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    METHOD FOR PREPARING CELL-DERIVED VESICLE AND USE THEREOF
    2.
    发明公开
    METHOD FOR PREPARING CELL-DERIVED VESICLE AND USE THEREOF 审中-公开

    公开(公告)号：US20240003792A1

    公开(公告)日：2024-01-04

    申请号：US17928177

    申请日：2021-04-19

    申请人： MDimune Inc.

    发明人： Shin Gyu Bae ,  Seung Wook Oh ,  Dong Woo Han

    IPC分类号： G01N1/40

    CPC分类号： G01N1/4077 ,  G01N2001/4088

    摘要： Provided is a method for preparing cell-derived vesicles, and more particularly, a method for preparing cell-derived vesicles using a cell extruder, and a syringe-type cell extruder for effectively preparing cell-derived vesicles. According to the present invention, by using the method for preparing the cell-derived vesicles and the cell extruder, it is possible to prepare cell-derived vesicles in a stable, economical, and mass-producible manner.

    CELL EXTRUDER AND CELL EXTRUSION METHOD
    3.
    发明申请
    CELL EXTRUDER AND CELL EXTRUSION METHOD 有权

    公开(公告)号：US20230033198A1

    公开(公告)日：2023-02-02

    申请号：US17791850

    申请日：2020-09-22

    申请人： MDimune Inc.

    发明人： Se Hee Kim ,  Ji Eun Lee ,  Shin Gyu Bae ,  Ji Hye Le ,  Jin Hee Park

    IPC分类号： C12M1/33 ,  C12M1/34 ,  G01N1/28 ,  C12M1/00

    摘要： A cell extruder of the present invention comprises: a pressure vessel in which a sample is dispensed; a regulator for adjusting a set pressure of nitrogen gas injected into the pressure vessel and then maintaining a constant pressure; an input valve for opening and closing the injection of nitrogen gas into the pressure vessel; an exhaust valve for removing the internal pressure of the pressure vessel; a filter holder which is provided with a membrane filter soaked with a reagent, so that the sample in the pressure vessel is fed through by the nitrogen gas and crushed into extracellular vesicles; and a collection container in which the extruded sample is stored. According to the present invention, pressure can be freely adjusted by the regulator. That is, the flow rate of the membrane according to pressure can be controlled, thus allowing the validation of a biopharmaceutical production method using an extracellular vesicle extruder. In addition, during cell extrusion, a sintered disc can be removed and cells can be extruded by using only a membrane. Vesicles can be produced within a fine pressure adjustment range (at a fine low pressure at which the membrane is not torn) in which no sintered disc is required.