公开(公告)号：US07141429B2

公开(公告)日：2006-11-28

申请号：US10268620

申请日：2002-10-09

申请人： Matthew S. Munson ,  Catherine R. Cabrera ,  Paul Yager ,  Anson Hatch ,  Andrew Kamholz

发明人： Matthew S. Munson ,  Catherine R. Cabrera ,  Paul Yager ,  Anson Hatch ,  Andrew Kamholz

IPC分类号： G01N35/08 ,  B01D17/00

CPC分类号： B01F13/0076 ,  B01F13/0093 ,  B01F2005/0031 ,  B01F2215/0431 ,  B01F2215/0459 ,  B01J2219/00912 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0647 ,  G01N27/44704 ,  G01N27/44726 ,  Y10T436/117497 ,  Y10T436/118339

摘要： This invention provides methods for using liquid junction potentials to control the transport of charged particles in fluid streams that are in laminar flow within microfluidic channels. Applications of the methods of this invention include sample preconditioning (removal of interfering substances), electrophoretic separation (fractionation) of charged particles, enhanced or delayed mixing of charged particles across a fluid interface relative to diffusion only, focusing charged particles in a fluid stream in one or two dimensions, and concentration of charged reactants at a fluid interface.

公开(公告)号：US20120138469A1

公开(公告)日：2012-06-07

申请号：US11939292

申请日：2007-11-13

申请人： Paul Yager ,  Mark Holl ,  Darrel J. Bell ,  James Brody ,  Catherine R. Cabrera ,  Andrew E. Kamholz ,  Katerina Macounova ,  Dong Qin

发明人： Paul Yager ,  Mark Holl ,  Darrel J. Bell ,  James Brody ,  Catherine R. Cabrera ,  Andrew E. Kamholz ,  Katerina Macounova ,  Dong Qin

IPC分类号： B01D57/02

CPC分类号： G01N27/44795 ,  G01N27/44769

摘要： Devices and methods are provided for separation of particles of a first selected electrophoretic mobility or isoelectric point from a fluid comprising particles of at least one other selected electrophoretic mobility or isoelectric point. The devices comprise a microchannel; electrodes to either side of the microchannel for applying a selected voltage to produce an electrical field across the microchannel orthogonal to the length of the microchannel; and outlets in said microchannel placed to receive outlet portions of the fluid containing enhanced concentrations of each type of particle. The devices may be used for particle detection, quantification, separation, mixing, dilution and concentration; to release, separate and detect interior particles of cells or organisms, and to separate particles such as proteins and microorganisms from biological fluids such as blood; or to separate and detect airborne contaminants such as bacterial warfare agents from air.

摘要翻译： 提供了设备和方法，用于从包含至少一个其它选择的电泳迁移率或等电点的颗粒的流体分离第一选择的电泳迁移率或等电点的颗粒。 所述装置包括微通道; 电极到微通道的任一侧，用于施加所选择的电压，以跨过与微通道长度正交的微通道产生电场; 并且所述微通道中的出口被设置成接收含有增强浓度的每种类型的颗粒的流体的出口部分。 该装置可用于颗粒检测，定量，分离，混合，稀释和浓缩; 释放，分离和检测细胞或生物体的内部颗粒，并将诸如蛋白质和微生物的颗粒与生物液体如血液分离; 或从空气中分离和检测空气污染物，如细菌战剂。