公开(公告)号：US20090061485A1

公开(公告)日：2009-03-05

申请号：US11793712

申请日：2004-12-22

申请人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Yasuo Sekimori ,  Kiyoshi Habu ,  Masamichi Sugimoto

发明人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Yasuo Sekimori ,  Kiyoshi Habu ,  Masamichi Sugimoto

IPC分类号： C12P21/08 ,  C12N5/06 ,  C12N5/10 ,  C12P21/02

CPC分类号： C12N9/1081 ,  A01K2217/075 ,  A01K2227/10 ,  C07K16/00 ,  C07K2317/41

摘要： The present invention relates to a method of producing a recombinant protein, particularly an antibody, using a cell in which the function of a fucose transporter is inhibited, and it also provides a cell in which the expression of fucose transporter genes on both homologous chromosomes is artificially suppressed.

摘要翻译： 本发明涉及使用其中岩藻糖转运蛋白的功能被抑制的细胞产生重组蛋白质，特别是抗体的方法，并且还提供了在两个同源染色体上表达岩藻糖转运蛋白基因的细胞是 人为压制

公开(公告)号：US07863042B2

公开(公告)日：2011-01-04

申请号：US10561191

申请日：2004-06-18

申请人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Yasuo Sekimori ,  Kenju Ueno ,  Kiyoshi Habu

发明人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Yasuo Sekimori ,  Kenju Ueno ,  Kiyoshi Habu

IPC分类号： C07H17/00

CPC分类号： C07K14/47 ,  C12P21/005 ,  G01N2500/00

摘要： The present invention provides a gene encoding a fucose transporter, a fucose transporter polypeptide, a method for screening for a compound that binds to a fucose transporter or a compound that inhibits fucose transport activity, a cell having inhibited fucose transporter functions, and a cell wherein the expression of the fucose transporter is inhibited. The present invention further relates to a method for producing recombinant protein, and specifically, to a method for producing protein by which fucose existing within the Golgi apparatus of a host cell is decreased, a method for inhibiting the addition of fucose to protein by which fucose existing within the Golgi apparatus of a host cell is decreased upon production of recombinant protein using the host cell, a method for increasing the cytotoxic activity of an antibody by which an antibody is produced using a cell wherein fucose existing within the Golgi apparatus is decreased, and a cell having a Golgi apparatus wherein fucose existing within the Golgi apparatus is decreased.

摘要翻译： 本发明提供了编码岩藻糖转运蛋白，岩藻糖转运蛋白多肽，筛选结合岩藻糖转运蛋白的化合物或抑制岩藻糖转运活性的化合物的方法，具有抑制岩藻糖转运蛋白功能的细胞的基因，以及细胞，其中 岩藻糖转运蛋白的表达被抑制。 本发明还涉及一种生产重组蛋白的方法，具体地说涉及一种产生蛋白质的方法，通过该方法，存在于宿主细胞高尔基体内的岩藻糖减少，一种抑制岩藻糖加入岩藻糖蛋白质的方法 在使用宿主细胞产生重组蛋白质时，存在于宿主细胞的高尔基体内的存在的方法，使用其中存在于高尔基体中的岩藻糖减少的细胞增加抗体产生抗体的方法， 和具有高尔基体的细胞，其中存在于高尔基体内的岩藻糖减少。

公开(公告)号：US20060246456A1

公开(公告)日：2006-11-02

申请号：US10561191

申请日：2004-06-18

申请人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Yasuo Sekimori ,  Kenju Ueno ,  Kiyoshi Habu

发明人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Yasuo Sekimori ,  Kenju Ueno ,  Kiyoshi Habu

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C07K16/28 ,  C07K14/705

CPC分类号： C07K14/47 ,  C12P21/005 ,  G01N2500/00

摘要： The present invention provides a gene encoding a fucose transporter, a fucose transporter polypeptide, a method for screening for a compound that binds to a fucose transporter or a compound that inhibits fucose transport activity, a cell having inhibited fucose transporter functions, and a cell wherein the expression of the fucose transporter is inhibited. The present invention further relates to a method for producing recombinant protein, and specifically, to a method for producing protein by which fucose existing within the Golgi apparatus of a host cell is decreased, a method for inhibiting the addition of fucose to protein by which fucose existing within the Golgi apparatus of a host cell is decreased upon production of recombinant protein using the host cell, a method for increasing the cytotoxic activity of an antibody by which an antibody is produced using a cell wherein fucose existing within the Golgi apparatus is decreased, and a cell having a Golgi apparatus wherein fucose existing within the Golgi apparatus is decreased.

