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1.发明授权Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas 失效通过用惰性气体冲洗来延长冷藏红细胞的有效保质期的方法
公开(公告)号：US5624794A
公开(公告)日：1997-04-29
申请号：US473675
申请日：1995-06-05
申请人： Mark W. Bitensky , Tatsuro Yoshida
发明人： Mark W. Bitensky , Tatsuro Yoshida
CPC分类号： A01N1/0226 , A01N1/02
摘要： Method using oxygen removal for extending the useful shelf-life of refrigerated red blood cells. A cost-effective, 4.degree. C. storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. Preservation of adenosine triphosphate levels and reduction in hemolysis and in membrane vesicle production of red blood cells stored at 4.degree. C. for prolonged periods of time is achieved by removing oxygen therefrom at the time of storage; in particular, by flushing with an inert gas. Adenosine triphosphate levels of the stored red blood cells are boosted in some samples by addition of ammonium phosphate.
摘要翻译： 使用除氧方法延长冷藏红细胞的有用保质期的方法。 描述了保持红细胞质量并延长输血后体内存活的成本有效的4℃储存方法。 通过在储存时从其中除去氧气来实现长时间保存在4℃下储存的红细胞溶血和膜囊泡生成中三磷酸腺苷水平的降低和膜囊泡生成。 特别是用惰性气体冲洗。 通过添加磷酸铵在一些样品中促进存储的红细胞的腺苷三磷酸水平。
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公开(公告)号：US06946127B2
公开(公告)日：2005-09-20
申请号：US10211402
申请日：2002-08-02
申请人： Mark W. Bitensky , Tatsuro Yoshida
发明人： Mark W. Bitensky , Tatsuro Yoshida
CPC分类号： A61K35/18 , A61K9/5068 , A61K47/6901
摘要： Disclosed are modified red blood cells which function as deployment platforms for important biomolecules. Such modified red blood cells can confer, for example, in vivo protection against exposure to an otherwise lethal nerve agent.
摘要翻译： 公开了用作重要生物分子的部署平台的改良红细胞。 这种修饰的红细胞可以赋予例如体内保护以免暴露于另外的致死神经药物。
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公开(公告)号：US6162396A
公开(公告)日：2000-12-19
申请号：US847859
申请日：1997-04-26
申请人： Mark W. Bitensky , Tatsuro Yoshida
发明人： Mark W. Bitensky , Tatsuro Yoshida
CPC分类号： A61M1/0209 , A61J1/10
摘要： The present invention relates to a storage device and method for the long-term storage of blood and, more particularly, to a blood storage device and method capable of removing oxygen from the stored blood and thereby prolonging the storage life of the deoxygenated blood.
摘要翻译： 本发明涉及一种用于长期储存血液的储存装置和方法，更具体地涉及一种能够从储存的血液中除去氧气并从而延长脱氧血液的储存寿命的血液储存装置和方法。
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4.发明授权
公开(公告)号：US5789151A
公开(公告)日：1998-08-04
申请号：US857474
申请日：1997-05-15
申请人： Mark W. Bitensky , Tatsuro Yoshida
发明人： Mark W. Bitensky , Tatsuro Yoshida
CPC分类号： A01N1/02 , A01N1/0226 , A61M2202/0429
摘要： Prolonged cold storage of red blood cells by oxygen removal and additive usage. A cost-effective, 4.degree. C. storage procedure that preserves red cell quality and prolongs post-transfusion in vivo survival is described. The improved in vivo survival and the preservation of adenosine triphosphate levels, along with reduction in hemolysis and membrane vesicle production of red blood cells stored at 4.degree. C. for prolonged periods of time, is achieved by reducing the oxygen level therein at the time of storage; in particular, by flushing the cells with an inert gas, and storing them in an aqueous solution which includes adenine, dextrose, mannitol, citrate ion, and dihydrogen phosphate ion, but no sodium chloride, in an oxygen-permeable container which is located in an oxygen-free environment containing oxygen-scavenging materials.
