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    Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same
    1.
    发明授权
    Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same 失效
    用于诊断HIV的生物芯片上的核酸探针阵列及其使用方法

    公开(公告)号：US5861242A

    公开(公告)日：1999-01-19

    申请号：US781550

    申请日：1997-01-09

    申请人： Mark Chee ,  Thomas R. Gingeras ,  Stephen P. A. Fodor ,  Earl A. Hubble ,  MacDonald S. Morris

    发明人： Mark Chee ,  Thomas R. Gingeras ,  Stephen P. A. Fodor ,  Earl A. Hubble ,  MacDonald S. Morris

    IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C40B40/06 ,  C40B60/14 ,  C12Q1/68

    CPC分类号： B82Y30/00 ,  B01J19/0046 ,  C07H21/00 ,  C12Q1/703 ,  B01J2219/00432 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00711 ,  B01J2219/00722 ,  C07B2200/11 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/81

    摘要： The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.

    摘要翻译： 本发明提供了固定在固体支持物上用于分析人免疫缺陷病毒靶序列的寡核苷酸探针阵列。 该阵列包含至少四组长度为9至21个核苷酸的寡核苷酸探针。 第一探针组具有对应于来自人类免疫缺陷病毒的参考序列中的每个核苷酸的探针。 探针通过与包括相应核苷酸的参考序列的亚序列完全互补而与其对应的核苷酸相关。 因此，每个探针具有被指定为与相应核苷酸的互补核苷酸占据的询问位置的位置。 三个附加的探针组各自具有在第一探针组中的每个探针的相应探针。 因此，对于参考序列中的每个核苷酸，存在四个对应的探针，每个探针组中的一个。 三个附加探针组中的三个相应的探针与来自第一探针的相应探针或其包含询问位置的亚序列相同，除了询问位置被四个相应探针中的每一个中的不同核苷酸占据。

    Nucleic acid affinity columns
    7.
    发明授权
    Nucleic acid affinity columns 有权
    核酸亲和柱

    公开(公告)号：US06280950B1

    公开(公告)日：2001-08-28

    申请号：US09429521

    申请日：1999-10-28

    申请人： Robert J. Lipshutz ,  MacDonald S. Morris ,  Mark S. Chee ,  Thomas R. Gingeras

    发明人： Robert J. Lipshutz ,  MacDonald S. Morris ,  Mark S. Chee ,  Thomas R. Gingeras

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6806 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6837 ,  C40B40/06 ,  C12Q2565/107 ,  C12Q2525/155

    摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequences; b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.

    摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许同时从样品中选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中， 所述寡核苷酸的密度大于每平方厘米约60个不同的寡核苷酸，并且所有所述不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列; b）扩增所述多重寡核苷酸以提供扩增核酸的库; 和c）将核酸池连接到固体支持物上。

    Nucleic acid affinity columns
    9.
    发明授权
    Nucleic acid affinity columns 有权
    核酸亲和柱

    公开(公告)号：US06828104B2

    公开(公告)日：2004-12-07

    申请号：US10219006

    申请日：2002-08-14

    申请人： Robert J. Lipshutz ,  MacDonald S. Morris ,  Mark S. Chee ,  Thomas R. Gingeras

    发明人： Robert J. Lipshutz ,  MacDonald S. Morris ,  Mark S. Chee ,  Thomas R. Gingeras

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6806 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6837 ,  C40B40/06 ,  C12Q2565/107 ,  C12Q2525/155

    摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequence. b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.

    摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许同时从样品中选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中， 所述寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且所有所述不同寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列。 b）扩增所述多重寡核苷酸以提供扩增核酸的库; 和c）将核酸池连接到固体支持物上。

    Nucleic acid affinity columns
    10.
    发明授权
    Nucleic acid affinity columns 有权
    核酸亲和柱

    公开(公告)号：US06440677B2

    公开(公告)日：2002-08-27

    申请号：US09910223

    申请日：2001-07-20

    申请人： Robert J. Lipshutz ,  MacDonald S. Morris ,  Mark S. Chee ,  Thomas R. Gingeras

    发明人： Robert J. Lipshutz ,  MacDonald S. Morris ,  Mark S. Chee ,  Thomas R. Gingeras

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6806 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6837 ,  C40B40/06 ,  C12Q2565/107 ,  C12Q2525/155

    摘要： Nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of the surface, the density of the oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of the different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequence. b) amplifying the multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.

    摘要翻译： 具有大量不同核酸亲和配体的核酸亲和基质，允许从样品中同时选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在表面的预定区域中， 每1cm 2寡核苷酸的密度大于约60个不同寡核苷酸，并且所有不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列。 b）扩增多重寡核苷酸以提供扩增的核酸库; 和c）将核酸池连接到固体支持物上。