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    Bivalent IgY antibody constructs for diagnostic and therapeutic applications
    3.
    发明授权
    Bivalent IgY antibody constructs for diagnostic and therapeutic applications 失效
    用于诊断和治疗应用的二价IgY抗体构建体

    公开(公告)号：US07696323B2

    公开(公告)日：2010-04-13

    申请号：US11510329

    申请日：2006-08-25

    申请人： Reinhard Bredehorst ,  Kerstin Greunke ,  Thomas Grunwald ,  Edzard Spillner

    发明人： Reinhard Bredehorst ,  Kerstin Greunke ,  Thomas Grunwald ,  Edzard Spillner

    IPC分类号： C07K16/00 ,  A61K39/395

    CPC分类号： C07K16/40 ,  C07K16/10 ,  C07K16/462 ,  C07K2317/23 ,  C07K2317/41 ,  C07K2317/622 ,  C07K2317/64

    摘要： This invention relates to the field of recombinant antibody technology. It provides novel recombinant IgY antibody constructs for diagnostic and therapeutical applications. The bivalent antibody constructs display a heterotetrameric or homodimeric format stabilized by disulfide bonds. The constant heavy chain domains CH2-CH4 are partly or completely of avian origin, whereas the VH, VL, CL, and CH1 domains as well as the hinge region may be of avian origin or derived from any other species. The invention allows to combine the advantages of IgY antibodies with those of established mammalian monoclonal antibodies. IgY antibody constructs comprising nonglycosylated IgY constant heavy chain domains allow to reduce unwanted interactions with C-type lectins, e.g., in human sera. Furthermore, chimeric IgY antibody containing mammalian VH, VL, CL, and CH1 domains as well as a mammalian hinge region provide a higher molecular stability than IgY antibodies in acidic conditions and, thereby, are especially suited for peroral therapeutic applications.

    摘要翻译： 本发明涉及重组抗体技术领域。 它提供用于诊断和治疗应用的新型重组IgY抗体构建体。 二价抗体构建物显示通过二硫键稳定的异四聚体或同二聚体形式。 恒定的重链结构域CH2-CH4部分或完全是鸟类来源的，而VH，VL，CL和CH1结构域以及铰链区可以是鸟类来源的或衍生自任何其它物种。 本发明允许将IgY抗体的优点与已建立的哺乳动物单克隆抗体的优点相结合。 包含非糖基化IgY恒定重链结构域的IgY抗体构建体允许减少与C型凝集素的不期望的相互作用，例如在人血清中。 此外，含有哺乳动物VH，VL，CL和CH1结构域以及哺乳动物铰链区的嵌合IgY抗体在酸性条件下提供比IgY抗体更高的分子稳定性，因此特别适用于口服治疗应用。

    Bivalent IgY antibody constructs for diagnostic and therapeutic applications
    4.
    发明申请
    Bivalent IgY antibody constructs for diagnostic and therapeutic applications 失效
    用于诊断和治疗应用的二价IgY抗体构建体

    公开(公告)号：US20070141049A1

    公开(公告)日：2007-06-21

    申请号：US11510329

    申请日：2006-08-25

    申请人： Reinhard Bredehorst ,  Kerstin Greunke ,  Thomas Grunwald ,  Edzard Spillner

    发明人： Reinhard Bredehorst ,  Kerstin Greunke ,  Thomas Grunwald ,  Edzard Spillner

    IPC分类号： A61K39/395 ,  C07K16/44

    CPC分类号： C07K16/40 ,  C07K16/10 ,  C07K16/462 ,  C07K2317/23 ,  C07K2317/41 ,  C07K2317/622 ,  C07K2317/64

    摘要： This invention relates to the field of recombinant antibody technology. It provides novel recombinant IgY antibody constructs for diagnostic and therapeutical applications. The bivalent antibody constructs display a heterotetrameric or homodimeric format stabilized by disulfide bonds. The constant heavy chain domains CH2-CH4 are partly or completely of avian origin, whereas the VH, VL, CL, and CH1 domains as well as the hinge region may be of avian origin or derived from any other species. The invention allows to combine the advantages of IgY antibodies with those of established mammalian monoclonal antibodies. IgY antibody constructs comprising nonglycosylated IgY constant heavy chain domains allow to reduce unwanted interactions with C-type lectins, e.g., in human sera. Furthermore, chimeric IgY antibody containing mammalian VH, VL, CL, and CH1 domains as well as a mammalian hinge region provide a higher molecular stability than IgY antibodies in acidic conditions and, thereby, are especially suited for peroral therapeutic applications.

    摘要翻译： 本发明涉及重组抗体技术领域。 它提供用于诊断和治疗应用的新型重组IgY抗体构建体。 二价抗体构建物显示通过二硫键稳定的异四聚体或同二聚体形式。 恒定的重链结构域C H-2-C H 4部分或完全是鸟类来源的，而V H H， L，C L和C H 1结构域以及铰链区域可以是鸟类来源的或衍生自任何其它物种。 本发明允许将IgY抗体的优点与已建立的哺乳动物单克隆抗体的优点相结合。 包含非糖基化IgY恒定重链结构域的IgY抗体构建体允许减少与C型凝集素的不期望的相互作用，例如在人血清中。 此外，含有哺乳动物V H，V L，C L和C H H 1结构域的嵌合IgY抗体为 以及哺乳动物铰链区域在酸性条件下提供比IgY抗体更高的分子稳定性，因此特别适合于口服治疗应用。

