公开(公告)号：US08916746B2

公开(公告)日：2014-12-23

申请号：US12915547

申请日：2010-10-29

申请人： Masakazu Kashihara ,  Toshiyuki Komori ,  Ichiro Oka ,  Satoru Usami ,  Norio Kato ,  Yukoh Hiei ,  Yoshimitsu Takakura ,  Toshihiko Komari ,  Teruyuki Imayama ,  Scott V. Tingey ,  Hajime Sakai ,  Marc C. Albertsen ,  Stanley Luck

发明人： Masakazu Kashihara ,  Toshiyuki Komori ,  Ichiro Oka ,  Satoru Usami ,  Norio Kato ,  Yukoh Hiei ,  Yoshimitsu Takakura ,  Toshihiko Komari ,  Teruyuki Imayama ,  Scott V. Tingey ,  Hajime Sakai ,  Marc C. Albertsen ,  Stanley Luck

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/82

CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N15/821

摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

摘要翻译： 用于赋予耐旱性的分离的多核苷酸和多肽和重组DNA构建体，包含这些重组DNA构建体的组合物（例如植物或种子），以及利用这些重组DNA构建体的方法。 重组DNA构建体包含可操作地连接于在植物中有功能的启动子的多核苷酸，其中所述多核苷酸编码DTP21多肽。

公开(公告)号：US20100132068A1

公开(公告)日：2010-05-27

申请号：US12306163

申请日：2007-06-25

申请人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

发明人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

IPC分类号： A01H1/00 ,  C12N15/63

CPC分类号： C12N15/82 ,  C12N15/8205

摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.

摘要翻译： 本发明旨在提供用于植物转化的新型载体。 本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。

公开(公告)号：US08298819B2

公开(公告)日：2012-10-30

申请号：US12306163

申请日：2007-06-25

申请人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

发明人： Yoshimitsu Takakura ,  Toshihiko Komari ,  Yuji Ishida ,  Toshiyuki Komori ,  Yukoh Hiei ,  Toshiki Mine ,  Teruyuki Imayama

IPC分类号： C12N15/82 ,  C12N15/19 ,  C12N15/00

CPC分类号： C12N15/82 ,  C12N15/8205

摘要： The present invention aims to provide novel vectors for plant transformation.The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E. coli and a bacterium of the genus Agrobacterium; 7) they contain a T-DNA right border sequence of a bacterium of the genus Agrobacterium; 8) they contain a T-DNA left border sequence of a bacterium of the genus Agrobacterium; 9) they contain a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) they contain restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.

摘要翻译： 本发明旨在提供用于植物转化的新型载体。 本发明的载体是全长为15kb或更小的粘粒载体，其特征在于：1）它们含有IncP质粒的复制起点，但不含有其他质粒组的任何复制起点; 2）它们含有IncP质粒的trfA1基因; 3）它们含有一个IncP质粒的oriT; 4）它们含有IncP质粒的incC1基因; 5）它们含有λ噬菌体的cos位点，cos位点位于T-DNA外部; 6）它们含有在大肠杆菌中表达的耐药基因和农杆菌属的细菌; 7）它们含有农杆菌属细菌的T-DNA右侧序列; 8）它们含有农杆菌属细菌的T-DNA左边界序列; 9）它们含有位于7）和8之间的植物转化的选择性标记基因并在植物中表达; 和10）它们含有位于7）和8之间的限制性内切核酸酶识别位点，用于克隆外来基因。

公开(公告)号：US20080301832A1

公开(公告)日：2008-12-04

申请号：US10576693

申请日：2004-10-22

申请人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

发明人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

IPC分类号： C12N15/11 ,  C12Q1/68 ,  A01H5/00 ,  C12P19/28

CPC分类号： A01H1/00 ,  A01H1/04 ,  C12N15/8201 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement.The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

摘要翻译： 本发明提供了一种选择可用于提供具有农业上有利改进的植物的基因组DNA片段的方法。 本发明的方法包括以下步骤：1）从植物制备基因组DNA，然后将其克隆到克隆载体中以形成基因组DNA文库; 2）将构成基因组DNA文库的每个基因组克隆的基因组片段分别引入植物中以产生转基因植物; 3）培育转基因植物或其后代以选择表现出农业上有利的表型变异的植物; 和4）将在步骤（2）中引入的基因组DNA片段选择在步骤（3）中选择的植物中，作为目的基因组DNA片段。

公开(公告)号：US08039687B2

公开(公告)日：2011-10-18

申请号：US10576693

申请日：2004-10-22

申请人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

发明人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/29 ,  C12N15/55 ,  C12N15/70 ,  C12N15/84

CPC分类号： A01H1/00 ,  A01H1/04 ,  C12N15/8201 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

