公开(公告)号：US08496819B2

公开(公告)日：2013-07-30

申请号：US12146912

申请日：2008-06-26

申请人： Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

发明人： Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

IPC分类号： B01D15/08 ,  G01N30/02 ,  C02F1/28

CPC分类号： B01D15/22 ,  B01J20/282 ,  B01J2220/82 ,  B01J2220/825 ,  G01N30/22 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6069 ,  G01N2030/522 ,  G01N2030/528

摘要： Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using the column are provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

摘要翻译： 提供了具有高耐压性并且即使以小流量进行高速分离和分析的柱和使用该柱的液相色谱装置。 根据本发明的分离柱具有中心的整体式杆，由多孔材料制成，并且具有圆形横截面，填充层布置成使其围绕整体式杆的圆周表面，并且 圆柱形支撑介质布置在填充层外部。 通过在颗粒或珠粒之间填充整体棒和支撑介质之间的管状间隙来形成填充层。

公开(公告)号：US20110303599A1

公开(公告)日：2011-12-15

申请号：US13202722

申请日：2010-03-10

申请人： Norimasa Minamoto ,  Masahito Ito ,  Kisaburo Deguchi ,  Hiroyuki Tanai ,  Kiyotoshi Mori

发明人： Norimasa Minamoto ,  Masahito Ito ,  Kisaburo Deguchi ,  Hiroyuki Tanai ,  Kiyotoshi Mori

IPC分类号： B01D15/08 ,  B01D39/10

CPC分类号： G01N30/603

摘要： In a liquid chromatograph, the disulfide adsorption of iron atoms in a filter for a column is avoided and thus peak tailing in a chromatogram is prevented to thereby prevent a lowering in the sample recovery rate. A liquid chromatograph column comprising a hollow tubular column body, a bead-shaped packing material which is packed in the hollow part of the column body, a filter provided with a mesh which has a mesh size smaller than the particle diameter of the packing material, and a cap which presses the filter against the packing material to thereby enclose the packing material within the column body, characterized in that the main component of the filter is nickel or hastelloy.

摘要翻译： 在液相色谱仪中，避免了用于柱的过滤器中的铁原子的二硫键吸附，因此防止了色谱图中的峰值拖尾，从而防止了样品回收率的降低。 一种液相色谱柱，包括中空管状柱体，填充在柱体中空部分中的珠状包装材料，具有筛网尺寸小于包装材料粒径的筛网的过滤器， 以及将过滤器压靠在包装材料上的盖子，从而将包装材料包围在柱体内，其特征在于，过滤器的主要部件是镍或哈氏合金。

公开(公告)号：US20070199874A1

公开(公告)日：2007-08-30

申请号：US11700844

申请日：2007-02-01

申请人： Masahito Ito ,  Junkichi Miura ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Junkichi Miura ,  Kisaburo Deguchi

IPC分类号： B01D15/08

CPC分类号： G01N30/463 ,  G01N30/461 ,  G01N30/7233 ,  G01N30/96 ,  B01D15/322 ,  B01D15/362 ,  B01D15/325 ,  B01D15/363

摘要： In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.

摘要翻译： 在液相色谱装置中，中间级分离塔另外连接在第一级分离塔和第二级分离塔之间。 优选地，添加用于混合和供给多个溶液的切换单元和液体进料单元以提高分离能力。 可以实现能够避免“溶液干扰”的三维液相色谱仪。 即使是含有亲水性成分和混合状态的疏水性成分的复合物，也可以在线进行分离和分析。

公开(公告)号：US20060271310A1

公开(公告)日：2006-11-30

申请号：US11501750

申请日：2006-08-10

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G01N31/00

CPC分类号： G01N30/8624 ,  G01N30/8631 ,  G01N30/8679 ,  G01N2201/1293

摘要： A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

摘要翻译： 实现了分析多通道色谱图的方法，用于准确地分辨多通道色谱图上的重叠峰，以便分析样品的组成。 首先，指定重叠峰的分量。 确定数据矩阵Dij，并在波长方向上压缩以获得数据矩阵向量Di。 接下来，对二维数据进行解卷积，记录最终标准偏差s0，并将解卷积的二维数据重新卷积到保留强度矩阵。 计算定量光谱强度矩阵，识别组件，并对所识别的组分进行量化。 随后，解决了特征值问题，并且估计了分量的数量n。 迭代执行反褶积和特征值问题的计算，直到峰被隔离。 然后，计算洗脱曲线，识别组分，并对所鉴定的组分进行定量。

公开(公告)号：US06393368B1

公开(公告)日：2002-05-21

申请号：US08811486

申请日：1997-03-05

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G01N3000

CPC分类号： G01N30/8624 ,  G01N30/8631 ,  G01N30/8679 ,  G01N2201/1293

摘要： A method for analyzing a multichannel chromatogram is realized for accurately resolving overlapping peaks on a multichannel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. By compressing three-dimensional data in the wavelength direction, noise components cancel with each other to obtain noise free two-dimensional data Di&bgr;. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. The quantified data is displayed on a screen. Since noise components cancel with each other, it is possible to realize an analyzing method which is capable of accurately resolving and analyzing the components. Also, overlapping peaks are specified, and a data matrix D is determined. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

公开(公告)号：US6148266A

公开(公告)日：2000-11-14

申请号：US605416

申请日：1996-02-22

申请人： Masahito Ito ,  Kisaburo Deguchi ,  Yoshiaki Seki

发明人： Masahito Ito ,  Kisaburo Deguchi ,  Yoshiaki Seki

IPC分类号： G01N30/86 ,  G06F17/10 ,  G06F19/00

CPC分类号： G06F17/10 ,  G01N30/8641

摘要： A method of properly correcting a base line. A concept of flexibility of a base line is introduced as an input index and a gravitation is presumed between the base line and signal data which is measured, thereby constructing a method of correcting the base line which changes gentler than a change in signal with respect to time in a peak area and which is sensitive to an area where a value of the signal data is small. A base line like a natural and smooth curve which isn't easily influenced by a local change in signal such as noise or the like can be set by an input of the flexibility.

