公开(公告)号：US08191405B2

公开(公告)日：2012-06-05

申请号：US12359381

申请日：2009-01-26

Applicant: Naoya Yasuhara ,  Hironori Kaji ,  Kimihiko Ishii ,  Hiroyuki Wada

Inventor： Naoya Yasuhara ,  Hironori Kaji ,  Kimihiko Ishii ,  Hiroyuki Wada

IPC: G01N1/00

CPC classification number: F04B11/0075 ,  G01N30/32 ,  G01N30/34 ,  G01N2030/324

Abstract: Disclosed herein is a stable solvent delivery device capable of delivering solvent both at high pressure and at constant flow rate. A solvent delivery device comprises a plurality of plungers which reciprocate in the respective pump chambers including an eluent charge side pump chamber and an eluent discharge side pump chamber, a motor to reciprocate these plungers, a control unit to control the operation of the motor, valves which are respectively set at the eluent inlet and outlet of the eluent charge side pump chamber, a first sensor to measure the quantity of load received by the plunger in the eluent charge side pump chamber, and a second sensor to measure the pressure of the eluent discharged from the eluent discharge side pump chamber.

Abstract translation: 本文公开了能够在高压和恒定流速下输送溶剂的稳定的溶剂输送装置。 溶剂输送装置包括在各个泵室中往复运动的多个柱塞，包括洗脱液加料侧泵室和洗脱液排出侧泵室，使这些柱塞往复运动的马达，用于控制马达运转的控制单元 它们分别设置在洗脱液加料侧泵室的洗脱液入口和出口处，第一传感器用于测量柱塞在洗脱液加料侧泵室中接收的负载量，以及第二传感器，用于测量洗脱液的压力 从洗脱液排出侧泵室排出。

公开(公告)号：US07901573B2

公开(公告)日：2011-03-08

申请号：US12081572

申请日：2008-04-17

Applicant: Kimihiko Ishii ,  Hironori Kaji ,  Masahito Ito ,  Yoshihiro Nagaoka ,  Kunihiko Takao

Inventor： Kimihiko Ishii ,  Hironori Kaji ,  Masahito Ito ,  Yoshihiro Nagaoka ,  Kunihiko Takao

IPC: B01D15/08

CPC classification number: B01D15/22 ,  B01D15/206 ,  B01J20/3236 ,  B01J2220/82 ,  B01J2220/86 ,  G01N30/52 ,  G01N30/6017 ,  G01N30/603 ,  G01N30/6047 ,  G01N30/6052 ,  G01N2030/528

Abstract: Disclosed herein is a separation column comprising a porous block covered with a coating wherein space-free adhesion is attainable therebetween by low temperature process without releasing octadecylsilyl bases and others from the porous block. A monolith rod 1 is covered with a metal or resin coating 2. Then, the monolith rod 1 covered by the coating 2 is further covered with a rod retainer 3 made of a low melting point metal (melting point 100-200 degrees C.) to form a column body 19. Since the rod retainer 3 is formed by pressure molding at low temperature (100-200 degrees C.). It is therefore possible to absorb the surface irregularities of the monolith rod 1 by the coating 2 while octadecylsilyl bases and others from are released when the retainer 3 is formed. This allows the potential separation ability of the porous block to be brought out maximally.

Abstract translation: 本文公开了一种分离柱，其包括用涂层覆盖的多孔块，其中通过低温工艺在其间实现无空隙粘合，而不从多孔块中释放十八烷基甲硅烷基等。 整料棒1被金属或树脂涂层2覆盖。然后，用由低熔点金属（熔点100-200℃）制成的棒保持器3进一步覆盖由涂层2覆盖的整料棒1。 以形成柱体19.由于杆保持器3通过在低温（100-200℃）下的模压而形成。 因此，当保持器3形成时，可以通过涂层2吸收整料棒1的表面凹凸，同时十八烷基甲硅烷基等被释放。 这允许最大限度地排出多孔块的潜在分离能力。

公开(公告)号：US20090001007A1

公开(公告)日：2009-01-01

申请号：US12146912

申请日：2008-06-26

Applicant: Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

Inventor： Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

IPC: B01D15/08

CPC classification number: B01D15/22 ,  B01J20/282 ,  B01J2220/82 ,  B01J2220/825 ,  G01N30/22 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6069 ,  G01N2030/522 ,  G01N2030/528

Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using thereof are to be provided.A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

