公开(公告)号：US06602473B1

公开(公告)日：2003-08-05

申请号：US09545440

申请日：2000-04-07

Applicant: M. Allen Northrup

Inventor： M. Allen Northrup

IPC: B01L300

CPC classification number: B01L7/52 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00824 ,  B01J2219/00828 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00936 ,  B01J2219/00945 ,  B01J2219/00952 ,  B01L3/5027 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0439 ,  B01L2400/0487 ,  B81C3/00 ,  G01N21/0332 ,  G01N21/05 ,  G01N21/69 ,  G01N21/76 ,  G01N2021/0346

Abstract: A silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and non-silicon based materials to provide the thermal properties desired. For example, the chamber may combine a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.

Abstract translation: 一种硅基套管型化学反应室，其结合加热器，例如用于加热的掺杂多晶硅和用于对流冷却的体硅。 反应室结合硅和非硅基材料的临界比以提供所需的热性质。 例如，室可将硅和氮化硅的临界比与待加热材料（例如液体）的体积组合，以便提供均匀的加热，但低功率要求。 反应室还将允许将二次管（例如塑料）引入到包含反应混合物的反应套筒中，从而减轻任何潜在的材料不相容性问题。 反应室可用于任何用于合成或加工有机，无机或生物化学反应的化学反应体系，例如聚合酶链式反应（PCR）和/或其它DNA反应，例如连接酶链式反应，其为实施例 的合成，热循环反应。 反应室也可用于合成仪器，特别是用于DNA扩增和合成的仪器。

公开(公告)号：US20020172969A1

公开(公告)日：2002-11-21

申请号：US10113238

申请日：2002-04-01

Applicant: The Regents of the University of Michigan

Inventor： Mark A. Burns ,  David T. Burke ,  Brian N. Johnson ,  John D. DeNuzzio ,  Wayne F. Beyer JR.

IPC: C12Q001/68 ,  C12M001/34 ,  C12M001/00

CPC classification number: B82Y30/00 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/00986 ,  B01J2219/00995 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2300/165 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0448 ,  B01L2400/0677 ,  B01L2400/0688 ,  C12Q1/6844

Abstract: Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated substrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.

公开(公告)号：US06475363B1

公开(公告)日：2002-11-05

申请号：US09477585

申请日：2000-01-04

Applicant: J. Michael Ramsey

Inventor： J. Michael Ramsey

IPC: G01N27447

CPC classification number: B01L3/5027 ,  B01F13/0059 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/02 ,  G01N30/16 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  Y10S366/01 ,  B01D15/3842

Abstract: A microchip laboratory system and method provide fluid manipulations for a variety of applications, including sample injection for microchip chemical separations. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis and electrochromatography are performed in channels formed in the substrate. Analytes are loaded into a four-way intersection of channels by electrokinetically pumping the analyte through the intersection, followed by switching of the potentials to force an analyte plug into the separation channel.

Abstract translation: 微芯片实验室系统和方法为各种应用提供流体操作，包括用于微芯片化学分离的样品注入。 使用标准光刻工艺和化学湿法蚀刻制造微芯片，其中基板和盖板通过直接接合连接。 毛细管电泳和电色谱在基底中形成的通道中进行。 通过电化学地将分析物泵送通过交叉点，将分析物加载到四通道通道中，然后切换电位以迫使分析物插塞进入分离通道。

公开(公告)号：US20020008030A1

公开(公告)日：2002-01-24

申请号：US09909638

申请日：2001-07-20

Inventor： J. Michael Ramsey

IPC: G01N027/26 ,  G01N027/447

CPC classification number: B01L3/5027 ,  B01F13/0059 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/02 ,  G01N30/16 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  Y10S366/01 ,  B01D15/3842

Abstract: A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.

Abstract translation: 微芯片装置和方法为各种应用提供流体操作，包括用于微芯片液相色谱的样品注入。 使用标准光刻工艺和化学湿法蚀刻制造微芯片，其中基板和盖板通过直接接合连接。 在基底中形成的通道中进行毛细管电泳。 注射通过电渗透泵送样品通过穿过分离通道的注入通道，然后切换电位以迫使插塞进入分离通道。

公开(公告)号：US5882496A

公开(公告)日：1999-03-16

申请号：US807152

申请日：1997-02-27

Applicant: M. Allen Northrup ,  Conrad M. Yu ,  Norman F. Raley

Inventor： M. Allen Northrup ,  Conrad M. Yu ,  Norman F. Raley

IPC: B01D39/20 ,  B01D57/02 ,  B01D71/02 ,  B01J19/00 ,  B01L3/00 ,  G01N27/447 ,  G01N27/27 ,  B01D24/00 ,  B01D53/22

CPC classification number: B01D67/0044 ,  B01D39/2006 ,  B01D57/02 ,  B01D71/02 ,  B01J19/0093 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  G01N27/44743 ,  G01N27/44791 ,  B01D2325/04 ,  B01J2219/00828 ,  B01J2219/00873 ,  B01J2219/00905 ,  B01J2219/00912 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0442 ,  B01L2400/0688 ,  Y10S977/832 ,  Y10S977/888