公开(公告)号：US5644503A

公开(公告)日：1997-07-01

申请号：US409986

申请日：1995-03-24

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G01N27/447 ,  G01N30/86 ,  G01N31/00 ,  G01N30/00 ,  G06F17/16

CPC分类号： G01N30/8624 ,  G01N2201/1293 ,  G01N30/8631 ,  G01N30/8679

摘要： A method for analyzing a multichannel chromatogram is realized for accurately resolving overlapping peaks on a multichannel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. By compressing three-dimensional data in the wavelength direction, noise components cancel with each other to obtain noise free two-dimensional data Di.beta.. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. The quantified data is displayed on a screen. Since noise components cancel with each other, it is possible to realize an analyzing method which is capable of accurately resolving and analyzing the components. Also, overlapping peaks are specified, and a data matrix D is determined. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

摘要翻译： 实现了用于分析多通道色谱图的方法，用于准确地分辨多通道色谱图上的重叠峰，以允许分析样品的组成。 首先，指定重叠峰的分量。 确定数据矩阵Dij，并在波长方向上压缩以获得数据矩阵向量Di。 通过在波长方向压缩三维数据，噪声分量彼此抵消，以获得无噪声的二维数据Diβ。 接下来，对二维数据进行解卷积，记录最终标准偏差s0，并将解卷积的二维数据重新卷积到保留强度矩阵。 计算定量光谱强度矩阵，识别组件，并对所识别的组分进行量化。 量化的数据显示在屏幕上。 由于噪声分量彼此抵消，因此可以实现能够精确地分析和分析组件的分析方法。 此外，指定重叠的峰值，并且确定数据矩阵D. 随后，解决了特征值问题，估计了组件的数量+ E，uns n + EE。 迭代执行反褶积和特征值问题的计算，直到峰被隔离。 然后，计算洗脱曲线，识别组分，并对所鉴定的组分进行定量。

公开(公告)号：US20100057379A1

公开(公告)日：2010-03-04

申请号：US12611181

申请日：2009-11-03

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G06F19/00 ,  G01N31/00 ,  G06F17/18

CPC分类号： G01N30/8624 ,  G01N30/8631 ,  G01N30/8637 ,  G01N30/8641

摘要： A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

摘要翻译： 提供了一种色谱分析仪，用于利用包含多个重叠峰的色谱图的线性最小二乘法促进曲线拟合。 本发明的特征在于一种色谱数据处理器，用于执行通过使用色谱柱分离待测样品并检测分离的样品而获得的色谱图的数据处理，其中对具有多个样品的任意时间区域中的每个峰值执行拟合处理 从时间区域的前侧或时间区域的背面开始的色谱图的峰值，并且在时间区域中从色谱图中减去处理的峰，使得色谱图中的多个峰可以从 另一个。 因此，仅通过限定一些设定条件，可以容易地将色谱图中的多个重叠峰，特别是三个或更多个重叠峰相互分离。

公开(公告)号：US20050143948A1

公开(公告)日：2005-06-30

申请号：US11061610

申请日：2005-02-22

申请人： Masahito Ito ,  Kisaburo Deguchi

发明人： Masahito Ito ,  Kisaburo Deguchi

IPC分类号： G01N30/86 ,  G01F23/00

CPC分类号： G01N30/8624 ,  G01N30/8631 ,  G01N30/8637 ,  G01N30/8641

摘要： A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. A chromatographic data processor executes fitting processing to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front or back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another.

摘要翻译： 提供了一种色谱分析仪，用于利用包含多个重叠峰的色谱图的线性最小二乘法促进曲线拟合。 色谱数据处理器对从时区的正面或背面开始的具有多个色谱图的峰的任意时间区域中的每个峰值进行拟合处理，并且在时间区域中从色谱图中减去处理的峰，使得 色谱图中的多个峰可以彼此分离。

公开(公告)号：US06314374B1

公开(公告)日：2001-11-06

申请号：US09562026

申请日：2000-05-01

申请人： Masahito Ito ,  Kisaburo Deguchi ,  Yoshiaki Seki

发明人： Masahito Ito ,  Kisaburo Deguchi ,  Yoshiaki Seki

IPC分类号： G06F1900

CPC分类号： G06F17/10 ,  G01N30/8641

摘要： A method of properly correcting a base line. A concept of flexibility of a base line is introduced as an input index and a gravitation is presumed between the base line and signal data which is measured, thereby constructing a method of correcting the base line which changes gentler than a change in signal with respect to time in a peak area and which is sensitive to an area where a value of the signal data is small. A base line like a natural and smooth curve which isn't easily influenced by a local change in signal such as noise or the like can be set by an input of the flexibility.

摘要翻译： 一种正确校正基线的方法。 引入基线的灵活性的概念作为输入索引，并且在基线和被测量的信号数据之间推测引力，由此构建校正基线相对于信号变化更温和的方法，相对于 在峰值区域中的时间，并且其对信号数据的值较小的区域敏感。 可以通过输入灵活性来设定像自然平滑曲线的基线，其不容易受诸如噪声等信号的局部变化的影响。