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公开(公告)号：US20130203140A1
公开(公告)日：2013-08-08
申请号：US13566923
申请日：2012-08-03
申请人： Martien H. BERGSMA , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. BERGSMA , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/2428 , C12Y205/0101 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US08951764B2
公开(公告)日：2015-02-10
申请号：US13566923
申请日：2012-08-03
申请人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/2428 , C12Y205/0101 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US20120045812A1
公开(公告)日：2012-02-23
申请号：US13204612
申请日：2011-08-05
申请人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung H. Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. Bergsma , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung H. Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
CPC分类号： C12P5/007 , C12N9/2428 , C12P5/026 , C12P19/02 , C12P19/14 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprene from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US20120034659A1
公开(公告)日：2012-02-09
申请号：US13204561
申请日：2011-08-05
申请人： Martien H. Bergsma , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. Bergsma , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
IPC分类号： C12P19/20 , C12P7/04 , C12P7/16 , C12P7/56 , C12P7/46 , C12P7/18 , C12P13/04 , C12P7/14 , C12P13/14
CPC分类号： C12P7/06 , C12P7/04 , C12P7/14 , C12P7/16 , C12P7/18 , C12P7/46 , C12P7/56 , C12P13/04 , C12P13/14 , C12P19/20 , Y02E50/10 , Y02E50/17
摘要： Embodiments of the present disclosure relate to a process for producing downstream products, such as fermentable sugars and end products, from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产下游产物如可发酵糖和终产物的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US09040279B2
公开(公告)日：2015-05-26
申请号：US12478368
申请日：2009-06-04
摘要： The present disclosure relates to a Bacillus subtilis alpha-amylase (AmyE) or its variants thereof. AmyE or its variants thereof may be used to more efficiently produce fermentable sugars from starch. Also disclosed are a composition comprising a glucoamylase and AmyE or variant thereof and a method of processing starch utilizing the described enzyme composition.
摘要翻译： 本公开涉及枯草芽孢杆菌α-淀粉酶（AmyE）或其变体。 可以使用AmyE或其变体来更有效地从淀粉产生可发酵糖。 还公开了包含葡糖淀粉酶和AmyE或其变体的组合物和利用所述酶组合物加工淀粉的方法。
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公开(公告)号：US08507243B2
公开(公告)日：2013-08-13
申请号：US13119953
申请日：2009-09-11
IPC分类号： C12N9/26
CPC分类号： C12N9/2417 , C11D3/38681 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present invention relates to an alpha-amylase blend, including a B. stearothermophilus alpha-amylase (AmyS) wherein the amino acid at position S242 is substituted and a B. licheniformis alpha-amylase The invention also relates to processes using the alpha-amylase blends for starch liquefaction and saccharification, ethanol production, and a sweetener production.
摘要翻译： 本发明涉及α-淀粉酶共混物，其包括其中取代位置S242的氨基酸和地衣芽孢杆菌α-淀粉酶的嗜热脂肪芽孢杆菌α-淀粉酶（AmyS）本发明还涉及使用α-淀粉酶 用于淀粉液化和糖化，乙醇生产和甜味剂生产的共混物。
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7.发明申请公开(公告)号：US20090215127A1
公开(公告)日：2009-08-27
申请号：US12366333
申请日：2009-02-05
摘要： The present invention relates to a process for producing downstream products, such as fermentable sugars (e.g., glucose) and alcohols (e.g., ethanol) from starch-containing material (e.g., grain) without a pH adjustment before or after the starch liquefaction step.
摘要翻译： 本发明涉及在淀粉液化步骤之前或之后不含pH调节的含淀粉材料（如谷粒）生产下游产物如可发酵糖（如葡萄糖）和醇（如乙醇）的方法。
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公开(公告)号：US20110244526A1
公开(公告)日：2011-10-06
申请号：US13119953
申请日：2009-09-11
CPC分类号： C12N9/2417 , C11D3/38681 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present invention relates to an alpha-amylase blend, including a B. stearothermophilus alpha-amylase (AmyS) wherein the amino acid at position S242 is substituted and a B. licheniformis alpha-amylase blends for starch liquefaction and saccharification, ethanol production, and a sweetener production.
摘要翻译： 本发明涉及一种α-淀粉酶共混物，包括嗜热脂肪芽孢杆菌α-淀粉酶（AmyS），其中取代了S242位的氨基酸和用于淀粉液化和糖化的地衣芽孢杆菌α-淀粉酶混合物，乙醇生产和 甜味剂生产。
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公开(公告)号：US20090252828A1
公开(公告)日：2009-10-08
申请号：US12263886
申请日：2008-11-03
申请人： Luis G. Cascao-Pereira , James T. Kellis, JR. , Bradley A. Paulson , Scott D. Power , Sandra W. Ramer , Vivek Sharma , Andrew Shaw , Jayarama K. Shetty , Donald E. Ward
发明人： Luis G. Cascao-Pereira , James T. Kellis, JR. , Bradley A. Paulson , Scott D. Power , Sandra W. Ramer , Vivek Sharma , Andrew Shaw , Jayarama K. Shetty , Donald E. Ward
CPC分类号： C11D3/38681 , C11D3/386 , C12N9/2417 , D06M16/003
摘要： Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.
摘要翻译： 公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。
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10.发明授权公开(公告)号：US08545907B2
公开(公告)日：2013-10-01
申请号：US13389797
申请日：2010-07-27
摘要： The present disclosure relates to an enzyme blend comprising a low pH, thermostable alpha-amylase and a Bacillus licheniformis alpha-amylase. The blend can include at least about 1.0 Liquefon Unit (LU) of the B. licheniformis alpha-amylase for every 5.0 Modified Wohlgemuth Unit (MWU) of the low pH, thermostable alpha-amylase. The enzyme blend described is suitable for starch liquefaction and saccharification, ethanol production, and/or sweetener production, among other things. Also provided herein is a method of processing a starch by liquefying the starch with the low pH, thermostable alpha-amylase and the Bacillus licheniformis alpha-amylase, simultaneously or sequentially.
摘要翻译： 本公开涉及包含低pH，热稳定性α-淀粉酶和地衣芽孢杆菌α-淀粉酶的酶共混物。 混合物可以包含低pH，热稳定性α-淀粉酶的每5.0个改性Wohlgemuth单位（MWU）的地衣芽孢杆菌α-淀粉酶的至少约1.0Liquefon Unit（LU）。 描述的酶混合物适用于淀粉液化和糖化，乙醇生产和/或甜味剂生产等。 本文还提供了通过用低pH，热稳定性α-淀粉酶和地衣芽孢杆菌α-淀粉酶同时或依次液化淀粉来处理淀粉的方法。
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