公开(公告)号：US11993813B2

公开(公告)日：2024-05-28

申请号：US16643161

申请日：2018-07-06

Applicant: MGI TECH CO., LTD.

Inventor： Erkai Liu ,  Wenwei Zhang ,  Ao Chen ,  Chongjun Xu

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q1/6855

CPC classification number: C12Q1/6874 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q2521/307 ,  C12Q2525/197 ,  C12Q2531/137 ,  C12Q2537/157 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/197 ,  C12Q2531/137 ,  C12Q2537/157

Abstract: A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

公开(公告)号：US20230272467A1

公开(公告)日：2023-08-31

申请号：US18144121

申请日：2023-05-05

Applicant: MGI TECH CO., LTD. ,  COMPLETE GENOMICS, INC.

Inventor： Jin Yang ,  Xun Xu ,  Hui Wang ,  Bin Xie ,  Zhuokun Li ,  Shengming Zhao ,  Ao Chen ,  Chongjun Xu ,  Wenwei Zhang ,  Ming Ni

IPC: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/6486 ,  B01L2200/10

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

公开(公告)号：US11692221B2

公开(公告)日：2023-07-04

申请号：US16635131

申请日：2017-08-01

Applicant: MGI TECH CO., LTD. ,  COMPLETE GENOMICS, INC.

Inventor： Jin Yang ,  Xun Xu ,  Hui Wang ,  Bin Xie ,  Zhuokun Li ,  Shengming Zhao ,  Ao Chen ,  Chongjun Xu ,  Wenwei Zhang ,  Ming Ni

IPC: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/6486 ,  B01L2200/10

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

公开(公告)号：US11485966B2

公开(公告)日：2022-11-01

申请号：US16754713

申请日：2017-10-11

Applicant: MGI TECH CO., LTD. ,  COMPLETE GENOMICS, INC.

Inventor： Hui Wang ,  Xun Xu ,  Jin Yang ,  Ao Chen ,  Chongjun Xu ,  Wenwei Zhang

IPC: C12N15/10 ,  C12N15/11 ,  C12Q1/68

Abstract: The present invention provides a method for improving the loading of nucleic acid on a solid support by contacting the solid support with a poloxamer-containing reagent. The present invention also provides a method for improving the stability of a nucleic acid on a solid support, comprising contacting a nucleic acid molecule with a partially double-strand oligonucleotide before or after loading the nucleic acid molecule on a solid support, so as to cause the nucleic acid molecule to hybridize with the oligonucleotide. The present invention also provides a combined use of the two methods.