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公开(公告)号：US08951764B2
公开(公告)日：2015-02-10
申请号：US13566923
申请日：2012-08-03
申请人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/2428 , C12Y205/0101 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US20130203140A1
公开(公告)日：2013-08-08
申请号：US13566923
申请日：2012-08-03
申请人： Martien H. BERGSMA , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. BERGSMA , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/2428 , C12Y205/0101 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US20120045812A1
公开(公告)日：2012-02-23
申请号：US13204612
申请日：2011-08-05
申请人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung H. Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung H. Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
CPC分类号： C12P5/007 , C12N9/2428 , C12P5/026 , C12P19/02 , C12P19/14 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprene from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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4.发明授权Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges 有权在脱钩条件和/或安全工作范围内生产不含C5烃的异戊二烯的组合物和方法公开(公告)号：US09249070B2
公开(公告)日：2016-02-02
申请号：US13898320
申请日：2013-05-20
申请人： Anthony R. Calabria , Marguerite A. Cervin , Gopal K. Chotani , Richard La Duca , Joseph C. McAuliffe , Michael C. Miller , Timothy A. Sabo , Karl J. Sanford , Erin L. Spring , Gregory M. Whited
发明人： Anthony R. Calabria , Marguerite A. Cervin , Gopal K. Chotani , Richard La Duca , Joseph C. McAuliffe , Michael C. Miller , Timothy A. Sabo , Karl J. Sanford , Erin L. Spring , Gregory M. Whited
CPC分类号： C12P5/007 , C07C11/18 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/75 , C12N15/80 , C12P5/02 , C12Y402/03027 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法，其中固定相中的细胞。 本发明还提供包含这些培养细胞和/或增加量的异戊二烯的组合物。 本发明还提供了在气相中包含不可燃浓度的异戊二烯的体系。 此外，本发明提供了异戊二烯组合物，例如具有增加量的异戊二烯或增加的纯度的组合物。
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5.发明授权Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges 有权在脱钩条件和/或安全工作范围内生产不含C5烃的异戊二烯的组合物和方法公开(公告)号：US08420360B2
公开(公告)日：2013-04-16
申请号：US12496573
申请日：2009-07-01
申请人： Anthony R. Calabria , Marguerite A. Cervin , Gopal K. Chotani , Richard La Duca , Joseph C. McAuliffe , Michael C. Miller , Timothy A. Sabo , Karl J. Sanford , Erin L. Webster , Gregory M. Whited
发明人： Anthony R. Calabria , Marguerite A. Cervin , Gopal K. Chotani , Richard La Duca , Joseph C. McAuliffe , Michael C. Miller , Timothy A. Sabo , Karl J. Sanford , Erin L. Webster , Gregory M. Whited
CPC分类号： C12P5/007 , C07C11/18 , C12N9/88 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/75 , C12N15/80 , C12P5/02 , C12Y402/03027 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.
摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法，其中固定相中的细胞。 本发明还提供包含这些培养细胞和/或增加量的异戊二烯的组合物。 本发明还提供了在气相中包括不可燃浓度的异戊二烯的体系。 此外，本发明提供了异戊二烯组合物，例如具有增加量的异戊二烯或增加的纯度的组合物。
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公开(公告)号：US08778647B2
公开(公告)日：2014-07-15
申请号：US12976572
申请日：2010-12-22
IPC分类号： C12P5/02
CPC分类号： C12P5/007 , Y02E50/343
摘要： The invention provides improved methods for the production of isoprene from biological materials using a membrane bioreactor to culture isoprene-producing cells.
摘要翻译： 本发明提供了使用膜生物反应器从生物材料生产异戊二烯培养异戊二烯生产细胞的改进方法。
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7.发明申请INCREASED ISOPRENE PRODUCTION USING THE ARCHAEAL LOWER MEVALONATE PATHWAY 有权使用ARCHAEAL LOWER MEVALONATE PATHWAY增加的ISOPRENE生产公开(公告)号：US20100086978A1
公开(公告)日：2010-04-08
申请号：US12560390
申请日：2009-09-15
CPC分类号： C12P5/007 , C12N9/1205 , C12N9/88 , C12P9/00 , C12Y207/01036 , C12Y402/03027 , Y02P20/52
摘要： The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
摘要翻译： 本发明的特征在于使用反馈抗性甲羟戊酸激酶多肽（例如古细菌甲羟戊酸激酶多肽）从培养细胞产生异戊二烯的方法。 得到的异戊二烯组合物可以具有增加的异戊二烯的产率和/或纯度。
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8.发明授权公开(公告)号：US08361762B2
公开(公告)日：2013-01-29
申请号：US12560390
申请日：2009-09-15
申请人： Zachary Q. Beck , Anthony R. Calabria , Michael C. Miller , Dmitrii V. Vaviline , Alex T. Nielsen
发明人： Zachary Q. Beck , Anthony R. Calabria , Michael C. Miller , Dmitrii V. Vaviline , Alex T. Nielsen
IPC分类号： C12P9/00
CPC分类号： C12P5/007 , C12N9/1205 , C12N9/88 , C12P9/00 , C12Y207/01036 , C12Y402/03027 , Y02P20/52
摘要： The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
摘要翻译： 本发明的特征在于使用反馈抗性甲羟戊酸激酶多肽（例如古细菌甲羟戊酸激酶多肽）从培养细胞产生异戊二烯的方法。 得到的异戊二烯组合物可以具有增加的异戊二烯的产率和/或纯度。
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