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    METHOD OF PROFILING A SAMPLE COMPRISING A PLURALITY OF CELLS AND A SYSTEM FOR PERFORMING THE SAME
    1.
    发明申请
    METHOD OF PROFILING A SAMPLE COMPRISING A PLURALITY OF CELLS AND A SYSTEM FOR PERFORMING THE SAME 有权

    公开(公告)号：US20230039455A1

    公开(公告)日：2023-02-09

    申请号：US17758531

    申请日：2021-01-08

    申请人： Massachusetts Institute of Technology ,  National University Hospital (Singapore) Pte. Ltd.

    发明人： Jongyoon Han ,  Kerwin Zeming Kwek ,  Win Sen Kuan

    IPC分类号： B01L3/00

    摘要： The invention is to provide a method of profiling a sample comprising a plurality of cells, the method comprising: flowing cells from the sample through a first array of pillars to obtain one or more distribution profiles of cells sorted by the first array; flowing cells from the sample through a second array of pillars that is different from the first array of pillars to obtain on one or more distribution profiles of cells sorted by the second array; and deriving a biophysical signature of the sample based on at least the one or more distribution profiles of the cells sorted by the first array and/or the one or more distribution profiles of the cells sorted by the second array. The method further comprises determining a health status of a subject based on the biophysical signature of the sample. The invention is also to provide a sample profiling system. In various embodiments, the distribution profile of cells in the output regions is indicative of one or more biophysical properties of the cells, which may include the size and deformability of the cells. The pillars in the first array and the second array may have a shape selected from the group consisting of a substantially L shape and a substantially inverse L shape, mirror reflections thereof or combinations thereof.

    One-step protein analysis using slanted nanofilter array
    3.
    发明授权
    One-step protein analysis using slanted nanofilter array 有权

    公开(公告)号：US10888863B2

    公开(公告)日：2021-01-12

    申请号：US15512176

    申请日：2015-09-18

    申请人： Massachusetts Institute of Technology

    发明人： Sung Hee Ko ,  Jongyoon Han

    IPC分类号： B01L3/00 ,  G01N27/447 ,  G01N30/00

    摘要： This disclosure provides an apparatus and a method for quickly, efficiently and continuously fractionating biomolecules, such as DNAs and proteins based on size and other factors, while allowing imaging of the separated biomolecules as they are processed within the apparatus. The apparatus employs angled nanochannels to first preconcentrate and then separate like molecules. Its embodiments offer improved detection sensitivity and separation resolution over existing technologies and multiplexing capabilities.

    Multi-Dimensional Double Spiral Device and Methods of Use Thereof
    5.
    发明申请
    Multi-Dimensional Double Spiral Device and Methods of Use Thereof 审中-公开

    公开(公告)号：US20200171488A1

    公开(公告)日：2020-06-04

    申请号：US16683917

    申请日：2019-11-14

    申请人： Massachusetts Institute of Technology

    发明人： Jongyoon Han ,  Hyungkook Jeon

    IPC分类号： B01L3/00

    摘要： Described is a multi-dimensional double spiral (MDDS) microfluidic device comprising a first spiral microchannel and a second microchannel, wherein the wherein the first spiral microchannel and second spiral microchannel have different cross-sectional areas. Also described is a device comprising a multi-dimensional double spiral and system for recirculation. The invention also encompasses methods of separating particles from a sample fluid comprising a mixture of particles comprising the use of the multi-dimensional double spiral microfluidic device.