摘要翻译： 通过除氧和添加剂使用长期冷藏红细胞。 描述了保持红细胞质量并延长输血后体内存活的成本有效的4℃储存方法。 通过改善体内存活和三磷酸腺苷水平的保持，以及在4℃下储存的红细胞的溶血和膜囊泡产生减少延长的时间段，可以通过降低其中的氧水平来实现 存储; 特别是通过用惰性气体冲洗细胞并将它们储存在含有腺嘌呤，葡萄糖，甘露糖醇，柠檬酸根离子和磷酸二氢根离子但不含氯化钠的水溶液中，该容器位于 含氧清除材料的无氧环境。
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5.发明授权Cellular diagnostic arrays, methods of using and processing for producing same 有权蜂窝诊断阵列，使用和处理方法来生产它公开(公告)号：US07208120B2
公开(公告)日：2007-04-24
申请号：US09965644
申请日：2001-09-27
CPC分类号： C12M41/46 , B01J2219/00596 , B01J2219/00657 , B01J2219/00659 , B01J2219/00702 , B01J2219/0074 , C12M23/16 , G01N15/1463 , G01N33/54366 , G01N2015/0076 , G01N2015/1486 , G01N2015/1495 , G01N2015/1497
摘要： A cellular diagnostic array has been developed that provides accurate and reproducible measurements of the physical parameters of individual cells en masse, including cell membrane surface area, cell volume, and excess membrane. Three differing patterns are employed. In the first design, a gradient array having rectangular shaped channels is used to capture cells and readily show the ratio of volume to surface area for the captured cell. The second silicone rubber array provided by the present invention employs an array of wedge shaped pipettes, each of which captures an individual cell. The position of the cell within a wedge is enough to rapidly determine the cell's actual surface area and volume. A third type of cellular diagnostic array according to the present invention employs an array of channels designed to deform a cell to study how well the cell responds to repeated deformations.
摘要翻译： 已经开发了一种细胞诊断阵列，其提供了对各个细胞的物理参数的精确和可再现的测量，包括细胞膜表面积，细胞体积和过量的膜。 采用三种不同的模式。 在第一种设计中，使用具有矩形通道的梯度阵列来捕获细胞并且容易地显示捕获的细胞的体积与表面积的比率。 由本发明提供的第二硅橡胶阵列采用楔形移液管阵列，其中每一个捕获单个细胞。 电池在楔形件内的位置足以快速确定电池的实际表面积和体积。 根据本发明的第三类型的细胞诊断阵列使用设计成使细胞变形以研究细胞对重复变形的响应程度的通道阵列。
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6.发明授权Modified red blood cell that has surface molecules that neutralize chemical agents 有权具有中和化学试剂的表面分子的改性红细胞公开(公告)号：US06506381B1
公开(公告)日：2003-01-14
申请号：US09609482
申请日：2000-07-05
申请人： Mark W. Bitensky , Tatsuro Yoshida
发明人： Mark W. Bitensky , Tatsuro Yoshida
IPC分类号： A01N6300
CPC分类号： A61K35/18 , A61K9/5068 , A61K47/6901
摘要： Disclosed are modified red blood cells which function as deployment platforms for important biomolecules. Such modified red blood cells can confer, for example, in vivo protection against exposure to an otherwise lethal nerve agent.
摘要翻译： 公开了用作重要生物分子的部署平台的改良红细胞。 这种修饰的红细胞可以赋予例如体内保护以免暴露于另外的致死神经药物。
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7.发明授权Method using CO for extending the useful shelf-life of refrigerated red blood cells 失效使用CO延长冷藏红细胞有用保质期的方法
公开(公告)号：US5476764A
公开(公告)日：1995-12-19
申请号：US308028
申请日：1994-09-16
申请人： Mark W. Bitensky
发明人： Mark W. Bitensky
IPC分类号： A01N1/02
CPC分类号： A01N1/0226 , A01N1/02
摘要： Method using CO for extending the useful shelf-life of refrigerated red blood cells. Carbon monoxide is utilized for stabilizing hemoglobin in red blood cells to be stored at low temperature. Changes observed in the stored cells are similar to those found in normal red cell aging in the body, the extent thereof being directly related to the duration of refrigerated storage. Changes in cell buoyant density, vesiculation, and the tendency of stored cells to bind autologous IgG antibody directed against polymerized band 3 IgG, all of which are related to red blood cell senescence and increase with refrigerated storage time, have been substantially slowed when red blood cells are treated with CO. Removal of the carbon monoxide from the red blood cells is readily and efficiently accomplished by photolysis in the presence of oxygen so that the stored red blood cells may be safely transfused into a recipient.
摘要翻译： 使用CO延长冷藏红细胞有用保质期的方法。 一氧化碳用于稳定红细胞中的血红蛋白，以在低温下储存。 在储存的细胞中观察到的变化与身体正常红细胞衰老中发现的变化相似，其程度与冷冻储存的持续时间直接相关。 细胞浮力密度变化，囊泡化和存储细胞结合针对聚合带3 IgG的自身IgG抗体的趋势，所有这些都与红细胞衰老有关，并且随着冷冻保存时间的增加而增加，当红血液 用CO处理细胞。通过在氧气存在下通过光解容易且有效地完成从红细胞中去除一氧化碳，使得储存的红细胞可以安全地输入到受体中。
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