摘要翻译： 本发明提供了编码岩藻糖转运蛋白，岩藻糖转运蛋白多肽，筛选结合岩藻糖转运蛋白的化合物或抑制岩藻糖转运活性的化合物的方法，具有抑制岩藻糖转运蛋白功能的细胞的基因，以及细胞，其中 岩藻糖转运蛋白的表达被抑制。 本发明还涉及一种生产重组蛋白的方法，具体地说涉及一种生物蛋白质的制造方法，其中存在于宿主细胞高尔基体内的岩藻糖减少，一种抑制岩藻糖加入蛋白质的方法，通过该方法将岩藻糖 在使用宿主细胞产生重组蛋白质时，存在于宿主细胞的高尔基体内的存在的方法，使用其中存在于高尔基体中的岩藻糖减少的细胞增加抗体产生抗体的方法， 和具有高尔基体的细胞，其中存在于高尔基体内的岩藻糖减少。

公开(公告)号：US20070142627A1

公开(公告)日：2007-06-21

申请号：US10567856

申请日：2004-08-11

申请人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Masamichi Sugimoto

发明人： Masayuki Tsuchiya ,  Shigeyuki Iijima ,  Izumi Sugo ,  Masamichi Sugimoto

IPC分类号： C12P21/06 ,  C07K16/46

CPC分类号： C07K16/3061 ,  C07K2317/41 ,  C07K2317/72 ,  C07K2317/732

摘要： An antibody (anti-HM1.24 antibody) against HM1.24 antigen in which the alteration of sugar chains resulted in enhanced antibody-dependent cellular cytotoxicity (ADCC), specifically an antibody having a sugar chain that does not contain α-1,6 core fucose, an antibody having a sugar chain that contains a bisecting N-acetylglucosamine (GlcNAc) structure, or an antibody having a sugar chain that does not contain α-1,6 core fucose and having a sugar chain that contains a bisecting N-acetylglucosamine (GlcNAc) structure.

摘要翻译： 针对HM1.24抗原的抗体（抗HM1.24抗体），其中糖链的改变导致增强的抗体依赖性细胞毒性（ADCC），特别是具有不含α-1,6的糖链的抗体 核糖体岩藻糖，具有含有二等分N-乙酰葡糖胺（GlcNAc）结构的糖链的抗体或具有不含有α-1,6核心岩藻糖的糖链并具有含有二等分N- 乙酰葡糖胺（GlcNAc）结构。

公开(公告)号：US09102739B2

公开(公告)日：2015-08-11

申请号：US12089957

申请日：2006-10-11

申请人： Gregory Alan Lazar ,  Bassil I. Dahiyat ,  Hisafumi Okabe ,  Masamichi Sugimoto ,  Shigeyuki Iijima ,  Izumi Sugo

发明人： Gregory Alan Lazar ,  Bassil I. Dahiyat ,  Hisafumi Okabe ,  Masamichi Sugimoto ,  Shigeyuki Iijima ,  Izumi Sugo

IPC分类号： C07K16/00 ,  C12P21/08 ,  C12P21/06 ,  A61K39/395 ,  A61K39/40 ,  C07K16/30 ,  C07K1/00 ,  A61K39/00

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/72 ,  C07K2317/732

摘要： An anti-glypican-3 antibody comprising one or more amino acid substitutions introduced in the Fc region is disclosed. Preferably, in the anti-glypican-3 antibody, one or more of the amino acid residues at the positions 239, 298, 326, 330 and 332 in the Fc region are substituted with other amino acid residues. Since the Fc-modified anti-glypican-3 antibody of the invention exhibit enhanced ADCC activity, it is useful in treating cancers, such as hepatic cancer. Also disclosed are an anticancer agent comprising the anti-glypican-3 antibody of the invention and a pharmaceutically acceptable carrier, as well as a method of treating a patient with cancer comprising administering to the patient the anticancer agent of the invention.

摘要翻译： 公开了包含在Fc区中引入的一个或多个氨基酸取代的抗磷脂酰肌醇蛋白聚糖-3抗体。 优选地，在抗磷脂酰肌醇蛋白聚糖-3抗体中，Fc区中位置239,298,326,330和332处的一个或多个氨基酸残基被其它氨基酸残基取代。 由于本发明的Fc修饰的抗磷脂酰肌醇蛋白聚糖-3抗体表现出增强的ADCC活性，因此可用于治疗诸如肝癌的癌症。 还公开了包含本发明的抗磷脂酰肌醇蛋白聚糖-3抗体和药学上可接受的载体的抗癌剂，以及治疗患有癌症的患者的方法，包括向患者施用本发明的抗癌剂。

公开(公告)号：US20080267979A1

公开(公告)日：2008-10-30

申请号：US12089957

申请日：2006-10-11

申请人： Gregory Alan Lazar ,  Bassil I. Dahiyat ,  Hisafumi Okabe ,  Masamichi Sugimoto ,  Shigeyuki Iijima ,  Izumi Sugo

