公开(公告)号：US5861242A

公开(公告)日：1999-01-19

申请号：US781550

申请日：1997-01-09

申请人： Mark Chee ,  Thomas R. Gingeras ,  Stephen P. A. Fodor ,  Earl A. Hubble ,  MacDonald S. Morris

发明人： Mark Chee ,  Thomas R. Gingeras ,  Stephen P. A. Fodor ,  Earl A. Hubble ,  MacDonald S. Morris

IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C40B40/06 ,  C40B60/14 ,  C12Q1/68

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  C07H21/00 ,  C12Q1/703 ,  B01J2219/00432 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00711 ,  B01J2219/00722 ,  C07B2200/11 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/81

摘要： The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.

摘要翻译： 本发明提供了固定在固体支持物上用于分析人免疫缺陷病毒靶序列的寡核苷酸探针阵列。 该阵列包含至少四组长度为9至21个核苷酸的寡核苷酸探针。 第一探针组具有对应于来自人类免疫缺陷病毒的参考序列中的每个核苷酸的探针。 探针通过与包括相应核苷酸的参考序列的亚序列完全互补而与其对应的核苷酸相关。 因此，每个探针具有被指定为与相应核苷酸的互补核苷酸占据的询问位置的位置。 三个附加的探针组各自具有在第一探针组中的每个探针的相应探针。 因此，对于参考序列中的每个核苷酸，存在四个对应的探针，每个探针组中的一个。 三个附加探针组中的三个相应的探针与来自第一探针的相应探针或其包含询问位置的亚序列相同，除了询问位置被四个相应探针中的每一个中的不同核苷酸占据。