    Water Desalination/Purification and Bio-Agent Preconcentration by Ion Concentration Polarization
    7.
    发明申请
    Water Desalination/Purification and Bio-Agent Preconcentration by Ion Concentration Polarization 有权
    海水淡化/净化和离子浓度极化的生物剂预浓缩

    公开(公告)号：US20140374274A1

    公开(公告)日：2014-12-25

    申请号：US14306607

    申请日：2014-06-17

    申请人： Massachusetts Institute of Technology

    发明人： Rhokyun Kwak ,  Jongyoon Han

    IPC分类号： C02F1/469

    CPC分类号： C02F1/469 ,  B01D61/42 ,  C02F2103/08 ,  C02F2201/46115

    摘要： Between two juxtaposed similar ion exchange membranes (AEMs or CEMs), an ion depletion zone (dde) and ion enrichment zone (den) are generated under an electric field. As cations are selectively transferred through the CEMs, for example, anions are relocated in order to achieve electro-neutrality, resulting in the concentration drop (increase) in ion depletion (enrichment) zone. The concentration drop (i.e. salt removal) is low and spatially gradual at relatively low voltage or current (i.e. Ohmic regime). However, at higher voltage or current (i.e. overlimiting regime), strong electroconvective vortex accelerates cation transport through CEMs, allowing us to “relocate” most salt ions. The flat depletion zone occurs with significantly low ion concentration, and corresponding strong electric field in the zone, and any charged agents (e.g. proteins and bacteria) cannot penetrate this flat zone. As a result, we can separate and collect the desalted/purified flow from brine flow by bifurcating the channel at the end of the CEMs. This ICP desalination/purification also happens with two anion exchange membranes (AEMs) by relocating cations, but the location of desalted/brine flows are converted.

    摘要翻译： 在两个并置的类似离子交换膜（AEM或CEM）之间，在电场下产生离子耗尽区（dde）和离子富集区（den）。 当阳离子选择性地通过CEM转移时，例如，阴离子被重新定位以实现电中性，导致离子耗尽（富集）区域的浓度下降（增加）。 在相对低的电压或电流（即欧姆方式）下，浓度下降（即去除盐）是低的并且在空间上逐渐变化。 然而，在较高的电压或电流（即覆盖状态）下，强对流涡流加速通过CEMs的阳离子传输，从而允许我们“重新定位”大多数盐离子。 平坦的耗尽区发生在显着低的离子浓度和区域中相应的强电场，并且任何带电剂（例如蛋白质和细菌）不能穿透该平坦区域。 因此，我们可以通过在CEM结束时分流通道来分离和收集来自盐水流的脱盐/净化流。 这种ICP脱盐/纯化也通过重新定位阳离子而发生在两个阴离子交换膜（AEM）上，但是转化了脱盐/盐水流的位置。

    Method for Enhancing Current Throughput in an Electrochemical System
    8.
    发明申请
    Method for Enhancing Current Throughput in an Electrochemical System 有权
    提高电化学系统当前通量的方法

    公开(公告)号：US20140322630A1

    公开(公告)日：2014-10-30

    申请号：US14267336

    申请日：2014-05-01

    申请人： Massachusetts Institute of Technology

    发明人： Sung Hee Ko ,  Sung Jae Kim ,  Jongyoon Han ,  HiongYap Gan

    IPC分类号： G01N27/28 ,  H01M8/02

    CPC分类号： G01N27/28 ,  B01L3/502746 ,  B01L2300/0816 ,  B01L2400/086 ,  H01M8/026 ,  H01M8/1097 ,  H01M2250/00 ,  Y10T29/49108

    摘要： An electrochemical system with reduced limiting-current behavior is disclosed. The electrochemical system is useful for fuel cells and bio-sensors. In part, the invention relates a method of reducing or eliminating limiting-current behavior in the operation electrochemical systems, in particular those with ion-selective membrane or electrochemical electrodes, by spatially reducing the convection near the membrane or the electrode. The invention further relates to electrochemical systems in which micropores, microarrays or pillar arrays are used to reduce convection in comparison to conventional systems without microarrays, micropores or pillar arrays.

    摘要翻译： 公开了一种具有减小的限流特性的电化学系统。 电化学系统可用于燃料电池和生物传感器。 部分地，本发明涉及通过空间上减少膜或电极附近的对流来减少或消除操作电化学系统中的极限电流行为的方法，特别是具有离子选择性膜或电化学电极的那些。 本发明还涉及与没有微阵列，微孔或柱阵列的常规系统相比，其中使用微孔，微阵列或柱阵列来减少对流的电化学系统。