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1.发明授权Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms, and the proteins obtained by that expression 失效用于表达前油麻黄素或其各种等位基因和修饰形式或其成熟形式的克鲁维酵母属的酵母，以及通过该表达获得的蛋白质
公开(公告)号：US4657857A
公开(公告)日：1987-04-14
申请号：US570984
申请日：1984-01-18
IPC分类号： C12N15/00 , A61K35/74 , A61K38/00 , C07K14/43 , C12N1/16 , C12N1/19 , C12N9/34 , C12N9/64 , C12N15/09 , C12N15/68 , C12N15/81 , C12N15/90 , C12P21/00 , C12P21/02 , C12R1/645 , C12N1/00
CPC分类号： C12N9/2428 , C07K14/43 , C12N15/68 , C12N15/815 , C12N15/90 , C12N9/6481 , C07K2319/036
摘要： The invention relates to yeasts of the genus Kluyveromyces, in particular K. lactis, acting as hosts for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms, which yeasts contain an rDNA plasmid comprising a structural gene encoding for a thaumatin-like protein, an autonomous replicating sequence derived from K. lactis (KARS), a yeast regulon derived from the GAPDH-genes of S. cerevisiae, and a selection marker, e.g. the trp-1 or the lac-4 gene from S. cerevisiae and K. lactis, respectively. Optionally the plasmid also contains a transcription terminator from a yeast. Further the preparation of such yeasts and the preparation of thaumatin-like proteins with such yeasts and the proteins so obtained are claimed. The yeast described give a better expression than obtained with E. coli as described in EP-PA (A2) 0 054 330 and 0 054 331, published on June 23, 1982.
摘要翻译： PCT No.PCT / EP83 / 00129 Sec。 371日期1984年1月18日 102（e）日期1984年1月18日PCT提交1983年5月19日PCT公布。 公开号WO83 / 04051 日本1983年11月24日。本发明涉及克鲁维酵母属的酵母，特别是乳酸克鲁维酵母作为前油麻黄素表达的宿主或其各种等位基因和修饰形式或其成熟形式，酵母含有rDNA质粒，其包含 编码类似丘脑蛋白的蛋白质的结构基因，来自乳酸克鲁维酵母（KARS）的自主复制序列，源自酿酒酵母的GAPDH基因的酵母调节物，以及选择标记，例如 分别来自酿酒酵母和乳酸克鲁维酵母的trp-1或lac-4基因。 任选地，质粒还含有来自酵母的转录终止子。 此外，要求保护这些酵母的制备以及用这些酵母制备象调蛋白样蛋白质和所得蛋白质。 与EP-PA（A2）0 054 330和0 054 331（1982年6月23日公布）中描述的所述酵母比用大肠杆菌获得的酵母更好。
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公开(公告)号：US20050008751A1
公开(公告)日：2005-01-13
申请号：US10910512
申请日：2004-08-03
申请人： Rene Draaisma , Mettina Koning , Adrianus Ledeboer , Elisabeth Leigh-Firbank , Christianus Van Platerink , Johannes Schalk
发明人： Rene Draaisma , Mettina Koning , Adrianus Ledeboer , Elisabeth Leigh-Firbank , Christianus Van Platerink , Johannes Schalk
CPC分类号： A23C21/026 , A23C9/1234 , A23L33/18 , A23L33/19 , A23V2002/00 , A23Y2220/49 , A23V2250/063 , A23V2250/0654 , A23V2250/0628 , A23V2250/064 , A23V2250/062 , A23V2250/061 , A23V2250/0626 , A23V2250/0624 , A23V2250/0638
摘要： A Fermented milk product is described having an ACE inhibitory effect of at least 35 U/ml, wherein the milk product is produced from milk fermented with Lactobacillus delbrueckii subsp. lactis. Further a food product is described comprising an amount of 0.003 mg/g protein, or more a peptide or peptide salt having 2-15 amino acids, comprising the peptide sequence Asp-Lys and/or Ile-His-Pro-Phe.
摘要翻译： 描述了具有至少35U / ml的ACE抑制作用的发酵乳制品，其中所述乳制品由用德氏乳杆菌酵母发酵的乳制成。 乳酸菌 此外，描述了包含0.003mg / g蛋白质量或更多具有2-15个氨基酸的肽或肽盐的食品，其包含肽序列Asp-Lys和/或Ile-His-Pro-Phe。
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公开(公告)号：US20060246178A1
公开(公告)日：2006-11-02
申请号：US10541477
申请日：2003-12-03
IPC分类号： A23C9/12
CPC分类号： A23C9/1234 , A23C9/1322 , A23Y2220/39
摘要： The invention relates to a fermented milk product comprising an amount of tripeptides VPP and/or IPP expressed as equivalent IPP concentration [IPPeq] of 145 ?M or more comprising 40-600 mmol/kg K+ and/or 30-400 mmol/kg Ca2+ and/or 6-50 mmol/kg Mg2+.
摘要翻译： 本发明涉及一种发酵乳制品，其包含一定量的作为当量IPP浓度[IPPeq]为145〜M或更多的三肽VPP和/或IPP，其含有40-600mmol / kg K +和/或30-400mmol / kg Ca 2+， 和/或6-50mmol / kg Mg2 +。
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