摘要翻译： 本发明提供了一种选择可用于提供具有农业上有利改进的植物的基因组DNA片段的方法。 本发明的方法包括以下步骤：1）从植物制备基因组DNA，然后将其克隆到克隆载体中以形成基因组DNA文库; 2）将构成基因组DNA文库的每个基因组克隆的基因组片段分别引入植物中以产生转基因植物; 3）培育转基因植物或其后代以选择表现出农业上有利的表型变异的植物; 和4）将在步骤（2）中引入的基因组DNA片段选择在步骤（3）中选择的植物中，作为目的基因组DNA片段。

公开(公告)号：US20060179517A1

公开(公告)日：2006-08-10

申请号：US10520350

申请日：2003-03-17

申请人： Toshiyuki Komori ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Shoichi Suzuki ,  Yoshiki Kuraya

发明人： Toshiyuki Komori ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Shoichi Suzuki ,  Yoshiki Kuraya

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00

CPC分类号： C07K14/415 ,  C12N15/8287 ,  C12N15/8289

摘要： The purpose of the present invention is to provide the rice restorer gene to the rice BT type cytoplasmic male sterility. The gene of the present invention comprises a nucleic acid encoding the amino acid sequence of SEQ ID NO.75, or an amino acid sequence which is identical to at least 70% of the amino acid sequence of SEQ ID NO.75, and which functions to restore fertility. Preferably, the gene of the present invention has the base sequence of SEQ ID NOS:69-74, 80-85 or the bases 43907-46279 of SEQ ID NO:27.

摘要翻译： 本发明的目的是提供水稻恢复基因到水稻BT型细胞质雄性不育。 本发明的基因包含编码SEQ ID NO.75的氨基酸序列的核酸或与SEQ ID NO.75的氨基酸序列的至少70％相同的氨基酸序列，其功能 恢复生育率。 优选地，本发明的基因具有SEQ ID NO：69-74,80-85的碱基序列或SEQ ID NO：27的碱基43907-46279。

公开(公告)号：US07544856B2

公开(公告)日：2009-06-09

申请号：US10520350

申请日：2003-03-17

申请人： Toshiyuki Komori ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Shoichi Suzuki ,  Yoshiki Kuraya

发明人： Toshiyuki Komori ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Shoichi Suzuki ,  Yoshiki Kuraya

IPC分类号： C12N15/82 ,  C12N15/12 ,  C07H21/04

CPC分类号： C07K14/415 ,  C12N15/8287 ,  C12N15/8289

摘要： The purpose of the present invention is to provide the rice restorer gene to the rice BT type cytoplasmic male sterility. The gene of the present invention comprises a nucleic acid encoding the amino acid sequence of SEQ ID NO. 75, or an amino acid sequence which is identical to at least 70% of the amino acid sequence of SEQ ID NO. 75, and which functions to restore fertility. Preferably, the gene of the present invention has the base sequence of SEQ ID NOS:69-74, 80-85 or the bases 43907-46279 of SEQ ID NO:27.

摘要翻译： 本发明的目的是提供水稻恢复基因到水稻BT型细胞质雄性不育。 本发明的基因包含编码SEQ ID NO：1的氨基酸序列的核酸。 75，或与SEQ ID NO：1的氨基酸序列的至少70％相同的氨基酸序列。 75，哪些功能恢复生育。 优选地，本发明的基因具有SEQ ID NO：69-74,80-85的碱基序列或SEQ ID NO：27的碱基43907-46279。

公开(公告)号：US5731179A

公开(公告)日：1998-03-24

申请号：US500952

申请日：1995-08-08

申请人： Toshihiko Komari ,  Yasuhito Saito ,  Yukoh Hiei

发明人： Toshihiko Komari ,  Yasuhito Saito ,  Yukoh Hiei

IPC分类号： C12N15/74 ,  C12N15/82 ,  C12N15/84 ,  C12N15/05 ,  A01H5/00 ,  C12N1/20

CPC分类号： C12N15/743 ,  C12N15/8205

摘要： The invention provides a method for transforming a plant through a bacterium belonging to genus Agrobacterium, comprising transforming plant cells simultaneously with a first T-DNA (1) and a second T-DNA (2); and selecting the cells which acquired drug resistance; the first T-DNA (1) containing a gene giving the drug resistance, which functions in the plant; the second T-DNA (2) containing a desired DNA fragment to be introduced into the plant, the second T-DNA (2) being contained in a hybrid vector; the hybrid vector being prepared by homologous recombination between an acceptor vector and an intermediate vector in the bacterium belonging to genus Agrobacterium; the acceptor vector containing at least (a) a DNA region having a function to replicate a plasmid in the bacterium belonging to genus Agrobacterium and Escherichia coli, (b) a DNA region containing virB gene and virG gene in virulence region of Ti plasmid pTiBo542 of Agrobacterium tumefaciens, and (c) a DNA region which is homologous with a part of the intermediate vector, which is subjected to homologous recombination in the bacterium belonging to genus Agrobacterium; the intermediate vector containing at least (i) a DNA region having a function to replicate a plasmid in Escherichia coli, which does not function in the bacterium belonging to genus Agrobacterium, (ii) a DNA region which is homologous with a part of the acceptor vector, which is subjected to homologous recombination in the bacterium belonging to genus Agrobacterium, and (iii) a DNA region which constitutes at least a part of the second T-DNA.

