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1.发明授权Integral multi-layered element containing glucose oxidase for determining glucose 失效含葡萄糖氧化酶的整体多层元素用于测定葡萄糖
公开(公告)号：US4452887A
公开(公告)日：1984-06-05
申请号：US389344
申请日：1982-06-17
申请人： Masao Kitajima , Fuminori Arai , Harumi Katsuyama
发明人： Masao Kitajima , Fuminori Arai , Harumi Katsuyama
CPC分类号： C12Q1/54 , G01N33/526 , Y10S435/805
摘要： In a dry-type multilayer liquid analysis material for glucose determination composed of a transparent water-impermeable support having formed thereon in sequence at least a reagent layer containing a reactive component forming a detectable material by the action of hydrogen peroxide, a non-porous light-shielding layer, and a porous spreading layer, the coloring efficiency for colorimetry using the liquid analysis material is improved by incorporating a glucose oxidase enzyme in at least one layer disposed above the reagent layer.
摘要翻译： 在用于葡萄糖测定的干式多层液体分析材料中，其由透明的不透水支持体组成，其中至少在其上形成有通过过氧化氢的作用形成可检测材料的反应性成分的试剂层，非多孔光 并且多孔扩散层，通过在设置在试剂层上方的至少一层中并入葡萄糖氧化酶来提高使用液体分析材料的比色法的着色效率。
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公开(公告)号：US4781890A
公开(公告)日：1988-11-01
申请号：US11386
申请日：1987-02-05
CPC分类号： C12Q1/26 , G01N31/22 , G01N33/525 , Y10S435/805 , Y10T436/144444 , Y10T436/25125
摘要： A multilayer chemical analytical element is employable in quantitative analysis of analyte in a biological fluid such as blood. The element consists of a porous spreading layer, a light-blocking layer and a reagent layer on a water-impermeable light-transmissive support in order. The light-blocking layer contains a titanium dioxide fine powder with no provision of aluminum oxide compound or silicon oxide.
摘要翻译： 多层化学分析元件可用于定量分析生物流体如血液中的分析物。 该元件按顺序由不透水的透光支架上的多孔扩散层，遮光层和试剂层组成。 遮光层含有不含氧化铝化合物或氧化硅的二氧化钛细粉末。
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公开(公告)号：US4604347A
公开(公告)日：1986-08-05
申请号：US577090
申请日：1984-02-06
CPC分类号： G01N33/525 , G01N31/221 , Y10S435/805
摘要： An analytical element containing at least one reagent for dry analysis of analyte in a liquid sample, which contains a nondiffusive acid and a nondiffusive base in different phases, being capable of adjusting the surrounding pH of the reaction area of at least one reagent under fluid contact therebetween, and said at least one reagent is contained in said nondiffusive acid-containing phase or nondiffusive base-containing phase.
摘要翻译： 包含至少一种用于干燥分析液体样品中的分析物的分析元件，其包含不同相中的非扩散性酸和非扩散性碱，能够调节流体接触下至少一种试剂的反应区域的周围pH值 并且所述至少一种试剂包含在所述非扩散性含酸相或非扩散性含碱相中。
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公开(公告)号：USH930H
公开(公告)日：1991-06-04
申请号：US321977
申请日：1989-03-10
申请人： Keiko Kato , Fuminori Arai , Harumi Katsuyama
发明人： Keiko Kato , Fuminori Arai , Harumi Katsuyama
CPC分类号： C12Q1/54
摘要： A dry-type quantitative glucose-analyzing element comprising a water-impermeable light-transmissive support having thereon a group of water-permeable layers comprising a reagent layer, an enzyme-containing layer, and a porous spreading layer which are laminated on said support in this order, and a hydrogen peroxide-detecting coloring composition comprising a hydrogen donor and a coupler; whereinsaid reagent layer contains at least said coupler;said enzyme-containing layer contains a glucose oxidase, a peroxidase and a mordant which immobilizes a dye formed by said coloring composition; andsaid hydrogen donor is contained in at least one water-permeable layer of said group. Using the element, quantitative determination of glucose of high concentration is possible with high accuracy.
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公开(公告)号：US4671937A
公开(公告)日：1987-06-09
申请号：US626743
申请日：1984-07-02
CPC分类号： C12Q1/40 , G01N33/525 , Y10S435/805 , Y10S436/81 , Y10S436/903 , Y10S436/904 , Y10S436/91
摘要： A multilayer analytical element for analysis of analyte in a liquid sample comprising a liquid sample-spreading layer, a reagent layer containing a diazonium salt, and a liquid-impermeable light-transmissive support, being laminated in this order, in which said reagent layer contains a sulfonic acid group-containing polymer having at least 10 molar % of a repeating unit selected from those consisting of a sulfoalkyl group and sulfophenyl group. The analytical element can be an element comprising a porous reagent layer containing a diazonium salt and the liquid-impermeable light-transmissive support, being laminated in this order.