发明人： Gregory Alan Lazar ,  Bassil I. Dahiyat ,  Hisafumi Okabe ,  Masamichi Sugimoto ,  Shigeyuki Iijima ,  Izumi Sugo

IPC分类号： A61K39/395 ,  C12P21/08 ,  C07K16/18 ,  A61P35/00

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/72 ,  C07K2317/732

摘要： An anti-glypican-3 antibody comprising one or more amino acid substitutions introduced in the Fc region is disclosed. Preferably, in the anti-glypican-3 antibody, one or more of the amino acid residues at the positions 239, 298, 326, 330 and 332 in the Fc region are substituted with other amino acid residues. Since the Fc-modified anti-glypican-3 antibody of the invention exhibit enhanced ADCC activity, it is useful in treating cancers, such as hepatic cancer. Also disclosed are an anticancer agent comprising the anti-glypican-3 antibody of the invention and a pharmaceutically acceptable carrier, as well as a method of treating a patient with cancer comprising administering to the patient the anticancer agent of the invention.

摘要翻译： 公开了包含在Fc区中引入的一个或多个氨基酸取代的抗磷脂酰肌醇蛋白聚糖-3抗体。 优选地，在抗磷脂酰肌醇蛋白聚糖-3抗体中，Fc区中位置239,298,326,330和332处的一个或多个氨基酸残基被其它氨基酸残基取代。 由于本发明的Fc修饰的抗磷脂酰肌醇蛋白聚糖-3抗体表现出增强的ADCC活性，因此可用于治疗诸如肝癌的癌症。 还公开了包含本发明的抗磷脂酰肌醇蛋白聚糖-3抗体和药学上可接受的载体的抗癌剂，以及治疗患有癌症的患者的方法，包括向患者施用本发明的抗癌剂。

公开(公告)号：US07867734B2

公开(公告)日：2011-01-11

申请号：US11577944

申请日：2005-10-26

申请人： Kiyotaka Nakano ,  Izumi Sugo ,  Masamichi Sugimoto ,  Takahiro Ishiguro ,  Megumi Tanaka ,  Shigeyuki Iijima

发明人： Kiyotaka Nakano ,  Izumi Sugo ,  Masamichi Sugimoto ,  Takahiro Ishiguro ,  Megumi Tanaka ,  Shigeyuki Iijima

IPC分类号： C12P21/04 ,  C07K16/00

CPC分类号： C07K16/303 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/72 ,  C07K2317/732

摘要： An anti-glypican 3 antibody with modified sugar chains, more specifically, an anti-glypican 3 antibody lacking fucose is provided. The anti-glypican 3 antibody with modified sugar chains of the present invention may be produced by a process comprising introducing a nucleic acid encoding an anti-glypican 3 antibody into host cells with reduced fucose addition capability, such as YB2/0 cells and cells lacking a fucose transporter. The anti-glypican 3 antibody with modified sugar chains of the present invention has a high level of cytotoxic activity and therefore is useful as a cell growth inhibitor such as an anticancer agent.

摘要翻译： 提供了具有修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体，更具体地，提供了缺乏岩藻糖的磷脂酰肌醇蛋白聚糖3抗体。 具有本发明的修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体可以通过包括将编码抗磷脂酰肌醇蛋白聚糖3抗体的核酸引入具有降低的岩藻糖加成能力的宿主细胞中的方法来生产，例如YB2 / 0细胞和缺乏细胞的细胞 岩藻糖转运蛋白 具有本发明的修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体具有高水平的细胞毒活性，因此可用作细胞生长抑制剂如抗癌剂。

公开(公告)号：US20070087005A1

公开(公告)日：2007-04-19

申请号：US11251561

申请日：2005-10-14

申请人： Gregory Lazar ,  Bassil Dahiyat ,  Hisafumi Okabe ,  Masamichi Sugimoto ,  Shigeyuki Iijima ,  Izumi Sugo

发明人： Gregory Lazar ,  Bassil Dahiyat ,  Hisafumi Okabe ,  Masamichi Sugimoto ,  Shigeyuki Iijima ,  Izumi Sugo

IPC分类号： C07K16/30 ,  A61K39/395

CPC分类号： C07K16/18 ,  A61K2039/505 ,  C07K16/303 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/732

摘要： An anti-glypican-3 antibody comprising one or more amino acid substitutions introduced in the Fc region is disclosed. Preferably, in the anti-glypican-3 antibody, one or more of the amino acid residues at the positions 239, 298, 326, 330 and 332 in the Fc region are substituted with other amino acid residues. Since the Fc-modified anti-glypican-3 antibody of the invention exhibit enhanced ADCC activity, it is useful in treating cancers, such as hepatic cancer. Also disclosed are an anticancer agent comprising the anti-glypican-3 antibody of the invention and a pharmaceutically acceptable carrier, as well as a method of treating a patient with cancer comprising administering to the patient the anticancer agent of the invention.

