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    METHODS FOR ALTERING THE REACTIVITY OF PLANT CELL WALLS
    1.
    发明申请
    METHODS FOR ALTERING THE REACTIVITY OF PLANT CELL WALLS 审中-公开
    改善植物细胞壁蛋白活性的方法

    公开(公告)号：US20130312141A1

    公开(公告)日：2013-11-21

    申请号：US13942204

    申请日：2013-07-15

    申请人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

    发明人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

    IPC分类号： C12N15/82 ,  D02G3/02 ,  C12N9/10

    CPC分类号： C12N15/8246 ,  C12N9/1051 ,  D02G3/02 ,  Y10T428/249921

    摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

    摘要翻译： 提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。 这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

    Methods for altering the reactivity of plant cell walls
    3.
    发明授权
    Methods for altering the reactivity of plant cell walls 有权
    改变植物细胞壁反应性的方法

    公开(公告)号：US08507755B2

    公开(公告)日：2013-08-13

    申请号：US13187378

    申请日：2011-07-20

    申请人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

    发明人： Marc De Block ,  Frank Meulewaeter ,  Rainhard Koch ,  Bernd Essigmann

    IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12N15/8246 ,  C12N9/1051 ,  D02G3/02 ,  Y10T428/249921

    摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.

    摘要翻译： 提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。 这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

    Genetic transformation using a PARP inhibitor
    10.
    发明申请
    Genetic transformation using a PARP inhibitor 审中-公开
    使用PARP抑制剂的遗传转化

    公开(公告)号：US20050014268A1

    公开(公告)日：2005-01-20

    申请号：US10849939

    申请日：2004-05-21

    申请人： Marc De Block

    发明人： Marc De Block

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/55 ,  C12N15/79 ,  C12N15/82 ,  G01N33/50 ,  C12N15/87

    CPC分类号： G01N33/5091 ,  C12N15/79 ,  C12N15/8201 ,  C12N15/8213 ,  C12N15/8289 ,  G01N33/5097

    摘要： The invention concerns a process for producing transgenic plant cells, which comprises: contacting a culture of plant cells with an inhibitor of poly-(ADP-ribose) polymerase, prior to transformation, for a period of time sufficient to reduce the response of the cultured cells to stress and to reduce their metabolism. The untransformed cells are then contacted with foreign DNA comprising at least one gene of interest under conditions in which the foreign DNA is taken up by the untransformed cells and the gene of interest is stably integrated in the nuclear genome of the untransformed cells to produce the transgenic cells. The transgenic plant cells are recovered from the culture. The invention further concerns a process for increasing the frequency of obtaining transgenic plant cells, via Agrobacterium-mediated transformation, which comprises: contacting a culture of plant cells with an inhibitor or poly(ADP-ribose) polymerase prior to transformation for a period of approximately 1 to 2 days or culturing transgenic plant cells after transformation in a medium containing an inhibitor of poly(ADP-ribose) polymerase for a period of time of approximately 1 to 14 days.

    摘要翻译： 本发明涉及一种生产转基因植物细胞的方法，其包括：在转化之前将植物细胞培养物与多聚（ADP-核糖）聚合酶抑制剂接触一段足以减少培养物 细胞应激并减少其新陈代谢。 然后将未转化的细胞与包含至少一种目的基因的外源DNA在外源DNA被未转化细胞摄取的条件下接触，并且目的基因稳定整合到未转化细胞的核基因组中以产生转基因 细胞。 从培养物中回收转基因植物细胞。 本发明还涉及通过农杆菌介导的转化增加获得转基因植物细胞的频率的方法，其包括：在转化前将植物细胞的培养物与抑制剂或聚（ADP-核糖）聚合酶接触约一段时间 1至2天或在含有聚（ADP-核糖）聚合酶抑制剂的培养基中转化后培养转基因植物细胞约1至14天的时间。