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11 条记录 (耗时 0.045 秒)

    Glial cell line-derived neurotrophic factor
    2.
    发明授权
    Glial cell line-derived neurotrophic factor 失效
    神经胶质细胞系衍生的神经营养因子

    公开(公告)号：US06362319B1

    公开(公告)日：2002-03-26

    申请号：US08452229

    申请日：1995-05-26

    申请人： Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

    发明人： Leu-Fen H. Lin ,  Franklin D. Collins ,  Daniel H. Doherty ,  Jack Lile ,  Susan Bektesh

    IPC分类号： C07K14475

    CPC分类号： A61K9/0085 ,  A61K38/00 ,  C07K14/475 ,  Y10S530/809 ,  Y10S530/839

    摘要： A novel neurotrophic factor referred to as glial derived neurotrophic factor (GDNF) has been identified and isolated from serum free growth conditioned medium of B49 glioblastoma cells. Rat and human genes encoding GDNF have been cloned and sequenced. A gene encoding GDNF has been subcloned into a vector, and the vector has been used to transform a host cell in order to produce biologically active GDNF in a recombinant DNA process.

    摘要翻译： 已经鉴定并分离了一种称为神经胶质神经营养因子（GDNF）的新型神经营养因子，其从B49胶质母细胞瘤细胞的无血清生长条件培养基中分离。 编码GDNF的大鼠和人类基因已被克隆并测序。 编码GDNF的基因已经被亚克隆到载体中，并且该载体已被用于转化宿主细胞，以便在重组DNA过程中产生生物活性的GDNF。

    Purified ciliary neurotrophic factor
    7.
    发明授权
    Purified ciliary neurotrophic factor 失效
    纯化的睫状神经营养因子

    公开(公告)号：US5011914A

    公开(公告)日：1991-04-30

    申请号：US293851

    申请日：1989-01-05

    申请人： Franklin D. Collins ,  Leu-Fen Lin

    发明人： Franklin D. Collins ,  Leu-Fen Lin

    IPC分类号： A61K38/00 ,  C07K14/475 ,  C07K16/22

    CPC分类号： C07K14/475 ,  C07K16/22 ,  A61K38/00

    摘要： A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF (SN-CNFT) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract. The purification is carried out by lowering the pH of the crude nerve extract preparation to form a precipitate which is removed and discarded; raising the pH to about 6.3 followed by ammonium sulfate fractionation; chromatofocusing a solution containing a second precipitate obtained from the 30% to 60% ammonium sulfate containing solution; subjecting the fractions obtained by chromatofocusing to sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE); and performing reversed-phase high-performance liquid chromatography (HPLC) on the SDS-PAGE eluate.Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described.

    摘要翻译： 要求提供睫状神经营养因子（CNTF），特别是坐骨神经CNTF（SN-CNFT）。 本文所述的SN-CNTF是单一蛋白质物质，并且具有比粗提取物增加至大于25,000倍的比活性。 通过降低粗神经提取物制剂的pH以形成除去并丢弃的沉淀物来进行纯化; 将pH升高至约6.3，随后进行硫酸铵分级; 将含有30％至60％含硫酸铵的溶液得到的含有第二沉淀的溶液进行色谱聚焦; 将通过色谱聚焦获得的级分进行十二烷基硫酸钠（SDS） - 聚丙烯酰胺凝胶电泳（PAGE）; 并在SDS-PAGE洗脱液上进行反相高效液相色谱（HPLC）。 还提供了该SN-CNTF的氨基酸数据。 此外，还提供了使用该数据提供SN-CNTF探针和筛选cDNA和基因组文库的方法。 描述了用于生产SN-CNTF的重组DNA方法。

    Purified ciliary neurotrophic factor
    9.
    发明授权
    Purified ciliary neurotrophic factor 失效
    纯化的睫状神经营养因子

    公开(公告)号：US4997929A

    公开(公告)日：1991-03-05

    申请号：US404533

    申请日：1989-09-08

    申请人： Franklin D. Collins ,  Leu-Fen Lin

    发明人： Franklin D. Collins ,  Leu-Fen Lin

    IPC分类号： A61K38/00 ,  C07K14/475 ,  C07K16/22 ,  C12N15/12

    CPC分类号： C07K16/22 ,  C07K14/475 ,  A61K38/00

    摘要： A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF(SN-CNFT) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract.Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described.Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.

    摘要翻译： 要求提供睫状神经营养因子（CNTF），特别是坐骨神经CNTF（SN-CNFT）。 本文所述的SN-CNTF是单一蛋白质物质，并且具有比粗提取物增加至大于25,000倍的比活性。 还提供了该SN-CNTF的氨基酸数据。 此外，还提供了使用该数据提供SN-CNTF探针和筛选cDNA和基因组文库的方法。 描述了用于生产SN-CNTF的重组DNA方法。 提供编码兔和人CNTF的核酸序列。 提供重组表达系统用于生产生物活性CNTF。