摘要翻译： 用于分析液体样品中的分析物的多层分析元件，其包含液体样品扩散层，含有重氮盐的试剂层和不透液体的透光性载体，其中所述试剂层含有 具有至少10摩尔％的选自由磺基烷基和磺基苯基组成的重复单元的含磺酸基的聚合物。 分析元件可以是包含含有重氮盐的多孔试剂层和液体不可透过的透光性支撑体的元件，其顺序层叠。
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公开(公告)号：US5183741A
公开(公告)日：1993-02-02
申请号：US603243
申请日：1990-10-22
申请人： Fuminori Arai , Harumi Katsuyama
发明人： Fuminori Arai , Harumi Katsuyama
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , Y10S435/805
摘要： An integral multilayer element for chemical analysis utilizing an oxidase enzyme reaction system is prepared having in the following order:(a) a porous spreading layer;(b) an oxygen-permeable, protein-impermeable light-blocking layer;(c) an oxidase-containing layer;(d) an indicator layer containing peroxidase and a hydrogen peroxide indicator showing detectable change in the presence of peroxidase and hydrogen peroxide; and(e) a water-impermeable, light-transmissive support. The oxidase in the oxidase-containing layer is preferably glucose oxidase to provide a multilayer element for glucose determination. This multilayer element shortens the analytical time, widens the measurable concentration range of the analyte and improves accuracy.
摘要翻译： 按照以下顺序制备用于利用氧化酶反应体系的化学分析的整体多层元件：（a）多孔扩散层; （b）透氧的蛋白质不透过的遮光层; （c）含氧化酶层; （d）包含过氧化物酶和过氧化氢指示剂的指示剂层，显示过氧化物酶和过氧化氢存在下可检测的变化; 和（e）不透水的透光支架。 氧化酶层中的氧化酶优选为葡萄糖氧化酶，以提供用于葡萄糖测定的多层元件。 该多层元件缩短了分析时间，扩大了分析物的可测量浓度范围，提高了精度。
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7.发明授权Analytical element having a polyacrylamide or derivative to minimize background for assaying enzyme activity 失效具有聚丙烯酰胺或衍生物的分析元件以最小化测定酶活性的背景
公开(公告)号：US5006458A
公开(公告)日：1991-04-09
申请号：US135670
申请日：1987-12-21
CPC分类号： G01N33/525 , Y10S435/805 , Y10S436/81 , Y10S436/904
摘要： A dry-type analytical element suitable for measuring enzyme activity of in a liquid sample, characterized by incorporating a polyacrylamide, polymethacrylamide or their derivatives into at least one water-permeable layer. The background concentration of the dry-type analytical element exhibits minimal increase even under a fluorescent light, and allows an accurate measured value to be easily obtained.
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公开(公告)号：US4786595A
公开(公告)日：1988-11-22
申请号：US858545
申请日：1986-04-29
CPC分类号： C12Q1/26 , C12Q1/40 , C12Q1/60 , G01N31/22 , G01N33/525 , Y10S436/805
摘要： An analytical element for quantitative analysis of analyte contained in a body fluid or the like, which has at least one reagent layer comprising a reactive reagent and a binder, characterized in that a portion or whole of the binder is a polycarboxylic acid carrying a nonionic surface active agent attached through ester linkage to at least a portion of the carboxyl groups contained therein.
摘要翻译： 用于定量分析包含在体液等中的分析物的分析元件，其具有至少一个包含反应性试剂和粘合剂的试剂层，其特征在于，所述粘合剂的一部分或全部是携带非离子表面的多元羧酸 活性剂通过酯键连接至其中所含羧基的至少一部分。
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公开(公告)号：US5066462A
公开(公告)日：1991-11-19
申请号：US401494
申请日：1989-08-29
CPC分类号： C12Q1/48 , G01N33/525 , Y10S435/805
摘要： A dry-type analytical element suitable for measuring the activity of alanine aminotransferase in a liquid sample, characterized by incorporating a dye capable of absorbing the electromagnetic waves of 400 to 500 nm into at least one water-permeable layer. The coloring sensitivity of the dry-type analytical element does not increase substantially even under a fluorescent light, and thereby, an accurate measured value can easily be obtained.
摘要翻译： 适用于测量液体样品中丙氨酸氨基转移酶活性的干型分析元件，其特征在于将能够吸收400-500nm电磁波的染料掺入到至少一个透水层中。 即使在荧光下，干式分析元件的着色灵敏度也不会显着增加，从而可以容易地获得准确的测量值。
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10.发明授权
公开(公告)号：US4900665A
公开(公告)日：1990-02-13
申请号：US185294
申请日：1988-04-19
CPC分类号： G01N33/526 , C12Q1/42 , C12Q2334/10 , Y10S435/805
摘要： An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an alkaline phosphatase-sensitive self-developing substrate, a buffer layer containing one or more compounds functioning as a phosphoric acid acceptor and a buffering agent and a support layer in a laminated form.
摘要翻译： 一种用于测量碱性磷酸酶活性的整体多层分析元件，其包含含有碱性磷酸酶敏感的自显影底物的机织织物或编织物的多孔扩散层，含有一种或多种用作磷酸盐的化合物的缓冲层 酸受体和缓冲剂和层叠形式的支撑层。
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