Abstract translation: 提供具有高耐压性并且即使使用小流量也能够进行高速分离和分析的柱和使用其的液相色谱装置。 根据本发明的分离柱具有中心的整体式杆，由多孔材料制成，并且具有圆形横截面，填充层布置成围绕整体式杆的圆周表面，并且 圆柱形支撑介质布置在填充层外部。 通过在颗粒或珠粒之间填充整体棒和支撑介质之间的管状间隙来形成填充层。

公开(公告)号：US20080283458A1

公开(公告)日：2008-11-20

申请号：US12081572

申请日：2008-04-17

Applicant: Kimihiko Ishii ,  Hironori Kaji ,  Masahito Ito ,  Yoshihiro Nagaoka ,  Kunihiko Takao

Inventor： Kimihiko Ishii ,  Hironori Kaji ,  Masahito Ito ,  Yoshihiro Nagaoka ,  Kunihiko Takao

IPC: B01D15/08 ,  B01D15/22

CPC classification number: B01D15/22 ,  B01D15/206 ,  B01J20/3236 ,  B01J2220/82 ,  B01J2220/86 ,  G01N30/52 ,  G01N30/6017 ,  G01N30/603 ,  G01N30/6047 ,  G01N30/6052 ,  G01N2030/528

Abstract: Disclosed herein is a separation column comprising a porous block covered with a coating wherein space-free adhesion is attainable therebetween by low temperature process without releasing octadecylsilyl bases and others from the porous block.A monolith rod 1 is covered with a metal or resin coating 2. Then, the monolith rod 1 covered by the coating 2 is further covered with a rod retainer 3 made of a low melting point metal (melting point 100-200 degrees C.) to form a column body 19. Since the rod retainer 3 is formed by pressure molding at low temperature (100-200 degrees C.). It is therefore possible to absorb the surface irregularities of the monolith rod 1 by the coating 2 while octadecylsilyl bases and others from are released when the retainer 3 is formed. This allows the potential separation ability of the porous block to be brought out maximally.

Abstract translation: 本文公开了一种分离柱，其包括用涂层覆盖的多孔块，其中通过低温工艺在其间实现无空隙粘合，而不从多孔块中释放十八烷基甲硅烷基等。 整料棒1被金属或树脂涂层2覆盖。 然后，用由低熔点金属（熔点为100-200℃）制成的棒保持器3进一步覆盖由涂层2覆盖的整料棒1，以形成柱体19。 由于杆保持器3通过在低温（100-200℃）下进行压力成型而形成。 因此，当保持器3形成时，可以通过涂层2吸收整料棒1的表面凹凸，同时十八烷基甲硅烷基等被释放。 这允许最大限度地排出多孔块的潜在分离能力。

公开(公告)号：US20070084766A1

公开(公告)日：2007-04-19

申请号：US11582455

申请日：2006-10-18

Applicant: Kimihiko Ishii ,  Kiyotoshi Mori ,  Masahito Ito ,  Hironori Kaji ,  Takefumi Yokokura

Inventor： Kimihiko Ishii ,  Kiyotoshi Mori ,  Masahito Ito ,  Hironori Kaji ,  Takefumi Yokokura

IPC: B01D35/14

CPC classification number: G01N30/32 ,  G01N30/34 ,  G01N2030/322 ,  G01N2030/326

Abstract: A pump device is realized, which accurately detects a flow rate of a pump, performs real-time control of the flow rate, and thus can suppress a flow rate error caused by occurrence of pulsation. Control of a pump rotation speed by a pressure sensor has low responsiveness in a low pressure region, and a correlation between a pressure and a flow rate is incomplete in a high flow rate region. With this taken into consideration, a flow rate sensor 10 is disposed at an outlet port 9 of a second pump 4. A position detection sensor 14 detects a point where a first pump 3 is shifted from a suction mode to a discharge mode. Moreover, the flow rate sensor 10 detects that there is no discharge from the pump 3, and that there is discharge from the pump 4 alone. Accordingly, a controller 15 sets a rotation speed of a motor 12 to a speed double the previous speed (double the normal rotation speed). When the flow rate sensor 10 detects an increase in the flow rate at the outlet port 9 of the second pump 4, the controller 15 returns the rotation speed of the motor 12 to a normal rotation speed. The rotation speed of the motor 12 is controlled by accurate flow rate detection by the flow rate sensor 10. Thus, occurrence of pulsation can be suppressed.

