公开(公告)号：US12104205B2

公开(公告)日：2024-10-01

申请号：US16624782

申请日：2017-06-20

Applicant: MGI Tech Co., Ltd.

Inventor： Lin Yang ,  Haojun Jiang ,  Peng Zeng ,  Xuehan Zhuang ,  Ya Gao ,  Yanyan Zhang ,  Hui Jiang ,  Jing Guo ,  Fang Chen ,  Xun Xu

IPC: C12Q1/68 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6853 ,  C12Q2600/16

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

公开(公告)号：US11739319B2

公开(公告)日：2023-08-29

申请号：US16624779

申请日：2017-06-20

Applicant: MGI TECH CO., LTD.

Inventor： Lin Yang ,  Guodong Huang ,  Ya Gao ,  Yanyan Zhang ,  Haiping Zhang ,  Fang Chen ,  Haojun Jiang ,  Shaohong Zhang ,  Yuqian Wang ,  Yifan Xie ,  Hui Jiang

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1093 ,  C12N15/11

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

公开(公告)号：US11760995B2

公开(公告)日：2023-09-19

申请号：US16624791

申请日：2017-06-20

Applicant: MGI TECH CO., LTD

Inventor： Lin Yang ,  Ya Gao ,  Guodong Huang ,  Yicong Wang ,  Yuqian Wang ,  Yanyan Zhang ,  Fang Chen ,  Na Zhong ,  Hui Jiang ,  Xun Xu

IPC: C12N15/10 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686

CPC classification number: C12N15/1093 ,  C12Q1/686 ,  C12Q1/6853

Abstract: Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3′ end of the first primer, and the first random sequence is located on 5′ end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3′ end of the second primer, and the second random sequence is located on 5′ end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.

公开(公告)号：US20200216874A1

公开(公告)日：2020-07-09

申请号：US16624782

申请日：2017-06-20

Applicant: MGI Tech Co., Ltd.

Inventor： Lin Yang ,  Haojun Jiang ,  Peng Zeng ,  Xuehan Zhuang ,  Ya Gao ,  Yanyan Zhang ,  Hui Jiang ,  Jing Guo ,  Fang Chen ,  Xun Xu

IPC: C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6853

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

公开(公告)号：US20200216838A1

公开(公告)日：2020-07-09

申请号：US16624779

申请日：2017-06-20

Applicant: MGI TECH CO., LTD.

Inventor： Lin Yang ,  Guodong Huang ,  Ya Gao ,  Yanyan Zhang ,  Haiping Zhang ,  Fang Chen ,  Haojun Jiang ,  Shaohong Zhang ,  Yuqian Wang ,  Yifan Xie ,  Hui Jiang

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3′ of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.