摘要翻译： PCT No.PCT / JP94 / 02049 Sec。 371日期：1995年8月8日 102（e）日期1995年8月8日PCT 1994年12月6日PCT PCT。 公开号WO95 / 16031 日期：1995年6月15日本发明提供了通过农杆菌属细菌转化植物的方法，其包括与第一T-DNA（1）和第二T-DNA（2）同时转化植物细胞; 并选择获得耐药性的细胞; 第一个含有赋予药物抗性的基因的T-DNA（1）在植物中起作用; 第二T-DNA（2）含有待引入植物的所需DNA片段，第二T-DNA（2）包含在杂交载体中; 杂交载体通过在土壤杆菌属的细菌中的受体载体和中间载体之间的同源重组而制备; 所述受体载体至少含有（a）具有复制属于农杆菌属和大肠杆菌属的细菌中的质粒的功能的DNA区域，（b）含有TiBp质粒pTiBo542的毒力区域中的virB基因和virG基因的DNA区域 根癌农杆菌，和（c）与属于农杆菌属细菌的同源重组的中间载体的一部分同源的DNA区域; 所述中间载体至少含有（i）具有在大肠杆菌中复制质粒的功能的DNA区域，其在属于农杆菌属的细菌中不起作用，（ii）与受体的一部分同源的DNA区域 载体，其在属于农杆菌属的细菌中进行同源重组，和（iii）构成第二T-DNA的至少一部分的DNA区。

公开(公告)号：US07939328B1

公开(公告)日：2011-05-10

申请号：US08428238

申请日：1994-09-01

申请人： Hideaki Saito ,  Yuji Ishida ,  Yukoh Hiei ,  Toshihiko Komari

发明人： Hideaki Saito ,  Yuji Ishida ,  Yukoh Hiei ,  Toshihiko Komari

IPC分类号： C12N15/82

CPC分类号： C12N15/8205

摘要： Disclosed is a method of transforming monocotyledons which necessitates only a short period from the transformation to the regeneration of a whole plant as compared with the conventional methods, thus reducing the frequency of occurrence of mutants, and can be generally applied to the plants for which any system of regenerating the whole plants from protoplasts has not been established, and in which the material to be used can be readily prepared without any particular apparatuses. The present invention provides a method for transforming monocotyledons comprising transforming scutellum of an immature embryo of a monocotyledon with a bacterium belonging to genus Agrobacterium containing a desired gene, which immature embryo has not been subjected to a dedifferentiation treatment, to obtain a transformant.

摘要翻译： 本发明公开了一种转化单子叶植物的方法，其与常规方法相比仅需要从整个植物的转化到再生的短的时间，从而降低突变体的发生频率，并且通常可以应用于任何 尚未建立从原生质体再生整株植物的系统，其中待使用的材料可以在没有任何特定装置的情况下容易地制备。 本发明提供一种用于将单子叶植物的未成熟胚的鳞片状细菌与含有未成熟胚的未分化处理的所需基因的农杆菌属的农杆菌属转化成单转基因的方法，得到转化体。

公开(公告)号：US07060876B2

公开(公告)日：2006-06-13

申请号：US09229324

申请日：1999-01-13

申请人： Yukoh Hiei ,  Toshihiko Komari

发明人： Yukoh Hiei ,  Toshihiko Komari

IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H2/04 ,  C12N15/82 ,  C12N15/84

CPC分类号： C12N15/8205

摘要： The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods. The inventive method may be generally applied even to the plants for which a regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledonous plant, comprising contacting a cultured tissue of said monocotyledonous plant during dedifferentiation thereof obtained by culturing an explant on a dedifferentiation-inducing medium for less than 7 days with a bacterium belonging to the genus Agrobacterium containing a super binary vector having the virulence region of a Ti plasmid, left and right border sequences of T-DNA of a Ti plasmid or an Ri plasmid of a bacterium belonging to the genus Agrobacterium, and a desired gene located between said left and right border sequences.

摘要翻译： 本发明涉及一种用于转化单子叶植物的方法。 使用本发明的方法从植物的转化到再生所需的时间更短，使得突变体的出现频率比常规方法小。 本发明的方法通常可以应用于从原生质体到植物的再生方法尚未建立的植物，并且通过该方法对待处理的材料的制备是容易的。 也就是说，本发明提供了一种用于转化单子叶植物的方法，包括使所述单子叶植物的去分化过程中的培养的组织与通过在去分化诱导培养基上培养少于7天的外植体与属于该属的细菌接触 含有具有Ti质粒的毒力区域的超级二元载体的农杆菌，Ti质粒的T-DNA的左侧和右侧边界序列或属于农杆菌属的细菌的Ri质粒，以及位于所述左侧和 右边界序列