Abstract translation: 实现了能够精确地检测泵的流量的泵装置，实现流量的实时控制，能够抑制由脉动发生引起的流量误差。 通过压力传感器控制泵的转速在低压区域的响应性低，在高流量区域中压力与流量的相关性不完全。 考虑到这一点，流量传感器10设置在第二泵4的出口9处。位置检测传感器14检测第一泵3从吸入模式移动到放电模式的点。 此外，流量传感器10检测到没有来自泵3的排出，并且仅从泵4排出。 因此，控制器15将电动机12的转速设定为与先前速度（正常转速两倍）的速度。 当流量传感器10检测到第二泵4的出口9的流量增加时，控制器15将马达12的转速恢复到正常的转速。 通过流量传感器10的精确的流量检测来控制电动机12的转速。因此，可以抑制脉动的发生。

公开(公告)号：US20130161244A1

公开(公告)日：2013-06-27

申请号：US13817658

申请日：2011-07-21

Applicant: Kimihiko Ishii ,  Mitsuhiko Ueda ,  Shouji Tomida

Inventor： Kimihiko Ishii ,  Mitsuhiko Ueda ,  Shouji Tomida

IPC: B01D15/14

CPC classification number: E05B47/026 ,  B65D2221/00 ,  E05B63/0052 ,  E05B2063/0026 ,  G01N35/00 ,  G01N2030/8804 ,  G01N2035/00306

Abstract: Provided is a highly safe chromatography device that does not sacrifice user-friendliness during the addition and replacement of a sample holding container. The chromatography device has doors 10, 11, a door locking mechanism 15, and a sensor 13 for detecting whether the doors 10, 11 are open or closed. The doors 10, 11 are opened and closed to place a sample holding container 6 and a sample rack 1 in the chromatography device. The door locking mechanism 15 is operated to lock the doors 10, 11 when the sensor 13 indicates that the doors 10, 11 are closed and the analysis of a sample in the sample holding container 6 starts, and is operated to unlock the doors 10, 11 when a return operation and a washing operation terminate after a needle 2, a syringe 9, and other mechanical parts operate. Consequently, the sample holding container can be safely added or replaced without having to perform, for instance, a procedure for executing a pause function.

Abstract translation: 提供了一种高度安全的色谱装置，其在添加和更换样品保持容器期间不牺牲用户友好性。 色谱装置具有门10,11，门锁定机构15和用于检测门10,11是否打开或关闭的传感器13。 门10,11打开和关闭以将样品保持容器6和样品架1放置在色谱装置中。 当传感器13指示门10,11关闭并且样品保持容器6中的样品的分析开始并且操作以解锁门10时，门锁定机构15被操作以锁定门10,11， 如图11所示，当针2，注射器9和其他机械部件操作之后返回操作和洗涤操作终止。 因此，可以安全地添加或更换样品保持容器，而不必执行例如执行暂停功能的步骤。

公开(公告)号：US09003868B2

公开(公告)日：2015-04-14

申请号：US13466841

申请日：2012-05-08

Applicant: Yumiko Ono ,  Kimihiko Ishii ,  Eri Yamashita

Inventor： Yumiko Ono ,  Kimihiko Ishii ,  Eri Yamashita

IPC: G01N30/24 ,  G01N30/34 ,  G01N35/10

CPC classification number: G01N30/24 ,  G01N30/34 ,  G01N35/1097

Abstract: Disclosed herein is a sample introducing apparatus which is designed such that the analytical flow path runs from the needle to the separation column without the flow path switching means placed at the downstream side of the needle. This design reduces dead volume, which in turn reduces the diffusion of the sample injected into the analytical flow path. Moreover, the absence of the flow path switching means at the downstream side of the needle to inject a sample into the analytical flow path eliminates connection of the pipe with the flow path switching means. This prevents the sample from remaining in the connecting part, thereby reducing sample carry-over and improving the accuracy of analysis.