    Method for determining an unknown PNA sequence and uses thereof
    7.
    发明申请
    Method for determining an unknown PNA sequence and uses thereof 审中-公开
    用于确定未知PNA序列的方法及其用途

    公开(公告)号：US20100081801A1

    公开(公告)日：2010-04-01

    申请号：US11582165

    申请日：2006-10-17

    申请人： Reinhard Bredehorst ,  Jorn Glokler ,  Thomas Grunwald ,  Mark Matzas ,  Edzard Spillner

    发明人： Reinhard Bredehorst ,  Jorn Glokler ,  Thomas Grunwald ,  Mark Matzas ,  Edzard Spillner

    IPC分类号： C12Q1/68 ,  C40B30/04 ,  C07H21/04

    CPC分类号： C12Q1/6874 ,  C07K2/00 ,  C12Q1/6869 ,  C12Q2525/107

    摘要： The invention relates to a method for determining an unknown PNA sequence information of PNA molecules of a specific PNA molecule species, wherein, the PNA molecules are contacted with one or several different nucleic acid molecule species comprising nucleic acid molecules with at least one nucleotide, wherein the nucleic acid molecules at least partially comprise a nucleic acid sequence that is complementary to at least a partial sequence of the PNA molecule, wherein nucleic acid molecules having complementary sequences bind to the PNA molecules forming nucleic acid/PNA hybrids, wherein nucleic acid molecules with non-complementary sequences are separated from the nucleic acid/PNA hybrids, wherein thereafter the nucleic acid/PNA hybrids are dissociated into single stranded hybrid nucleic acid molecules and PNA molecules, wherein the single stranded hybrid nucleic acid molecules are subjected to a sequencing process providing hybrid sequence information about the single stranded hybrid nucleic acid sequence, and wherein the hybrid sequence information is optionally translated into the complementary PNA sequence information, and to a method for producing PNA molecules using such a process.

    摘要翻译： 本发明涉及一种用于确定特异性PNA分子物种的PNA分子的未知PNA序列信息的方法，其中所述PNA分子与包含具有至少一个核苷酸的核酸分子的一种或几种不同的核酸分子物质接触，其中 所述核酸分子至少部分地包含与所述PNA分子的至少部分序列互补的核酸序列，其中具有互补序列的核酸分子与形成核酸/ PNA杂交体的PNA分子结合，其中核酸分子与 非互补序列与核酸/ PNA杂交体分离，此后，核酸/ PNA杂交体解离成单链杂交核酸分子和PNA分子，其中单链杂交核酸分子经受测序过程，提供 关于单链杂交体的杂交序列信息 核酸序列，并且其中杂交序列信息任选地翻译成互补的PNA序列信息，以及使用这种方法产生PNA分子的方法。

    Cloning of honey bee allergen
    8.
    发明授权
    Cloning of honey bee allergen 有权
    克隆蜂蜜过敏原

    公开(公告)号：US09446121B2

    公开(公告)日：2016-09-20

    申请号：US12404168

    申请日：2009-03-13

    申请人： Thomas Grunwald

    发明人： Thomas Grunwald

    IPC分类号： A61K38/00 ,  A61K38/04 ,  A61K39/00 ,  A61K39/35 ,  A61K39/36 ,  C07K1/00 ,  C07K2/00 ,  C07K4/00 ,  C07K5/00 ,  C07K7/00 ,  C07K14/00 ,  C07K16/00 ,  C07K14/435

    CPC分类号： A61K39/35 ,  C07K14/43572

    摘要： The present invention relates to a recombinant polypeptide capable of binding to IgE from subjects allergic to venom of an insect from the order Hymenoptera having a homology of more than 70% to the amino acid sequence of SEQ ID NO: 2, which is the honey bee allergen Api m3 (acid phosphatase). The invention further relates to nucleic acid encoding the polypeptide, expression vectors, host cells and methods of preparing the polypeptide, as well as diagnostic and pharmaceutical uses thereof.

    摘要翻译： 本发明涉及一种重组多肽，其能够从与来自蜂巢蜂的SEQ ID NO：2的氨基酸序列具有多于70％同源性的膜翅目昆虫的毒液过敏的受试者结合IgE 过敏原Api m3（酸性磷酸酶）。 本发明还涉及编码多肽，表达载体，宿主细胞的核酸和制备多肽的方法，以及其诊断和药物用途。

    Cloning of honey bee allergen
    10.
    发明授权
    Cloning of honey bee allergen 有权
    克隆蜂蜜过敏原

    公开(公告)号：US07846690B2

    公开(公告)日：2010-12-07

    申请号：US11301329

    申请日：2005-12-13

    申请人： Thomas Grunwald

    发明人： Thomas Grunwald

    IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C07H21/02 ,  C07H21/04

    CPC分类号： A61K39/35 ,  C07K14/43572

    摘要： The present invention relates to a nucleic acid encoding a polypeptide capable of binding to IgE from subjects allergic to venom of an insect from the order Hymenoptera having a homology of more than 70% to the amino acid sequence of SEQ ID NO: 2, which is the honey bee allergen Api m3 (acid phosphatase). The invention further relates to expression vectors, host cells and polypeptides encoded by the nucleic acid, as well as diagnostic and pharmaceutical uses thereof.

    摘要翻译： 本发明涉及一种编码多肽的核酸，该多肽能够与来自与来自SEQ ID NO：2的氨基酸序列具有多于70％同源性的膜翅目昆虫的毒液过敏的受试者的IgE结合， 蜜蜂过敏原Api m3（酸性磷酸酶）。 本发明还涉及由核酸编码的表达载体，宿主细胞和多肽，以及其诊断和药物用途。