公开(公告)号：US12043646B2

公开(公告)日：2024-07-23

申请号：US18068838

申请日：2022-12-20

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Surin Mong

IPC: C07K1/04 ,  C07K7/06 ,  C07K14/47 ,  C07K14/60 ,  C07K14/61 ,  C07K14/62 ,  G01N21/27 ,  G01N21/33 ,  G01N21/3577 ,  G01N21/84 ,  G01N33/487

CPC classification number: C07K1/045 ,  C07K7/06 ,  C07K14/47 ,  C07K14/60 ,  C07K14/61 ,  C07K14/62 ,  G01N21/33 ,  G01N33/48707 ,  G01N21/272 ,  G01N21/3577 ,  G01N2021/8416

Abstract: Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthesis system.

公开(公告)号：US20230279045A1

公开(公告)日：2023-09-07

申请号：US18068838

申请日：2022-12-20

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Surin Mong

IPC: C07K1/04 ,  C07K14/60 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62 ,  G01N21/33 ,  G01N33/487

CPC classification number: C07K1/045 ,  C07K14/60 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62 ,  G01N21/33 ,  G01N33/48707 ,  G01N2021/8416

Abstract: Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthesis system.

公开(公告)号：US20210047365A1

公开(公告)日：2021-02-18

申请号：US16874824

申请日：2020-05-15

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Surin Mong

IPC: C07K1/04 ,  C07K14/60 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62 ,  G01N21/33 ,  G01N33/487

Abstract: Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthesis system.

公开(公告)号：US20170081359A1

公开(公告)日：2017-03-23

申请号：US15267995

申请日：2016-09-16

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Andrea Adamo ,  Patrick Louis Heider ,  Klavs F. Jensen

IPC: C07K1/08 ,  B01J19/24 ,  C07K14/61 ,  C07K7/06 ,  C07K14/62 ,  C07K14/47

CPC classification number: C07K1/084 ,  B01J19/24 ,  B01J2219/24 ,  C07K1/045 ,  C07K7/06 ,  C07K14/47 ,  C07K14/60 ,  C07K14/61 ,  C07K14/62

Abstract: Methods and system for solid phase peptide synthesis are provided. Solid phase peptide synthesis is a known process in which amino acid residues are added to peptides that have been immobilized on a solid support. New amino acid residues are added via a coupling reaction between an activated amino acid and an amino acid residue of the immobilized peptide. Amino acids may be activated using, e.g., a base and an activating agent. Certain inventive concepts, described herein, relate to methods and systems for the activation of amino acids. These systems and methods may allow for fewer side reactions and a higher yield compared to conventional activation techniques as well as the customization of the coupling reaction on a residue-by-residue basis without the need for costly and/or complex processes.

公开(公告)号：US20170081358A1

公开(公告)日：2017-03-23

申请号：US15268032

申请日：2016-09-16

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Surin Mong

IPC: C07K1/04 ,  C07K14/47 ,  C07K14/61 ,  G01N21/33 ,  G01N33/487 ,  G01N21/27 ,  G01N21/3577 ,  C07K7/06 ,  C07K14/62

CPC classification number: C07K1/045 ,  C07K7/06 ,  C07K14/47 ,  C07K14/60 ,  C07K14/61 ,  C07K14/62 ,  G01N21/272 ,  G01N21/33 ,  G01N21/3577 ,  G01N33/48707 ,  G01N2021/8416

Abstract: Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthesis system.

公开(公告)号：US20230174572A1

公开(公告)日：2023-06-08

申请号：US17747952

申请日：2022-05-18

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Andrea Adamo ,  Patrick Louis Heider ,  Klavs F. Jensen

IPC: C07K1/08 ,  C07K1/04 ,  C07K14/60 ,  B01J19/24 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62

CPC classification number: C07K1/084 ,  B01J19/24 ,  C07K1/045 ,  C07K7/06 ,  C07K14/47 ,  C07K14/60 ,  C07K14/61 ,  C07K14/62 ,  B01J2219/24

Abstract: Methods and system for solid phase peptide synthesis are provided. Solid phase peptide synthesis is a known process in which amino acid residues are added to peptides that have been immobilized on a solid support. New amino acid residues are added via a coupling reaction between an activated amino acid and an amino acid residue of the immobilized peptide. Amino acids may be activated using, e.g., a base and an activating agent. Certain inventive concepts, described herein, relate to methods and systems for the activation of amino acids. These systems and methods may allow for fewer side reactions and a higher yield compared to conventional activation techniques as well as the customization of the coupling reaction on a residue-by-residue basis without the need for costly and/or complex processes.

公开(公告)号：US11584776B2

公开(公告)日：2023-02-21

申请号：US16874824

申请日：2020-05-15

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Surin Mong

IPC: C07K1/04 ,  C07K14/60 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62 ,  G01N21/33 ,  G01N33/487 ,  G01N21/84 ,  G01N21/27 ,  G01N21/3577

Abstract: Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthesis system.

公开(公告)号：US20210188901A1

公开(公告)日：2021-06-24

申请号：US17086196

申请日：2020-10-30

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Andrea Adamo ,  Patrick Louis Heider ,  Klavs F. Jensen

IPC: C07K1/08 ,  C07K1/04 ,  C07K14/60 ,  B01J19/24 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62

Abstract: Methods and system for solid phase peptide synthesis are provided. Solid phase peptide synthesis is a known process in which amino acid residues are added to peptides that have been immobilized on a solid support. New amino acid residues are added via a coupling reaction between an activated amino acid and an amino acid residue of the immobilized peptide. Amino acids may be activated using, e.g., a base and an activating agent. Certain inventive concepts, described herein, relate to methods and systems for the activation of amino acids. These systems and methods may allow for fewer side reactions and a higher yield compared to conventional activation techniques as well as the customization of the coupling reaction on a residue-by-residue basis without the need for costly and/or complex processes.

公开(公告)号：US10683325B2

公开(公告)日：2020-06-16

申请号：US15268032

申请日：2016-09-16

Applicant: Massachusetts Institute of Technology

Inventor： Dale Arlington Thomas, III ,  Alexander James Mijalis ,  Bradley L. Pentelute ,  Mark David Simon ,  Surin Mong

IPC: C07K1/04 ,  C07K14/60 ,  C07K7/06 ,  C07K14/47 ,  C07K14/61 ,  C07K14/62 ,  G01N21/33 ,  G01N33/487 ,  G01N21/84 ,  G01N21/27 ,  G01N21/3577

Abstract: Methods and systems for control of solid phase peptide synthesis are generally described. Control of solid phase peptide synthesis involves the use of feedback from one or more reactions and/or processes (e.g., reagent removal) taking place in the solid phase peptide synthesis system. In some embodiments, a detector may detect one or more fluids flowing across a detection zone of a solid phase peptide synthesis system and one or more signals may be generated corresponding to the fluid(s). For instance, an electromagnetic radiation detector positioned downstream of a reactor may detect a fluid exiting the reactor after a deprotection reactor and produce a signal(s). In some embodiments, based at least in part on information derived from the signal(s), a parameter of the system may be modulated prior to and/or during one or more subsequent reactions and/or processes taking place in the solid phase peptide synthesis system. In some embodiments, the methods and systems, described herein, can be used to conduct quality control to determine and correct problems (e.g., aggregation, truncation, deletion) in reactions (e.g., coupling reactions) taking place in the solid phase peptide synthesis system.