Abstract: Fabrication and use of porous silicon structures to increase surface area of heated reaction chambers, electrophoresis devices, and thermopneumatic sensor-actuators, chemical preconcentrates, and filtering or control flow devices. In particular, such high surface area or specific pore size porous silicon structures will be useful in significantly augmenting the adsorption, vaporization, desorption, condensation and flow of liquids and gasses in applications that use such processes on a miniature scale. Examples that will benefit from a high surface area, porous silicon structure include sample preconcentrators that are designed to adsorb and subsequently desorb specific chemical species from a sample background; chemical reaction chambers with enhanced surface reaction rates; and sensor-actuator chamber devices with increased pressure for thermopneumatic actuation of integrated membranes. Examples that benefit from specific pore sized porous silicon are chemical/biological filters and thermally-activated flow devices with active or adjacent surfaces such as electrodes or heaters.

Abstract translation: 制造和使用多孔硅结构以增加加热的反应室，电泳装置和热气传感器致动器，化学预浓缩物和过滤或控制流动装置的表面积。 特别地，这种高表面积或特定孔径的多孔硅结构在用于微小尺度的这些方法的应用中将可用于显着增加液体和气体的吸附，汽化，解吸，冷凝和流动。 将受益于高表面积的多孔硅结构的实例包括样品预浓缩器，其设计成吸附并随后从样品背景中解吸特定化学物质; 具有增强的表面反应速率的化学反应室; 以及具有增加压力的传感器致动器室装置，用于集成膜的热气动致动。 受益于特定孔径多孔硅的实例是化学/生物过滤器和具有活性或相邻表面的热活化流动装置，例如电极或加热器。

公开(公告)号：US08039205B2

公开(公告)日：2011-10-18

申请号：US11948891

申请日：2007-11-30

Applicant: Chien-Hua Chen ,  Xia feng Yang

Inventor： Chien-Hua Chen ,  Xia feng Yang

IPC: G03F7/20

CPC classification number: F04B43/046 ,  B01D57/02 ,  B01D61/18 ,  B01D63/081 ,  B01D63/088 ,  B01D67/003 ,  B01D2323/30 ,  B01D2323/50 ,  B01D2325/08 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/0081 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01J2219/00912 ,  B01J2219/00943 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0439 ,  B01L2400/0442 ,  B01L2400/0638 ,  B81B2201/058 ,  B81C1/00119 ,  F04B19/24 ,  G01N21/05 ,  G01N2021/0346 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: A method includes depositing a sacrificial material on a substrate, and depositing a polymer layer on the substrate and the sacrificial material. The method further includes removing the sacrificial material to at least partially define boundaries of at least one fluidic channel of a fluidic micro electromechanical system (MEM) device, the at least one fluidic channel is at least partially defined by a portion of the polymer layer and a portion of the substrate.

Abstract translation: 一种方法包括将牺牲材料沉积在基底上，以及在基底和牺牲材料上沉积聚合物层。 该方法还包括去除牺牲材料以至少部分地限定流体微机电系统（MEM）装置的至少一个流体通道的边界，所述至少一个流体通道至少部分地由聚合物层的一部分限定， 衬底的一部分。

公开(公告)号：US07309467B2

公开(公告)日：2007-12-18

申请号：US10606023

申请日：2003-06-24

Applicant: Chien-Hua Chen ,  Xia feng Yang

Inventor： Chien-Hua Chen ,  Xia feng Yang

IPC: B01L3/02 ,  B01L11/00 ,  B32B5/02 ,  B32B27/04 ,  B32B27/12

CPC classification number: F04B43/046 ,  B01D57/02 ,  B01D61/18 ,  B01D63/081 ,  B01D63/088 ,  B01D67/003 ,  B01D2323/30 ,  B01D2323/50 ,  B01D2325/08 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/0081 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01J2219/00912 ,  B01J2219/00943 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0439 ,  B01L2400/0442 ,  B01L2400/0638 ,  B81B2201/058 ,  B81C1/00119 ,  F04B19/24 ,  G01N21/05 ,  G01N2021/0346 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: A fluidic micro electro-mechanical system (MEMS) device is described. In one aspect, at least one at least partially covered fluidic channel is formed between a polymer layer and a polymer substrate as the polymer layer is deposited on the substrate. The partially covered fluidic channel is fabricated as a unitary polymer layer structure. In one implementation, a strong exposure process is applied to the polymer layer to create a deep cross-linked polymer region. A weak exposure process is applied to the polymer layer to create a shallow cross-linked polymer region.