摘要翻译： 公开了包含在Fc区中引入的一个或多个氨基酸取代的抗磷脂酰肌醇蛋白聚糖-3抗体。 优选地，在抗磷脂酰肌醇蛋白聚糖-3抗体中，Fc区中位置239,298,326,330和332处的一个或多个氨基酸残基被其它氨基酸残基取代。 由于本发明的Fc修饰的抗磷脂酰肌醇蛋白聚糖-3抗体表现出增强的ADCC活性，因此可用于治疗诸如肝癌的癌症。 还公开了包含本发明的抗磷脂酰肌醇蛋白聚糖-3抗体和药学上可接受的载体的抗癌剂，以及治疗患有癌症的患者的方法，包括向患者施用本发明的抗癌剂。

公开(公告)号：US20110033452A1

公开(公告)日：2011-02-10

申请号：US12852950

申请日：2010-08-09

申请人： Kiyotaka Nakano ,  Izumi Sugo ,  Masamichi Sugimoto ,  Takahiro Ishiguro ,  Megumi Tanaka ,  Shigeyuki Iijima

发明人： Kiyotaka Nakano ,  Izumi Sugo ,  Masamichi Sugimoto ,  Takahiro Ishiguro ,  Megumi Tanaka ,  Shigeyuki Iijima

IPC分类号： A61K39/395 ,  C07K16/00 ,  C12P21/08 ,  C12N5/071 ,  A61P35/00

CPC分类号： C07K16/303 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/72 ,  C07K2317/732

摘要： An anti-glypican 3 antibody with modified sugar chains, more specifically, an anti-glypican 3 antibody lacking fucose is provided. The anti-glypican 3 antibody with modified sugar chains of the present invention may be produced by a process comprising introducing a nucleic acid encoding an anti-glypican 3 antibody into host cells with reduced fucose addition capability, such as YB2/0 cells and cells lacking a fucose transporter. The anti-glypican 3 antibody with modified sugar chains of the present invention has a high level of cytotoxic activity and therefore is useful as a cell growth inhibitor such as an anticancer agent.

摘要翻译： 提供了具有修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体，更具体地，提供了缺乏岩藻糖的磷脂酰肌醇蛋白聚糖3抗体。 具有本发明的修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体可以通过包括将编码抗磷脂酰肌醇蛋白聚糖3抗体的核酸引入具有降低的岩藻糖加成能力的宿主细胞中的方法来生产，例如YB2 / 0细胞和缺乏细胞的细胞 岩藻糖转运蛋白 具有本发明的修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体具有高水平的细胞毒活性，因此可用作细胞生长抑制剂如抗癌剂。

公开(公告)号：US20080124330A1

公开(公告)日：2008-05-29

申请号：US11577944

申请日：2005-10-26

申请人： Kiyotaka Nakano ,  Izumi Sugo ,  Masamichi Sugimoto ,  Takahiro Ishiguro ,  Megumi Tanaka ,  Shigeyuki Iijima

发明人： Kiyotaka Nakano ,  Izumi Sugo ,  Masamichi Sugimoto ,  Takahiro Ishiguro ,  Megumi Tanaka ,  Shigeyuki Iijima

IPC分类号： A61K39/395 ,  C07K16/18 ,  A61P43/00 ,  C12P21/04

CPC分类号： C07K16/303 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/72 ,  C07K2317/732

摘要： An anti-glypican 3 antibody with modified sugar chains, more specifically, an anti-glypican 3 antibody lacking fucose is provided. The anti-glypican 3 antibody with modified sugar chains of the present invention may be produced by a process comprising introducing a nucleic acid encoding an anti-glypican 3 antibody into host cells with reduced fucose addition capability, such as YB2/0 cells and cells lacking a fucose transporter. The anti-glypican 3 antibody with modified sugar chains of the present invention has a high level of cytotoxic activity and therefore is useful as a cell growth inhibitor such as an anticancer agent.

摘要翻译： 提供了具有修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体，更具体地，提供了缺乏岩藻糖的磷脂酰肌醇蛋白聚糖3抗体。 具有本发明的修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体可以通过包括将编码抗磷脂酰肌醇蛋白聚糖3抗体的核酸引入具有降低的岩藻糖加成能力的宿主细胞中的方法来生产，例如YB2 / 0细胞和缺乏细胞的细胞 岩藻糖转运蛋白 具有本发明的修饰的糖链的磷脂酰肌醇蛋白聚糖3抗体具有高水平的细胞毒活性，因此可用作细胞生长抑制剂如抗癌剂。