-01-24
申请人： Johannes Andries Roubos , Serge Petrus Donkers , Hein Stam , Noël Nicolaas Maria Elisabeth Van Peij
发明人： Johannes Andries Roubos , Serge Petrus Donkers , Hein Stam , Noël Nicolaas Maria Elisabeth Van Peij
摘要： The invention relates to a nucleotide sequence comprising; a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, the synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally the nucleotide sequence comprises control sequences such as; one translational termination sequence orientated in 5′ towards 3′ direction selected from the following list of sequences; TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5′ towards 3′ direction selected from the following list of sequences; gctnccyyc (SEQ ID NO:20), using ambiguity codes for nucleotides; v (A/C/G); n (A/C/G/T), preferably 5′-GCT TCC TTC-3′ (SEQ ID NO:21). The invention further relates to a consensus translational initiator sequence; 5′-mwChkyCAmv-3′ (SEQ ID NO:16), preferably the translational initiator sequence is selected from the list consisting of; 5′-mwChkyCAAA-3′ (SEQ ID NO:17), 5′-mwChkyCACA-3′ (SEQ ID NO:18), and 5′-mwChkyCAAG-3′ (SEQ ID NO:19).
摘要翻译： 本发明涉及一种核苷酸序列，其包含： 具有优化密码子频率的同义核苷酸编码序列，使得本地密码子已经与同义密码子交换，同义密码子编码与天然密码子相同的氨基酸，并且在表1中定义的密码子使用中的频率高于天然 密码子 并且任选地，所述核苷酸序列包含控制序列，例如 一个位于5'朝向3'方向的平移终止序列，从下列序列列表中选择; TAAG，TAGA和TAAA，优选TAAA，和/或以5'朝向3'方向取向的一个翻译启动子编码序列，其选自以下序列列表; gctnccyyc（SEQ ID NO：20），使用核苷酸的模糊码; v（A / C / G）; n（A / C / G / T），优选5'-GCT TCC TTC-3'（SEQ ID NO：21）。 本发明还涉及一致的翻译起始序列; 5'-mwChkyCAmv-3'（SEQ ID NO：16），优选地，翻译起始子序列选自： 5'-mwChkyCAAA-3'（SEQ ID NO：17），5'-mwChkyCACA-3'（SEQ ID NO：18）和5'-mwChkyCAAG-3'（SEQ ID NO：19）。
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公开(公告)号：US20100112638A1
公开(公告)日：2010-05-06
申请号：US12526289
申请日：2008-02-12
申请人： Cornelis Maria Jacobus Sagt , Noël Nicolaas Maria Elisabeth Van Peij , Herman Abel Bernard Wosten , Alexandra Maria Costa Rodrigues Alves , Ronald Peter De Vries , Ana Marcela Levin Chucrel
发明人： Cornelis Maria Jacobus Sagt , Noël Nicolaas Maria Elisabeth Van Peij , Herman Abel Bernard Wosten , Alexandra Maria Costa Rodrigues Alves , Ronald Peter De Vries , Ana Marcela Levin Chucrel
CPC分类号： C12N15/80 , C12N9/0061 , C12N9/2428 , C12Y110/03002 , C12Y302/01003
摘要： We describe a recombinant host cell for the production of a compound of interest as well as isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding a compound of interest. We also describe methods for expressing a gene of interest and/or producing compounds of interest using a promoter according to the invention.
摘要翻译： 我们描述了用于产生感兴趣的化合物以及分离的真菌启动子DNA序列的重组宿主细胞，包含这些启动子的DNA构建体，载体和真菌宿主细胞，其与编码感兴趣的化合物的编码序列有效结合。 我们还描述了使用根据本发明的启动子表达感兴趣的基因和/或产生感兴趣的化合物的方法。
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公开(公告)号：US08637280B2
公开(公告)日：2014-01-28
申请号：US12094804
申请日：2006-12-01
申请人： Hugo Marc Karel Bauweleers , Dominique Robert Groeseneken , Noël Nicolaas Maria Elisabeth Van Peij
发明人： Hugo Marc Karel Bauweleers , Dominique Robert Groeseneken , Noël Nicolaas Maria Elisabeth Van Peij
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the (bio)synthesis of citric acid. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of citric acid from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for production of citric acid.
摘要翻译： 本发明涉及编码参与柠檬酸（生物）合成的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在从微生物生产柠檬酸中的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其生产柠檬酸的用途。
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公开(公告)号：US20120258489A1
公开(公告)日：2012-10-11
申请号：US13487517
申请日：2012-06-04
CPC分类号： C07K14/38
摘要： The present invention relates to isolated fungal promoter DNA sequences, to DNA constructs, vectors, and fungal host cells comprising these promoters in operative association with coding sequences encoding polypeptides. The present invention also relates to methods for expressing a gene and/or producing a polypeptide using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
摘要翻译： 本发明涉及分离的真菌启动子DNA序列，DNA构建体，载体和包含这些启动子的真菌宿主细胞，其与编码多肽的编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或产生多肽的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。
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