Abstract translation: 本文公开了一种样品引入装置，其被设计成使得分析流路从针头延伸到分离柱，而流路切换装置设置在针的下游侧。 该设计减少了死体积，这进而降低了注入分析流路的样品的扩散。 此外，在针的下游侧没有流路切换装置将样品注入到分析流路中，消除了管道与流路切换装置的连接。 这样可以避免样品残留在连接部分，从而减少样品转移并提高分析的准确性。

公开(公告)号：US08496819B2

公开(公告)日：2013-07-30

申请号：US12146912

申请日：2008-06-26

Applicant: Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

Inventor： Katsutoshi Shimizu ,  Masahito Ito ,  Kimihiko Ishii ,  Yoshihiro Nagaoka ,  Kisaburo Deguchi ,  Takefumi Yokokura

IPC: B01D15/08 ,  G01N30/02 ,  C02F1/28

CPC classification number: B01D15/22 ,  B01J20/282 ,  B01J2220/82 ,  B01J2220/825 ,  G01N30/22 ,  G01N30/6047 ,  G01N30/6052 ,  G01N30/6069 ,  G01N2030/522 ,  G01N2030/528

Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using the column are provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

Abstract translation: 提供了具有高耐压性并且即使以小流量进行高速分离和分析的柱和使用该柱的液相色谱装置。 根据本发明的分离柱具有中心的整体式杆，由多孔材料制成，并且具有圆形横截面，填充层布置成使其围绕整体式杆的圆周表面，并且 圆柱形支撑介质布置在填充层外部。 通过在颗粒或珠粒之间填充整体棒和支撑介质之间的管状间隙来形成填充层。

公开(公告)号：US20120216632A1

公开(公告)日：2012-08-30

申请号：US13503694

申请日：2010-10-20

Applicant: Yoshiaki Seki ,  Kimihiko Ishii ,  Mitsuhiko Ueda ,  Katsutoshi Shimizu ,  Hiroshi Suzuki

Inventor： Yoshiaki Seki ,  Kimihiko Ishii ,  Mitsuhiko Ueda ,  Katsutoshi Shimizu ,  Hiroshi Suzuki

IPC: G01N1/22

CPC classification number: G01N30/16 ,  G01N30/20 ,  G01N30/24 ,  G01N2030/207

Abstract: A liquid sample introducing apparatus capable of executing a process with high reliability and high speed is realized. An injection valve includes a sample storage loop connected to a mobile phase flow passage for supplying a sample to a detector of a liquid chromatograph apparatus. A sample is sucked in a needle through a valve and a pipe by a syringe, being introduced to the sample storage loop, being supplied to the mobile phase flow passage. A washing solution from a washing solution bottle is supplied to the needle by a washing unit through the valve and the pipe to wash the inner wall of the needle. The washing solution from the washing solution bottle is supplied to a washing tank by the washing unit through the valve and a pipe to wash the external wall of the needle in the washing tank.

Abstract translation: 实现能够执行高可靠性和高速度的处理的液体样品引入装置。 注射阀包括连接到流动相流动通道的样本储存环，用于将样品供应到液相色谱仪的检测器。 将样品通过注射器通过阀和管吸入针中，并被引入到样品储存环中，供给流动相流动通道。 来自洗涤溶液瓶的洗涤液通过洗涤单元通过阀和管道供应到针中以洗涤针的内壁。 来自洗涤溶液瓶的洗涤溶液通过洗涤单元通过阀门和洗涤槽中的针的外壁来洗涤的洗涤槽。

公开(公告)号：US08191404B2

公开(公告)日：2012-06-05

申请号：US12235145

申请日：2008-09-22

Applicant: Yumiko Ono ,  Kimihiko Ishii ,  Eri Yamashita

Inventor： Yumiko Ono ,  Kimihiko Ishii ,  Eri Yamashita

IPC: G01N13/00

CPC classification number: G01N30/24 ,  G01N30/34 ,  G01N35/1097

Abstract: Disclosed herein is a sample introducing apparatus which is designed such that the analytical flow path runs from the needle to the separation column without the flow path switching means placed at the downstream side of the needle. This design reduces dead volume, which in turn reduces the diffusion of the sample injected into the analytical flow path. Moreover, the absence of the flow path switching means at the downstream side of the needle to inject a sample into the analytical flow path eliminates connection of the pipe with the flow path switching means. This prevents the sample from remaining in the connecting part, thereby reducing sample carry-over and improving the accuracy of analysis.

Abstract translation: 本文公开了一种样品引入装置，其被设计成使得分析流路从针头延伸到分离柱，而流路切换装置设置在针的下游侧。 该设计减少了死体积，这进而降低了注入分析流路的样品的扩散。 此外，在针的下游侧没有流路切换装置将样品注入到分析流路中，消除了管道与流路切换装置的连接。 这样可以避免样品残留在连接部分，从而减少样品转移并提高分析的准确性。