Abstract translation: 描述了流体微机电系统（MEMS）装置。 在一个方面，当聚合物层沉积在基底上时，在聚合物层和聚合物基底之间形成至少一个至少部分覆盖的流体通道。 部分覆盖的流体通道被制造为单一的聚合物层结构。 在一个实施方案中，将强烈的曝光工艺应用于聚合物层以产生深交联聚合物区域。 将弱曝光工艺应用于聚合物层以产生浅交联聚合物区域。

公开(公告)号：US07156969B2

公开(公告)日：2007-01-02

申请号：US10701652

申请日：2003-11-05

Applicant: Tammy Burd Mehta ,  Anne R. Kopf-Sill

Inventor： Tammy Burd Mehta ,  Anne R. Kopf-Sill

IPC: G01N27/26

CPC classification number: G01N27/44791 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01J2219/00912 ,  B01J2219/00961 ,  B01J2219/00986 ,  B01L3/5027 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  Y10S435/814

Abstract: Multiphasic microfluidic apparatus for performing product fluid manipulation and separation in a single continuous unit are provided. Related methods, kits, and compositions are also provided.

Abstract translation: 提供了用于在单个连续单元中执行产品流体操纵和分离的多相微流体装置。 还提供了相关方法，试剂盒和组合物。

公开(公告)号：US06733730B1

公开(公告)日：2004-05-11

申请号：US09707337

申请日：2000-11-06

Applicant: Stewart K. Griffiths ,  Robert H. Nilson

Inventor： Stewart K. Griffiths ,  Robert H. Nilson

IPC: B01L302

CPC classification number: G01N30/6086 ,  B01F5/0403 ,  B01F5/0646 ,  B01F5/0647 ,  B01F5/0652 ,  B01F13/0059 ,  B01F2215/0037 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00957 ,  B01L3/502746 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N27/44791 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/285 ,  Y10S366/02 ,  Y10S366/03

Abstract: What is disclosed pertains to improvement in the performance of microchannel devices by providing turns, wyes, tees, and other junctions that produce little dispersion of a sample as it traverses the turn or junction. The reduced dispersion results from contraction and expansion regions that reduce the cross-sectional area over some portion of the turn or junction. By carefully designing the geometries of these regions, sample dispersion in turns and junctions is reduced to levels comparable to the effects of ordinary diffusion. The low dispersion features are particularly suited for microfluidic devices and systems using either electromotive force, pressure, or combinations thereof as the principle of fluid transport. Such microfluidic devices and systems are useful for separation of components, sample transport, reaction, mixing, dilution or synthesis, or combinations thereof.

Abstract translation: 所公开的内容涉及通过提供匝，纱，三通和其他在样品穿过匝或接合点时几乎不产生分散的结的性能来提高微通道器件的性能。 缩小的分散体是由收缩和膨胀区域引起的，这些区域减小了转弯或结合部分的横截面面积。 通过仔细设计这些区域的几何形状，轮流和接头的样品分散度降低到与普通扩散效果相当的水平。 低分散特征特别适用于使用电动势，压力或其组合作为流体输送原理的微流体装置和系统。 这种微流体装置和系统可用于分离组分，样品转运，反应，混合，稀释或合成或其组合。

公开(公告)号：US20040007464A1

公开(公告)日：2004-01-15

申请号：US10434874

申请日：2003-05-09

Inventor： J. Michael Ramsey

IPC: G01N027/27

CPC classification number: B01L3/5027 ,  B01F13/0059 ,  B01F13/0062 ,  B01F13/0076 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00916 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01J2219/00995 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B29C65/4895 ,  B29C66/026 ,  B29C66/034 ,  B29C66/54 ,  G01N27/44743 ,  G01N27/44791 ,  G01N30/02 ,  G01N30/16 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/162 ,  G01N2030/285 ,  G01N2030/383 ,  G01N2030/8435 ,  Y10S366/01 ,  B01D15/3842

Abstract: A microchip apparatus and method provide fluidic manipulations for a variety of applications, including sample injection for microchip liquid chromatography. The microchip is fabricated using standard photolithographic procedures and chemical wet etching, with the substrate and cover plate joined using direct bonding. Capillary electrophoresis is performed in channels formed in the substrate. Injections are made by electro-osmotically pumping sample through the injection channel that crosses the separation channel, followed by a switching of the potentials to force a plug into the separation channel.

Abstract translation: 微芯片装置和方法为各种应用提供流体操作，包括用于微芯片液相色谱的样品注入。 使用标准光刻工艺和化学湿法蚀刻制造微芯片，其中基板和盖板通过直接接合连接。 在基底中形成的通道中进行毛细管电泳。 注射通过电渗透泵送样品通过穿过分离通道的注入通道，然后切换电位以迫使插塞进入分离通道。